Growth and integration of neocartilage with native cartilage in vitro

McCaffery, J. Michael; Spencer, Richard G.; Francomano, Clair A.

doi:10.1016/j.orthres.2004.08.028

Cited by 31 publications

(34 citation statements)

References 30 publications

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“…Costochondral cell neocartilage shows significant promise in replacing degenerated cartilage. However, challenges remain in establishing methods for stabilizing grafts and achieving integration in native cartilage [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Neocartilage integration in temporomandibular joint discs: physical and enzymatic methods

Murphy

Arzi

Prouty

et al. 2015

J. R. Soc. Interface.

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Integration of engineered musculoskeletal tissues with adjacent native tissues presents a significant challenge to the field. Specifically, the avascularity and low cellularity of cartilage elicit the need for additional efforts in improving integration of neocartilage within native cartilage. Self-assembled neocartilage holds significant potential in replacing degenerated cartilage, though its stabilization and integration in native cartilage require further efforts. Physical and enzymatic stabilization methods were investigated in an in vitro model for temporomandibular joint (TMJ) disc degeneration. First, in phase 1, suture, glue and press-fit constructs were compared in TMJ disc intermediate zone defects. In phase 1, suturing enhanced interfacial shear stiffness and strength immediately; after four weeks, a 15-fold increase in stiffness and a ninefold increase in strength persisted over press-fit. Neither suture nor glue significantly altered neocartilage properties. In phase 2, the effects of the enzymatic stabilization regimen composed of lysyl oxidase, CuSO 4 and hydroxylysine were investigated. A full factorial design was employed, carrying forward the best physical method from phase 1, suturing. Enzymatic stabilization significantly increased interfacial shear stiffness after eight weeks. Combined enzymatic stabilization and suturing led to a fourfold increase in shear stiffness and threefold increase in strength over press-fit. Histological analysis confirmed the presence of a collagen-rich interface. Enzymatic treatment additionally enhanced neocartilage mechanical properties, yielding a tensile modulus over 6 MPa and compressive instantaneous modulus over 1200 kPa at eight weeks. Suturing enhances stabilization of neocartilage, and enzymatic treatment enhances functional properties and integration of neocartilage in the TMJ disc. Methods developed here are applicable to other orthopaedic soft tissues, including knee meniscus and hyaline articular cartilage.

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Section: Introductionmentioning

confidence: 99%

“…Characteristics such as the low cellularity and avascularity of cartilage result in challenges in graft integration [6][7][8]26,27]. Towards enhancing the potential for neocartilage retention and integration, this work investigated physical and enzymatic stabilization methods in phases 1 and 2, respectively.…”

Section: Introductionmentioning

confidence: 99%

Neocartilage integration in temporomandibular joint discs: physical and enzymatic methods

Murphy

Arzi

Prouty

et al. 2015

J. R. Soc. Interface.

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“…After cells were dehydrated Pellet culture systems are established methods of culturing chondrocytes and mesenchymal stem cells for through graded concentrations of ethanol alone, a mixture of MNA (methyl nadic anhydride), Epon 812 Resin chondrogenesis (5,8,22,25,37,38). Lee et al (25) compared a new pellet culture system for human interverte-(TAAB, Berkshire, UK), DDSA (dodecenyl succinic anhydride), and DMP30 [2,4,6-tri (dimethylaminobral disc cells to a conventional alginate bead system in culture.…”

Section: Introductionmentioning

confidence: 99%

Periosteal Cell Pellet Culture System: A New Technique for Bone Engineering

et al. 2006

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To treat bone loss that is induced by disease or wounds, bone grafts are commonly used. In dentistry, guided tissue regeneration is effective in the treatment of periodontal diseases. However, bone resorption after implantation is a major problem with the bone graft and guided tissue regeneration technique. This study examines a cell pellet culture system without exogenous scaffolds for bone regeneration. First, we examined the effect of ascorbic acid on cells. Transmission electron microscopic observation revealed that cells formed a three-dimensional structure of multiple cell layers after 5 weeks of culturing in medium containing 50 µg/ ml ascorbic acid with the medium changed every 7 days. A single cell pellet was produced by centrifuging cells that were gathered from 10 tissue culture dishes. Van Gieson staining and collagen type I immunostaining showed that the pellet contained collagen fibers and cells that adhered to the collagen fibers. Several of these cell pellets were implanted subcutaneously on the backs of nude mice for 6 weeks. Histology and immunohistochemistry results indicated new bone formation, vascular invasion, and insular areas of calcification. Bone tissue was surrounded by osteoblasts. The appearance of new bone formation is similar to that seen in intramembranous ossification. The present pellet system is reliable and might solve problems of bone resorption after implantation.

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“…As a result, a rim of relatively acellular matrix surrounds the recipient site. This apoptotic response has been hypothesized to impede effective cartilage integration in cartilage repair procedures [50].…”

Section: Chondrocyte Apoptosis Associated With Osteochondral Graft Immentioning

confidence: 99%

“…However, some of this potential advantage is lost during the storage and implantation of these grafts due to chondrocyte cell death [5]. Furthermore, iatrogenic cell death at the recipient site is likely to have an adverse effect on edge integration of the donor cartilage [50].…”

Section: Introductionmentioning

confidence: 99%

Chondrocyte Apoptosis: Implications for Osteochondral Allograft Transplantation

Kim

Teng

Dang

2008

Clin Orthop Relat Res

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Osteochondral allograft transplantation is a useful technique to manage larger articular cartilage injuries. One factor that may compromise the effectiveness of this procedure is chondrocyte cell death that occurs during the storage, preparation, and implantation of the osteochondral grafts. Loss of viable chondrocytes may negatively affect osteochondral edge integration and longterm function. A better understanding of the mechanisms responsible for chondrocyte loss could lead to interventions designed to decrease cell death and improve results. Recent studies indicate that apoptosis, or programmed cell death, is responsible for much of the chondrocyte death associated with osteochondral allograft transplantation. Theoretically, some of these cells can be rescued by blocking important apoptotic mediators. We review the role of apoptosis in cartilage degeneration, focusing on apoptosis associated with osteochondral transplantation. We also review the pathways thought to be responsible for regulating chondrocyte apoptosis, as well as experiments testing inhibitors of the apoptotic pathway. These data suggest that key contributors to the apoptotic process can be manipulated to enhance chondrocyte survival. This knowledge may lead to better surgical outcomes for osteochondral transplantation.

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Growth and integration of neocartilage with native cartilage in vitro

Cited by 31 publications

References 30 publications

Neocartilage integration in temporomandibular joint discs: physical and enzymatic methods

Neocartilage integration in temporomandibular joint discs: physical and enzymatic methods

Periosteal Cell Pellet Culture System: A New Technique for Bone Engineering

Chondrocyte Apoptosis: Implications for Osteochondral Allograft Transplantation

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