Growth and Freeze-Drying Optimization of &amp;lt;i&amp;gt;Bifidobacterium crudilactis&amp;lt;/i&amp;gt;

Tanimomo, J.; Delcenserie, Véronique; Taminiau, Bernard; Daube, Georges; Saint-Hubert, Catherine; Durieux, Alain

doi:10.4236/fns.2016.77063

Cited by 12 publications

(12 citation statements)

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“…Another hypothesis could be attributed to the presence of residual glucose from MRS culture allowing bifidobacteria to grow in medium exempt of carbohydrate. Indeed, the glucose present in initial MRS medium was in excess (Tanimomo et al, 2016 ), but a negligible part of it (maximum 1 mg) has been transferred with the inoculum. Another source of glucose could be the presence of residual carbohydrate in the yeast extracts or peptone extracts used in MRS media.…”

Section: Discussionmentioning

confidence: 99%

“…B. bifidum BBA1, a strain isolated from breastfed children feces, was chosen to provide a comparison with a strain of human origin as this strain should be able to use BMO or 3′SL as a source of carbon, due to their similarity with HMO (Zivkovic and Barile, 2011 ). Tanimomo et al ( 2016 ) developed a culture medium answering to the specificities of B. crudilactis FR/62/B/3. This formula, more suited to bifidobacteria from bovine origin, was used in this study as an optimized medium in which different sources of carbohydrates have been tested.…”

Section: Introductionmentioning

confidence: 99%

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Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Bondue¹,

Crèvecoeur²,

Brose³

et al. 2016

Front. Microbiol.

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Complex oligosaccharides from human milk (HMO) possess an antimicrobial activity and can promote the growth of bifidobacteria such as Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. Whey is rich in complex bovine milk oligosaccharides (BMO) structurally similar to HMO and B. crudilactis, a species of bovine origin, is able to metabolize some of those complex carbohydrates. This study focused on the ability of B. bifidum and B. crudilactis to grow in a culture medium supplemented in 3′-sialyllactose (3′SL) as the main source of carbon, a major BMO encountered in cow milk. Next, the effects of cell-free spent media (CFSM) were tested against virulence expression of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium. Both strains were able to grow in presence of 3′SL, but B. crudilactis showed the best growth (7.92 ± 0.3 log cfu/ml) compared to B. bifidum (6.84 ± 0.9 log cfu/ml). Then, CFSM were tested for their effects on virulence gene expression by ler and hilA promoter activity of luminescent mutants of E. coli and S. Typhimurium, respectively, and on wild type strains of E. coli O157:H7 and S. Typhimurium using RT-qPCR. All CFSM resulted in significant under expression of the ler and hilA genes for the luminescent mutants and ler (ratios of −15.4 and −8.1 respectively) and qseA (ratios of −2.1 and −3.1) for the wild type strain of E. coli O157:H7. The 3′SL, a major BMO, combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed in vitro will be further investigated regarding the overall phenotype of pathogenic agents and the exact nature of the active molecules.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Bondue¹,

Crèvecoeur²,

Brose³

et al. 2016

Front. Microbiol.

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“…Protective additives help to reduce cells damage during freeze‐drying (Tanimomo et al . ). Fermentation parameters also influence the cell, its freeze‐drying resistance and also the subsequent biocontrol efficacy.…”

Section: Introductionmentioning

confidence: 97%

Influence of fermentation temperature and duration on survival and biocontrol efficacy of Pseudomonas fluorescens Pf153 freeze‐dried cells

