Growing New Kidneys from Embryonic Cell Suspensions

D’Agati, Vivette D.

doi:10.1681/asn.2012090888

Cited by 10 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the major roadblocks to the development of successful therapeutics for CKD depends on the ability to effectively establish 3D models that can mimic the complex structure and function of the GFB. Even though some success in generating kidney structures has been described using conventional 2D or 3D culture systems (including spheroids and extracellular based gels 5 ), the results are still inconsistent. The recent discovery of kidney organoids allows the formation of nephron-like structures that recapitulate some of the characteristics of the glomerular environment 6 , but they have no or limited filtration activity and the deposition of a correct GBM has not been fully demonstrated yet.…”

Section: Introductionmentioning

confidence: 99%

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier

et al. 2019

View full text Add to dashboard Cite

In this work we model the glomerular filtration barrier, the structure responsible for filtering the blood and preventing the loss of proteins, using human podocytes and glomerular endothelial cells seeded into microfluidic chips. In long-term cultures, cells maintain their morphology, form capillary-like structures and express slit diaphragm proteins. This system recapitulates functions and structure of the glomerulus, including permselectivity. When exposed to sera from patients with anti-podocyte autoantibodies, the chips show albuminuria proportional to patients’ proteinuria, phenomenon not observed with sera from healthy controls or individuals with primary podocyte defects. We also show its applicability for renal disease modeling and drug testing. A total of 2000 independent chips were analyzed, supporting high reproducibility and validation of the system for high-throughput screening of therapeutic compounds. The study of the patho-physiology of the glomerulus and identification of therapeutic targets are also feasible using this chip.

show abstract

Section: Introductionmentioning

confidence: 99%

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Unilateral nephrectomy was performed to enhance the potential for growth and development of the implanted tissue. To promote endogenous vascular development, the authors developed a new technique of preconditioning the organoids in culture with vascular endothelial growth factor (VEGF) followed by injection of VEGF locally into the area of implantation as well as intravenous injections into the tail vein three times each week [28, 29]. Unfortunately, the organoids were only viable for 3–4 weeks before they began to involute.…”

Section: Whole-kidney Tissue Engineeringmentioning

confidence: 99%

Regenerating a kidney in a lymph node

Francipane

Lagasse

2015

Pediatr Nephrol

View full text Add to dashboard Cite

The ultimate treatment for end-stage renal disease (ESRD) is orthotopic transplantation. However, the demand for kidney transplantation far exceeds the number of available donor organs. While more than 100,000 Americans need a kidney, only 17,000 people receive a kidney transplant each year (National Kidney Foundation’s estimations). In recent years, several regenerative medicine/tissue engineering approaches have been exploited to alleviate the kidney shortage crisis. Although these approaches have yielded promising results in experimental animal models, the kidney is a complex organ and translation into the clinical realm has been challenging to date. In this review, we will discuss cell therapy-based approaches for kidney regeneration and whole-kidney tissue engineering strategies, including our innovative approach to regenerate a functional kidney using the lymph node as an in vivo bioreactor.

show abstract

“…Approaches starting from the metanephric kidney and dissociating down to the single-cell level provide a valuable window for performing pharmacologic or genetic modifications in individual cells prior to the in vitro aggregation step [13,24]. For instance, the single-cell stage allows the use of genetic engineering approaches to ‘humanize' cells, possibly by introducing immunomodulatory genes or by switching off others, to reduce the possibility of rejection and facilitate the xenotranplantation of animal renal tissues [24].…”

Section: Generation Of Kidney Tissue From Metanephric Progenitor Cellsmentioning

confidence: 99%

“…For instance, the single-cell stage allows the use of genetic engineering approaches to ‘humanize' cells, possibly by introducing immunomodulatory genes or by switching off others, to reduce the possibility of rejection and facilitate the xenotranplantation of animal renal tissues [24]. Self-forming kidneys can be also exploited to generate human renal tissue by first constructing chimeric kidneys that combine animal progenitor cells and human stem cells, followed by the selective elimination of animal cells after transplantation.…”

Section: Generation Of Kidney Tissue From Metanephric Progenitor Cellsmentioning

confidence: 99%

Reforming the Kidney Starting from a Single-Cell Suspension

Xinaris

Yokoo

2014

Nephron Exp Nephrol

View full text Add to dashboard Cite

Background: Chronic kidney disease affects 5-7% of people worldwide. The increasing number of patients and the shortage of transplantable organs create an imperative need to develop new methods for generating kidney tissue. Summary: Recent advances in our understanding of the developmental biology of the kidney, along with the establishment of novel methodologies in the field of regenerative medicine, have created significant potential for kidney regeneration. These advances incorporate both transplantation of metanephric primordia into adult recipients and construction of ‘fetal' kidney tissue from suspensions of single cells of metanephric origin. This paper examines these approaches in the context of organ regeneration. Key Messages: The use of transplants of metanephric origin has the advantage over undifferentiated stem cells of already being committed to a renal developmental program. Although several technical difficulties remain to be overcome, the validation of these systems in preclinical models of renal disease will be of decisive importance in the coming years.

show abstract

Growing New Kidneys from Embryonic Cell Suspensions

Cited by 10 publications

References 25 publications

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier

Regenerating a kidney in a lymph node

Reforming the Kidney Starting from a Single-Cell Suspension

Contact Info

Product

Resources

About