Growth and morphogenesis of shoot initials of Douglas fir, Pseudotsuga menziesii (Mirb.) Franco, in vitro.

Evers, P.W.

doi:10.18174/njas.v33i2.16865

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…For instance, the higher parts of donor plant are older than the lower parts. Evers [28] has reported that shoot initials from lower parts of Pseudotsuga menziesii developed better under in vitro conditions. Explants excised from the base produce adventitious bulbs more easily than the top parts of the bulbs [3].…”

Section: Explant Position In Donor Plantmentioning

confidence: 99%

The Prerequisite of the Success in Plant Tissue Culture: High Frequency Shoot Regeneration

Yıldız¹

2012

Recent Advances in Plant in Vitro Culture

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Providing infection-free plants for mass production is possible. Plant tissue culture is based on totipotency which means that a whole plant can be regenerated from a single cell on a growth medium. One of the main objectives of tissue culture studies is to obtain high-frequency shoot regeneration, which is also a prerequisite for an efficient transformation system and a clonal propagation of plants with attractive flowers and fruits in large scale for ornamental purposes. Specially the introduction of foreign genes coding agronomically important traits into plant cells has no meaning unless transgenic plants are regenerated from the genetically modified cell(s). It is known that some families and genera such as Solanacea (Nicotiana, Petunia and Datura), Cruciferae (Brassica and Arabidopsis), Gesneriaceae (Achimenes and Streptocarpus), Asteraceae (Chichorium and Chrysanthemum) and Liliaceae (Lilium and Allium) have a high regeneration capacity while regeneration in some other families such as Malvaceae (Gossypium) and Chenopodiaceae (Beta) is difficult. In order to increase the regeneration capacity of explant from the genotype of interest, we have to find answers to such questions as "Why do some genotypes regenerate easily?", "What can be done to increase the regeneration capacity of explant?". 2. Factors affecting explant's regeneration capacity 2.1. Plant material Plant material is extremely important for the success of tissue culture studies [4]. Factors affecting explant's tissue culture response are (1) genotype, (2) physiological stage of donor plant, (3) explant source, (4) explant age, (5) explant size, (6) explant position in donor plant and (7) explant density. Plant segments used in tissue culture as explant are stem [5], root [6], leaf [7], flower [8], ovule [9], cotyledon and hypocotyl [10, 11]. Such these explants form direct and indirect organs and embriyos. Thin cell layer can also be used as explant in some species [12] while embryos can be successfully used in cereals [13]. Moreover, shoot tips and meristems may give successful results for callus formation and shoot regeneration [14]. 2.1.1. Genotype Regeneration capacity of plants shows a wide range among families, species and even within genotypes from the same species (Figure 1). Generally dicotyledons regenerate more easily than monocots. Plants from some dicotyledon families such as Solanacea, Cruciferae, Gesneriaceae, Begoniaceae and Crassulaceae have a high regeneration capacity. In general, herbaceous plants regenerate more easily than woody plants such as trees and shrubs [3]. Sugarbeet from Chenopodiaceae family is known as a recalcitrant genotype with respect to in vitro culture and genetic transformation [15, 16] (Figure 2) while regeneration and transformation are quite easy in tobacco from Solanaceae family [17]. It was reported that somatic embryogenesis changed from 0.00% to 77.50% in 14 maize genotypes cultured in vitro [18].

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Section: Explant Position In Donor Plantmentioning

confidence: 99%

The Prerequisite of the Success in Plant Tissue Culture: High Frequency Shoot Regeneration

Yıldız¹

2012

Recent Advances in Plant in Vitro Culture

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“…Nos résultats sont à rapprocher de ceux obtenus par Boulay [13] qui, lors de l'étude de l'élongation de la tige de Pseudotsuga menziesii, a montré que le charbon actif empêchait l'arrêt de croissance ; un tel effet pourrait être dû à l'action stimulante des impuretés (monophénylamines) du charbon actif. Dans le même sens, Evers [14] a montré que l'adjonction de charbon actif dans un milieu de culture avait souvent un effet sur l'organogenèse des espèces ligneuses. Il en est de même pour Picea [15] et pour Psidium guajava [16] qui ont présenté une même réponse positive en présence du charbon actif.…”

Section: Discussionunclassified

Régénérationin vitrode plants à partir des bourgeons axillaires et de l'apex de plantules sexuées deDacryodes edulis(Don) Lam.

Youmbi

Benbadis

2001

Fruits

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Effects of the Physical Environment

George¹,

Hall

Klerk³

Plant Propagation by Tissue Culture

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Growth and morphogenesis of shoot initials of Douglas fir, Pseudotsuga menziesii (Mirb.) Franco, in vitro.

Cited by 5 publications

References 4 publications

The Prerequisite of the Success in Plant Tissue Culture: High Frequency Shoot Regeneration

The Prerequisite of the Success in Plant Tissue Culture: High Frequency Shoot Regeneration

Régénérationin vitrode plants à partir des bourgeons axillaires et de l'apex de plantules sexuées deDacryodes edulis(Don) Lam.

Effects of the Physical Environment

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