Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures

Abstract

We established cell suspension cultures derived from leaf, stem, and root calli of Pueraria candollei var. candollei and P. candollei var. mirifica using liquid Murashige and Skoog (MS) medium supplemented with 0.56 lM 6-benzyladenine (BA) and 4.52 lM 2,4-dichlorophenoxyacetic acid (2,4-D). Growth of the cell suspension cultures progressed to the stationary phase within 15-24 days. Methanolic extracts of cell suspension cultures of both varieties of P. candollei were analyzed using a validated HPLC protocol. All cell lines derived from leaf, stem, and root explants produced four major isoflavonoids: daidzein, daidzin, genistein, and genistin; these isoflavonoids were detected only in the roots of intact plants. Furthermore, the isoflavonoid contents of the cell suspension cultures were higher than those of intact plants. Thus, cell suspension culture of both varieties of P. candollei may be an effective tool for isoflavonoid production.

Salicylic acid on antioxidant activity and betacyan in production from leaves of Alternanthera tenella

“…Salicylic acid affects the secondary metabolism of plants (BOONSNONGCHEEP et al, 2010;KORSANGRUANG et al, 2010) and has been widely studied in relation to signaling mechanisms and responses to pests and diseases (FUJITA et al, 2006). In the present study, exposing A. tenella to 400µM salicylic acid signifi cantly infl uenced the betacyanin content of the leaves.…”

Isoflavones and biotransformed dihydrodaidzein in hairy roots of Korean wild arrowroot

“…Although many results in the growth curves of cell suspension cultures with Pueraria species have been reported (Boonsnongcheep et al 2010;Chen and Li 2007;, those of hairy root cultures were rare. Therefore, the growth characteristics of the hairy roots were examined during the 4 weeks of culture using the fresh and dry weights.…”

“…The pathway before daidzein suggested that puerarin and daidzin were converted to daidzein (Jin et al 2008;Park et al 2006). In previous studies, the possibility of the enhanced accumulation of isoflavones has been reported in cell suspension cultures (Boonsnongcheep et al 2010;Korsangruang et al 2010;Lee et al 2013;Vaishnav et al 2006). On the other hand, there are only a few reports on hairy root cultures, despite isoflavone production by hairy root cultures being studied in a Psoralea species and other Pueraria species (Bourgaud et al 1999;Udomsuk et al 2011).…”

Isoflavones and biotransformed dihydrodaidzein production with in vitro cultured callus of Korean wild arrowroot Pueraria lobata

“…앞서 언급한 바와 같이 칡은 기능적으로 많은 효과가 있는 것으로 알려져 있으나, 뿌리 부위에 가장 많은 성분 이 포함되어 있어 (Kim et al 2004) 이를 이용하기 위하여 야생의 칡을 수거하는데 큰 어려움이 있다. 따라서 본 연 구에서는 타 Pueraria 속 식물을 대상으로 제한적으로 진 행되었던 캘러스와 모상근의 유도 및 배양을 통해 생산된 재료에서의 이소플라본 생성 검증 연구 (Vaishnav et al 2006;Reppert et al 2008;Boonsnongcheep et al 2010;Korsangruang et al 2010;Udomsuk et al 2011 …”