Growth and mitotic chromosomes of soybean (<i>Glycine max L. Merril</i>) plants as affected by spent engine oil in sandy-loam soils

Abstract

Soybean (Glycine max L. Merril) is one of the most nutritionally valuable legumes in Africa. However, spent automobile engine oil is always disposed indiscriminately on lands and water bodies in Nigeria, which pollutes both terrestrial and aquatic ecosystems, with adverse effects on crop growth and productivity. This study investigated the effects of spent engine oil (SEO) polluted soils on growth parameters and mitotic chromosomes of soybean. Two soybean varieties (TGX-1448-2E and local) were planted in pots filled with sandy-loam soil treated with 0, 0.1, 0.4 and 0.7% v/w SEO concentrations, in 2 × 4 factorial experiment laid in a completely randomized design with six replications. Data were collected at weekly intervals for four weeks on growth parameters (plant height, number of leaves, leaf width and leaf length) and chromosomal aberration. Data were subjected to ANOVA and means separated with LSD. Among the treatments, seed germination rate was significantly higher in TGX-1448-2E (95.0 ± 0.0-96.0 ± 0.7%) than local variety (70.0 ± 0.7-71.0 ± 0.7%). Both 0.4 and 0.7% SEO caused significant (p ≤ 0.01) reductions in plant height, number of leaves, leaf length and leaf width whereas, 0.1% did not reduce growth parameters. Treatments 0.4 and 0.7% SEO caused high chromosomal aberrations in soybean as they reduced chromosome numbers (from 40.0 to 23%), cell division, mitotic index (70.0 to 20.8%) and produced 31.7% abnormal cells. Abnormalities such as bridges, laggards and chromosome stickiness occurred. This study shows that 0.4-0.7% SEO polluted soils is phytotoxic to soybeans.

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

“…4 c). Stickiness results from either enhanced chromosomal contraction or condensation or from DNA depolymerization and partial breakdown of nucleoproteins 75 . It is a specific type of chromosomal abnormality that prevents mitotic division by binding together several chromosomes 76 .…”

“…Hussein 77 pointed out MN induction and stickiness in A. cepa as the most prominent indicators of cytotoxicity. The toxic consequences of sticky chromosomes are typically permanent and may cause cellular death 75 . In this case, it can be said that Mn not only suppresses cell division but also has the potential to kill cells.…”