Growth and eruption of gel-entrapped microcolonies

Hüsken, Leonie E.; Tramper, J.; Wijffels, René H.

doi:10.1016/s0921-0423(96)80045-1

Cited by 9 publications

(6 citation statements)

References 3 publications

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“…(iii) Detached cells: expanding colonies near the surface of the gel will eventually touch the gel surface and pieces of biomass could be released. In beads, it seems that dispersed cells are an eruption of entire microcolonies at once into the surrounding medium [ 66 , 69 – 71 ] (Fig. 3 ).…”

Section: Biofilms As Cell Factoriesmentioning

confidence: 99%

Living together in biofilms: the microbial cell factory and its biotechnological implications

2016

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In nature, bacteria alternate between two modes of growth: a unicellular life phase, in which the cells are free-swimming (planktonic), and a multicellular life phase, in which the cells are sessile and live in a biofilm, that can be defined as surface-associated microbial heterogeneous structures comprising different populations of microorganisms surrounded by a self-produced matrix that allows their attachment to inert or organic surfaces. While a unicellular life phase allows for bacterial dispersion and the colonization of new environments, biofilms allow sessile cells to live in a coordinated, more permanent manner that favors their proliferation. In this alternating cycle, bacteria accomplish two physiological transitions via differential gene expression: (i) from planktonic cells to sessile cells within a biofilm, and (ii) from sessile to detached, newly planktonic cells. Many of the innate characteristics of biofilm bacteria are of biotechnological interest, such as the synthesis of valuable compounds (e.g., surfactants, ethanol) and the enhancement/processing of certain foods (e.g., table olives). Understanding the ecology of biofilm formation will allow the design of systems that will facilitate making products of interest and improve their yields.

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Section: Biofilms As Cell Factoriesmentioning

confidence: 99%

Living together in biofilms: the microbial cell factory and its biotechnological implications

2016

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“…As the colony expands, it may eventually reach the surface of the gel bead. This leads to an eruption of the colony in which the contents of the colony are released to the surrounding medium (Hüsken et al . 1996).…”

Section: Introductionmentioning

confidence: 99%

pH-controlled cell release and biomass distribution of alginate-immobilized Lactococcus lactis subsp. lactis

et al. 2001

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Aims: To investigate the growth and release of Lactococcus lactis subsp. lactis in gel beads and to affect rates of cell release by changing the growth conditions. Methods and Results: The rate of release and the distribution of immobilized L. lactis subsp. lactis in alginate beads were studied in continuous fermentations for 48 h. A change in operating pH from 6á5 to 9á25 initially reduced the ratio of the rates of cell release to lactate production by almost a factor of 10 5 . Compared with fermentations at pH 6á5, growth at pH 9á25 also increased the ®nal internal bead biomass concentration by a factor of 5 and increased the ®nal rate of lactate production by 25%. After 48 h, the ratio of the rates of cell release to lactate production was still 10 times lower than in fermentations at pH 6á5. Conclusions: A change in the operating pH from 6á5 to 9á25 reduced rates of cell release throughout 48 h of fermentation and increased the ®nal rates of lactate production and internal bead biomass concentration. Signi®cance and Impact of the Study: These data illustrate that diffusional limitations and corresponding pH gradients can be exploited in affecting the distribution of immobilized growing cells and their concomitant release.

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“…Inside soft organic polymers like alginate, cells proliferate and form colonies (Hüsken et al 1996), which have never been observed in a silica gel. In general, silica matrices, due to the zero Young modulus of elasticity, are believed to disable cell proliferation (Inama et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Bioluminescence of Pseudomonas fluorescens HK44 in the course of encapsulation into silica gel. Effect of methanol

2010

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The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)₄. The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-3 and 8-18 %, respectively; both remained lowered as long as the silica biofilm contained residual MeOH. The kinetics of MeOH being released from silica biofilms (a thickness of 2-6 mm) were first-order. The decrease of bacterial activity due to encapsulation was proportional to the biofilm thickness. MeOH evolving during encapsulation is probably the principal stress factor but not the only one.

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Growth and eruption of gel-entrapped microcolonies

Cited by 9 publications

References 3 publications

Living together in biofilms: the microbial cell factory and its biotechnological implications

Living together in biofilms: the microbial cell factory and its biotechnological implications

pH-controlled cell release and biomass distribution of alginate-immobilized Lactococcus lactis subsp. lactis

Bioluminescence of Pseudomonas fluorescens HK44 in the course of encapsulation into silica gel. Effect of methanol

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