Growth&amp;ndash;Inhibitory Effect of Prostasomes on Prostatic Cancer Cell Lines in Culture

Carlsson, Lena; Lennartsson, Lena; Nilsson, Berith; Nilsson, Sten; Ronquist, Gunnar

doi:10.1159/000020326

Cited by 16 publications

(11 citation statements)

References 43 publications

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“…Despite this, relatively little work has been published on the area. Carlsson et al 13 have shown that prostasomes exert a growth-inhibitory effect on a range of prostate cancer cell lines in culture: our own work has confirmed this.…”

Section: Introductionsupporting

confidence: 76%

In vitro inhibition of angiogenesis by prostasomes

Delves

Stewart²,

Lwaleed

et al. 2005

Prostate Cancer Prostatic Dis

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Prostasomes are biologically active organelles that are secreted by human prostate epithelial cells, and it is believed that they have a role in prostatic disease. We studied the effect of prostasomes on the human umbilical vein endothelial cell (HUVEC)/Matrigel model of angiogenesis, and the association of labelled prostasomes with HUVECs. The growth inhibitory effect of prostasomes on HUVECs was assayed by spectrophotometric measurement of residual biomass. Preparations of HUVECs on a Matrigel base were exposed to prostasomes, and the development of capillary-like networks was quantified. Prostasomes were labelled with PKH-26, and cultured with HUVECs. Prostasomes were not shown to have a significant effect on HUVEC survival. Angiogenesis assays showed inhibition. The PKH-26-labelled particles were shown to have adhered to the HUVECs. This study adds the inhibition of an in vitro correlate of angiogenesis to the known actions of prostasomes.

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Section: Introductionsupporting

confidence: 76%

In vitro inhibition of angiogenesis by prostasomes

Delves

Stewart²,

Lwaleed

et al. 2005

Prostate Cancer Prostatic Dis

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“…11 Despite this there has been relatively little experimental evidence to either confirm or refute these views, beyond a study demonstrating that semenderived prostasomes exert a growth-inhibitory effect on prostate cancer cells grown in culture. 12 We have previously examined the influence of prostasomes on angiogenesis, demonstrating interaction of prostasomes with endothelial cells and the inhibition of tubule formation by human umbilical vein endothelial cells (HUVEC) grown on matrigel. 13 In this study, we have now studied the effect of prostasomes on angiogenesis in the rat aortic ring assay, which is a closer reflection of the in vivo situation, and tried to examine the mechanism(s) underlying any effect.…”

Section: Introductionmentioning

confidence: 99%

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Delves

Goyal

Lwaleed³

et al. 2006

Prostate Cancer Prostatic Dis

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Prostasomes are organelles secreted by prostatic epithelial cells, and are believed to have a role in fertility and prostatic disease. They are known to influence sperm motility and the acrosome reaction, and are thought to have a role in cell transformation, immunosuppression, proliferation, facilitation of local invasion, and angiogenesis. Previously, we have demonstrated the inhibitory effect of prostasomes derived from human semen on angiogenesis using HUVEC cells grown on matrigel. In this study, we use the rat aortic ring assay system, arguably a closer reflection of the in vivo situation. Quantification was by a spectrophotometric method, and underlying mechanisms assessed. Prostasomes demonstrated a clear inhibition of angiogenesis, and this effect persisted after heat treatment of prostasomes to denature protein. This fits with other known effects of prostasomes known to be due to the membrane lipid component, which is unusually high in sphingomyelin and cholesterol.

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“…So the apoptosis-inducing effect of CMTM5-v1 may be cell-type specific, which needs further study. It has been reported that prostasomes belong to membranous vesicles, which are produced by epithelial cells of prostate gland and has inhibiting effects on the growth of prostate carcinoma cells especially Du145 cells (21,22). Therefore, it can be inferred that CMTM5-v1-containing vesicles might have more obvious effects, such as inhibiting the proliferation and migration of PC-3 cells and initiating apoptosis in Du145 cells.…”

Section: Discussionmentioning

confidence: 99%

CMTM5-v1, a four-transmembrane protein, presents a secreted form released via a vesicle-mediated secretory pathway

Li¹,

Guo²,

Shao³

et al. 2010

BMB Reports

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The CKLF-like MARVEL transmembrane domain-containing family (CMTM) is a novel family of proteins linking classical chemokines and the transmembrane 4 superfamily (TM4SF). Our earlier studies indicated several CMTM members (such as CKLF1 and CMTM2) have a secreted form. This is the first report of the secreted form of CMTM5-v1, the major RNA splicing form of CMTM5, which is produced as small vesicles (＜100 nm diameter) and floats at a peak density of 1.19 g/ml on continuous sucrose gradients. CMTM5-v1 has no obvious co-localization with CD63 or Golgi complex. In addition, brefeldin A but not wortmannin can inhibit the secretion of CMTM5-v1. Our results suggest that CMTM5-v1 might be secreted via a different vesicle-mediated secretory pathway, which will be helpful for the studies of vesicle-mediated secretion and MARVEL domain-containing proteins. [BMB reports 2010; 43(3): 182-187]

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Growth–Inhibitory Effect of Prostasomes on Prostatic Cancer Cell Lines in Culture

Cited by 16 publications

References 43 publications

In vitro inhibition of angiogenesis by prostasomes

In vitro inhibition of angiogenesis by prostasomes

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

CMTM5-v1, a four-transmembrane protein, presents a secreted form released via a vesicle-mediated secretory pathway

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