Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte

Luhan, Maria Rovilla J.; Sollesta, Hananiah

doi:10.1007/s10811-009-9497-7

Cited by 40 publications

(4 citation statements)

References 19 publications

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“…An even higher growth rate of 34%/d in K. striatum (sporophytes) was recorded when first transferred from aquarium to tank (Luhan and Sollesta 2010), but this rate decreased in the succeeding measurements before the plantlets were transferred to the net cage under natural conditions. Growth in the laboratory and land-based facility was impressive both in the study of Hinaloc and Roleda (2021) and Luhan and Sollesta (2010). However, when transplanted into the natural condition, the growth rate decreased to 2.7%/d in K. striatum (Luhan and Sollesta 2010), becoming similar to that (2.6%/d) obtained in the present study for sporophytes of K. alvarezii grown under the same condition.…”

Section: Resultssupporting

confidence: 84%

Growth and Carrageenan Quality of Sporophyte and Gametophyte of the Commercially Important Red Seaweed Kappaphycus alvarezii

Luhan¹,

Mateo²,

Sollesta-Pitogo³

2022

Philipp J Sci

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One dilemma facing the farming of the carrageenophyte Kappaphycus is the deteriorating quality of seedstock. Rejuvenating the commercial stocks using spores from reproductive wild parents that are sexually and asexually produced could perhaps restore the traits that are useful for culture. The use of gametophyte and sporophyte (=tetrasporophyte) phases of Kappaphycus alvarezii from spores is explored as a source of seedstock. The growth and physical properties of semi-refined carrageenan extracted from the resulting sporophyte and gametophyte thalli of the same ages were determined. The growth rate of the diploid tetrasporophyte thalli was significantly higher than that of the haploid gametophyte. Gel strength and yield of semi-refined carrageenan from the two life cycle phases were not significantly different, whereas the viscosity was significantly higher in the diploid tissues. Kappaphycus sporophytes were potentially superior to gametophytes as a source of seedstock for mariculture, but problems need to be addressed – including improving the growth rate and reducing the cost of production – before its application could be realized.

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Section: Resultssupporting

confidence: 84%

Growth and Carrageenan Quality of Sporophyte and Gametophyte of the Commercially Important Red Seaweed Kappaphycus alvarezii

Luhan¹,

Mateo²,

Sollesta-Pitogo³

2022

Philipp J Sci

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“…Macroalgae were dried with absorbent paper and wet weights (g/L) were determined on a digital balance, with four consecutive weights of the algal group recorded for each replicate. The average (n = 4) biomass values were used to calculate the specific growth rate (SGR) (Luhan and Sollesta, 2010;Yong et al, 2013), which was estimated using the formula SGR = (ln Mtln Mo)/T x 100%, where R = relative growth rate, Mo = initial dry mass, Mt = dry mass measured on day t, and T = number of days.…”

Section: Biomass and Phmentioning

confidence: 99%

Influence of laboratory conditions on two species Dictyotaceae family (Phaeophyceae): Diterpenoid profile and physiological response

et al. 2023

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The seaweed Dictyotaceae family has a great wealth of diterpenes with important biological activities. Information on these diterpenes qualitative and quantitative aspects in conjunction with physiological data and abiotic parameters are essential to obtain an effective algal culture system that is a sustainable resource. In this study, the diterpene profile of Dictyota menstrualis and Canistrocarpus cervicornis were analyzed as a function of in vitro maintenance time and the influence of culture medium. The algae were collected in the state of Rio de Janeiro and subjected to an acclimation period in the laboratory. Then, the species were acclimated in aquaria containing seawater from the collection sites. Then biomass algal was divided into (i) control experiment (CC): Growth in sterilized seawater; (ii) enriched experiment (EE): Growth in sterilized seawater enriched with Provasoli/2. Triplicate experiments were maintained in vitro (2.4% m/v), and the medium was changed every 7 days. Diterpenes were monitored using dichloromethane extracts from dried algae: (a) collected in the field, (b) after the acclimation period, and (c) from experimental units taken every 7 days for a total of 21 days. Among the main results, we found response variables such as specific growth rate (SGR); moreover, positive data were obtained for the alga C. cervicornis in CC, and this was evident showing after 7 days of cultivation in the experimental control unit (CU). For D. menstrualis, the SGRs for the treatments were negative at the monitored time, which was due to biomass losses, frond breakage, and rapid decomposition of some individuals that did not withstand the stress conditions during their collection. The analysis of the chemical profile by GC-MS made it possible to monitor 12 diterpenes and one sterol in the alga D. menstrualis and 7 diterpenes in C. cervicorni. The correlations found by Generalized Linear Models (GLMs) and redundancy analysis (RDA) statistical analyzes between the diterpene profile with the monitored variables (Nitrate, ammonia and pH), showed correlations for 10 diterpenes of the species D. menstrualis and 1 of the C. cervicornis, our analyses are important for the discussion of metabolic plasticity of the Dictyotaceae family

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“…Since the original seedstocks were sourced from a few genotypes, their continuous vegetative propagation resulted in the low genetic diversity of the cultivars (Zuccarello et al 2006, Halling et al 2013. To sustain the carrageenan industry, it is essential to generate or discover new strains / seedstocks from the wild (e.g., Azanza-Corrales and Ask 2003, Luhan and Sollesta 2010, Roleda et al 2017.…”

Section: Sample Collectionmentioning

confidence: 99%

Discovery of novel haplotypes from wild populations of Kappaphycus (Gigartinales, Rhodophyta) in the Philippines

Roleda¹,

Aguinaldo²,

Crisostomo³

et al. 2021

ALGAE

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As the global demand for the carrageenophyte Kappaphycus is steadily increasing, its overall productivity, carrageenan quality, and disease resistance are gradually declining. In the face of this dilemma, wild Kappaphycus populations are viewed as sources of new cultivars that could potentially enhance production; therefore, assessment of their diversity is crucial. This study highlights the morphological and genetic diversity of wild Kappaphycus species obtained from two sites in the Philippines. Nucleotide alignments of available 5′ region of the mitochondrial cytochrome c oxidase subunit I (COI-5P) and cox2-3 spacer sequences of Kappaphycus confirmed the presence of K. alvarezii in Guiuan, Eastern Samar and K. striatus in Bolinao, Pangasinan. Based on the concatenated sequences of the COI-5P and the cox2-3 spacer, nine novel haplotypes were observed along with other published haplotypes. However, there was no relationship between haplotype and morphology. These newly recognized haplotypes indicate a reservoir of unutilized wild genotypes in the Philippines, which could be taken advantage of in developing new cultivars with superior traits. DNA barcodes generated from this study effectively expand the existing databank of Kappaphycus sequences and can provide insights in elucidating the genetic diversity of Kappaphycus species in the country.

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Growing the reproductive cells (carpospores) of the seaweed, Kappaphycus striatum, in the laboratory until outplanting in the field and maturation to tetrasporophyte

Cited by 40 publications

References 19 publications

Growth and Carrageenan Quality of Sporophyte and Gametophyte of the Commercially Important Red Seaweed Kappaphycus alvarezii

Growth and Carrageenan Quality of Sporophyte and Gametophyte of the Commercially Important Red Seaweed Kappaphycus alvarezii

Influence of laboratory conditions on two species Dictyotaceae family (Phaeophyceae): Diterpenoid profile and physiological response

Discovery of novel haplotypes from wild populations of Kappaphycus (Gigartinales, Rhodophyta) in the Philippines

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