Growth and intrinsic labeling of peanuts with copper-65 for use in human bioavailibility studies

Starks, Thomas L.; Johnson, Phyllis E.

doi:10.1021/jf00064a055

Cited by 6 publications

(1 citation statement)

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“…Intrinsic labeling of nutrients in plants is a key technique for studying bioavailability so that recently ingested nutrients may be discerned from endogenous nutrients in biological samples. A variety of techniques have been used for labeling minerals in plants, including enrichment of nutrient solution ( − ), stem injection ( − ), and foliar application ( , ). For labeling of organics in plants for bioavailability studies, deuterium oxide enrichment of the nutrient solution has been investigated by Grusak ( , ).…”

Section: Discussionmentioning

confidence: 99%

Isotopic Labeling and LC-APCI-MS Quantification for Investigating Absorption of Carotenoids and Phylloquinone from Kale (Brassica oleracea)

Kurilich

Britz

Clevidence

et al. 2003

J. Agric. Food Chem.

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The ability to study bioavailability of nutrients from foods is an important step in determining the health impact of those nutrients. This work describes a method for studying the bioavailability of nutrients from kale (Brassica oleracea var. Acephala) by labeling the nutrients with carbon-13, feeding the kale to an adult volunteer, and analyzing plasma samples for labeled nutrients. Results showed that conditions for producing atmospheric intrinsically labeled kale had no detrimental effect on plant growth. Lutein, beta-carotene, retinol, and phylloquinone were analyzed using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. Analysis of plasma samples showed that labeled lutein peaked in plasma at 11 h (0.23 microM), beta-carotene peaked at 8 (0.058 microM) and 24 h (0.062 microM), retinol peaked at 24 h (0.10 microM), and phylloquinone peaked at 7 h (3.0 nM). This method of labeling kale with (13)C was successful for producing clearly defined kinetic curves for (13)C-lutein,(13)C-beta-carotene, (13)C-retinol, and (13)C-phylloquinone.

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