Growth and glucosinolate profiles of Eruca sativa (Mill.) (rocket salad) and Diplotaxis tenuifolia (L.) DC. under different LED lighting regimes

Abstract

In this study, the growth and glucosinolate (GSL) profiles of rocket salad Eruca sativa (Mill.) and Diplotaxis tenuifolia (L.) DC. were determined during 30 days growing under different lighting regimes; T5_ peak at 545 nm, LED1_ peak at 631 nm and LED2_ peak at 598 nm. The biggest increase of dry weight (DW) was measured in E. sativa under T5 (0.657 g DW/plant) and the lowest in D. tenuifolia under LED1 (0.080 g DW/plant). GSL content was found to vary significantly, regardless of the light treatment, but it is related with genotype (E. sativa, r = 0.802**). On average, the highest amount of 4-methylsulfinylbutyl-GSL (glucosativin) (7.3248 mg/g DW) was quantified in E. sativa and D. tenuifolia (6.7428 mg/g DW) under the T5. The regression analysis between different light wavelengths and glucosinolates showed the strongest correlation between photosynthetic photon flux density (PPFD_B) and 4-methylthiobutyl-GSL (glucoerucin) in E. sativa (r = 0.698*) and D. tenuifolia (r = 0.693*), respectively, which indicates the effect of light on the response of plants to induced stress and changes in GSL biosynthesis.

In vitro application of Eruca vesicaria subsp. sativa leaf extracts and associated metabolites reduces the growth of Oomycota species involved in Kiwifruit Vine Decline Syndrome

“…chamaehyphon. GER was the second most abundant GSL compound quantified, as found in the literature (Stajnko et al, 2022), along with known anti-microbial activity (Kudjordjie et al, 2022). DGBT was the third most abundant, but there are no reports of its effectiveness against fungi in the literature.…”

“…As the use of soil fumigants is severely limited, the aim of our study was finding natural fungitoxic compounds as possible alternatives. Rocket was chosen as the species contains a high level of GSLs, related downstream hydrolysis products, and are fast-growing ( Stajnko et al., 2022 ). Thus, to better understand and discover further candidate biocontrol agents, the objective of our study was to determine the in vitro inhibitory effects of leaf extracts of several E. vesicaria cultivars towards the oomycetes strain, identify the GSLs content and the GSL-downstream products, and finally the effectiveness of pure compounds (ITCs).…”