Growth and Cultural Characteristics ofOphiocordyceps longissimaCollected in Korea

Abstract

We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of 25℃ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, K3PO4 showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and K3PO4 (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.

Mycelial Growth of <i>Paecilomyces hepiali</i> in Various Agar Media and Yield of Fruit Bodies in Rice Based Media

“…The highest colony growth was obtained at pH 7. This was similar to previous studies on other fungal species [16][17][18]. However, pH 7 was not statistically different from pH 6 and 8 (p > 0.05).…”

“…This is same as findings reported on other fungal species namely Cordyceps nutans Pat. [16], Ophiocordyceps longissima [17], Ophiocordyceps heteropoda [18], Cordyceps cardinalis [19] and Cordyceps sinensis (now known as Ophiocordyceps sinensis) [20]. It was interest- A. CHIOZA, S. OHGA Open Access AiM 532 ing to note that even at 5˚C there was some growth at the rate of around 0.89 mm/day in terms of colony diameter.…”

“…This is similar to findings in studies of other fungi. Sung et al [17] reported that nitrogen sources such as yeast extract, peptone and tryptone are favourable for mycelial growth of O. longissima as in other Cordyceps and allied species. Dong and Yao [21] pointed out that C. sinensis had greater preference on organic nitrogen, which is common in fungi.…”

Coleopteran and Lepidopteran Hosts of the Entomopathogenic Genus Cordyceps sensu lato

“…Cordyceps fungi range in habitat from aerial to epigeal to subterranean ones [74], based on their respective host species. When the host stage is adult, they grow on exposed environments such as leaf litter or are attached to some plant parts such as leaf and branch and in contrast to the larval or pupal stages that are buried in soil (hypogeal) or on wood up to nearly 50 cm, for example, O. longissima [75] and O.…”

Molecular identification and optimization of cultural conditions for mycelial biomass production of wild strain of Chlorophyllum molybdites (G.Mey) Massee from the Philippines

“…In addition, findings of Liu et al [43] revealed that illumination does not affect the mycelial growth of Isaria farinosa on PDD. Furthermore, Sung et al [44] observed no obvious difference in the colony diameter of Ophiocordyceps longissima between light and dark conditions on solid media. In contrary, Kalaw et al [30] revealed that illumination significantly influenced the mycelial growth of five mushroom species on solid medium: L. sajor-caju and S. commune (CLSU strain), whereas dark condition showed maximum growth of G. lucidum (strain B), L. tigrinus (CLSU strain), G. lucidum (strain A), and C. cinerea (Sto.…”