Growth and metastasis of human tumors in nude mice following tumor‐cell inoculation into a vascularized polyurethane sponge matrix

Abstract

Growth and metastasis of human tumor cells in immunodeficient nude mice were improved when tumor cells were inoculated within a vascularized artificial polyurethane sponge matrix. The sponges had been implanted 7-10 days earlier and were vascularized at the time of cell injection. All cell lines tested, including colon carcinoma-derived lines from primary tumors (HT29, PT3 and PT4) or from liver metastasis (LM3), and a metastatic variant from a melanoma (MeWo-Met) grew in a high percentage (78-94%) of the inoculated sponge grafts. When growth in sponge grafts is compared with growth at a subcutaneous site, the sponge matrix appears to increase tumorigenicity, at least for some cell lines. Regular formation of metastases was observed when cells had been injected into sponges. Most metastases were found in a second sponge graft implanted at a contralateral site, but some were also found at other s.c. sites. In vivo depletion of NK cells by pre-treatment with cyclophosphamide could not further enhance the formation of metastasis. Tumor cells from fresh surgical specimens could be propagated in sponge matrix grafts and subsequently established as cell lines in tissue culture.

Sequential Induction of Effector Function, Tissue Migration and Cell Death during Polyclonal Activation of Mouse Regulatory T-Cells

“…Polyester-polyurethane sponges (5×5 mm 2 ) (Vitaform Ltd, Manchester, UK) containing heatkilled Listeria monocytogenes were implanted s.c. in C57BL/6 mice [44]. After 4 days mice were treated with 200 µg CD28SA or PBS i.p.. On day 7 sponges were recovered, cells were washed out several times with BSS/BSA and stained for Foxp3 and IL-10 via IL-10 Secretion Assay.…”

In vivo models of angiogenesis

“…The growth and metastasis of human tumors in nude mice following tumor-cell inoculation into a vascularized polyurethane sponge matrix have been studied [97]. The sponge material induces a nonspecific inflammatory reaction, which mediates angiogenesis and connective tissue infiltration.…”

Expression of Endothelial Cell Adhesion Molecules in Neovascularized Tissue

“…Sponge implants have been previously employed for in vivo characterization of different facets of the function of newly formed or developing blood vessels, including their pharmacological reactivity (3), role in tumor growth and metastasis (43), and responsiveness to angiogenic agents (2). Recently, it has been shown that several angiogenic peptides, including VEGF and bFGF, can interfere with the expression of endothelial glycoproteins on proliferating endothelial cells (18,31).…”