Growth and Biochemical Variations in Macrotyloma Uniflorum Var. Madhu Under Chromium Stress

Abstract

The discharge of contaminated mining and industrial residues has become a major cause of environmental pollution. Seed is highly protective against external metal stresses. But they are highly sensitive during vegetative developmental process. In this study, germination was conducted in Macrotyloma uniflorum var. Madhu, in order to find out the effect of chromium toxicity on germination, growth and biochemical variations. The seeds were germinated in five different concentrations of chromium (25ìM, 50ìM, 100ìM, 150ìM and 200 ìM) solution. The hydroponically grown plants revealed the toxic phenotypic expression (root length, shoot length, seedling length, shoot vigor index, shoot fresh weight, shoot dry weight, root fresh weight and root dry weight) of plant after 3 growth stage (15 d, 30 d and 45 d). Similarly biochemical characteristics as well as antioxidant activities (Chlorophyll, carotenoid, carbohydrate, reducing sugar, catalase, proline content and peroxidase) were studied to see the toxic effect of chromium.

Alleviating Cr(VI) stress in horse gram (Macrotyloma uniflorum Var. Madhu) by native Cr-tolerant nodule endophytes isolated from contaminated site of Sukinda

“…Furthermore, higher concentrations of soil available Cr was recorded in pots treated with C 1 (15 mg kg −1 Cr 6+ ) which were found to be at par with the control pots (C 0 ). Previous research observations from Patra et al ( 2018 ) and Dhali et al ( 2020 ) corroborate with our findings.…”

“…6 ). Cr toxicity adversely affects total chlorophyll concentration, proteins, sugar and carbohydrates (Rai et al 2014 ; Dhali et al 2020 ; Sharma et al 2020 ). Plants growing under Cr stress were assayed with declining biosynthesis of chlorophyll pigments (Sharma et al 2019 ).…”

“…Hexavalent chromium (Cr(VI)) toxicity in soil induces severe damages to the plant. Cr(VI) is reduced to other oxidation states after entering the plant cell and attacks cellular proteins, DNA, and membrane proteins ultimately leading to physiological and morphological stress in various crops (Stambulska et al 2018 ; Panda et al 2020 ; Dhali et al 2020 ).…”

Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield

“…Each bag and bottle were inoculated with liquid spawn and incubated in the dark at 25 • C. For bottle cultivation, the mycelial growth rates of wild-type strains and transformants were measured and recorded. Moreover, the extracellular metabolic parameters, including soluble protein, reducing sugar, and the activity of CMCase, FPase, xylanase, and amylase, by four transformants and wild-type strains, were determined as described in previous studies when mycelial filled half of the cultivation medium in a bottle (Dhali et al, 2021). For bag cultivation, the bags were transferred to the fruiting room for fruiting after overgrowing and ripening (110 days).…”