Bisutti

Stephan

2019

J Appl Microbiol

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Aim: The aim of this paper was to determine whether the quality of formulated Pseudomonas fluorescens Pf153 can be influenced by changes in fermentation conditions. In this study, the influence of the fermentation temperature on the growth and its consequence on shelf life, viability and biocontrol efficacy of freeze-dried cells of P. fluorescens Pf153 was investigated. Methods and Results: Cells of P. fluorescens Pf153 were grown at 20 and 28°C in flasks and fermenter and harvested in the mid-log and the beginning of the stationary phase. The survival during storage of freeze-dried cells was tested at 25°C. Cells fermented at 20°C survived in storage better than those grown at 28°C, irrespective of the harvesting time. Compared to the untreated control, in in vitro tests Pf153 was in all production temperature/duration combinations significantly effective against all tested Botrytis cinerea strains. But no differences between temperature/duration combinations were found. In bioassay on detached Vicia faba leaves, it was found that young cells, when fermented at 28°C had a significant positive influence on the biocontrol efficacy.Conclusions: These results demonstrate that fermentation parameters have an influence on the performance and quality of a formulated product. Significance and Impact of the Study: Only limited numbers of biocontrol products based on antagonistic pseudomonads are on the market. This can be attributed to the lack of suitable formulated products with high numbers of viable cells and a good shelf life. Currently, only limited information on the influence of the fermentation on subsequent downstreaming process is available. Within this study, we focused on the influence of the two important parameters fermentation temperature and harvest time on survival, shelf life and biocontrol efficacy of P. fluorescens Pf153.

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“…Four media with different carbohydrate sources were used: a medium without any glucose (MRS2 G), a reference medium with glucose (MRS2-G) [49], a medium with a mix of glucose and whey as a source of BMO (MRS2-Wh), and a medium with 3′SL (MRS2-3′SL) as the main source of carbohydrate (Table 1). The yeast extract, peptone of casein and glucose were provided by the Oxoid firm (Temse, Belgium).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Bifidobacterium mongoliense genome is particularly adapted to milk oligosaccharides digestion leading to production of antivirulent metabolites

Bondue

Milani

Arnould

et al. 2019

Preprint

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Human milk oligosaccharides (HMO) could promote the growth of bifidobacteria, improving young children’s health. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulent activity against intestinal pathogens. Bovine milk oligosaccharides (BMO), structurally similar to HMO, are found at high concentration in cow whey. This is particularly observed for 3’-sialyllactose (3’SL). This study focused on enzymes and transport systems involved in HMO/BMO metabolism contained in B. crudilactis and B. mongoliense genomes, two species from bovine milk origin. The ability of B. mongoliense to grow in media supplemented with whey or 3’SL was assessed. Next, the effects of cell-free spent media (CFSM) were tested against the virulence expression of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium. Due to the presence of genes encoding β-galactosidases, β-hexosaminidases, α-sialidases and α-fucosidases, B. mongoliense presents a genome more sophisticated and more adapted to the digestion of BMO/HMO than B. crudilactis (which contains only β-galactosidases). In addition, HMO/BMO digestion involves genes encoding oligosaccharide transport systems found in B. mongoliense but not in B. crudilactis. B. mongoliense seemed able to grow on media supplemented with whey or 3’SL as main source of carbon (8.3±1.0 and 6.7±0.3 log cfu/mL, respectively). CFSM obtained from whey resulted in a significant under-expression of ler, fliC, luxS, stx1 and qseA genes (-2.2, -5.3, -2.4, -2.5 and -4.8, respectively) of E. coli O157:H7. CFSM from 3’SL resulted in a significant up-regulation of luxS (2.0) gene and a down-regulation of fliC (-5.0) gene. CFSM obtained from whey resulted in significant up-regulations of sopD and hil genes (2.9 and 3.5, respectively) of S. Typhimurium, while CFSM obtained from 3’SL fermentation down-regulated hil and sopD genes (-2.7 and -4.2, respectively). From enzymes and transporters highlighted in the genome of B. mongoliense and its potential ability to metabolise 3’SL and whey, B. mongoliense seems well able to digest HMO/BMO. The exact nature of the metabolites contained in CFSM has to be identified still. These results suggest that BMO associated with B. mongoliense could be an interesting synbiotic formulation to maintain or restore intestinal health of young children.

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Growth and Freeze-Drying Optimization of <i>Bifidobacterium crudilactis</i>

Cited by 12 publications

References 25 publications

Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Influence of fermentation temperature and duration on survival and biocontrol efficacy of Pseudomonas fluorescens Pf153 freeze‐dried cells

Bifidobacterium mongoliense genome is particularly adapted to milk oligosaccharides digestion leading to production of antivirulent metabolites

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