Growth and mycotoxin production by Aspergillus fumigatus strains isolated from a saltern

Tepšič, K.; Gunde‐Cimerman, Nina; Frisvad, Jens Christian

doi:10.1111/j.1574-6968.1997.tb12745.x

Cited by 33 publications

(5 citation statements)

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“…Pseurotin A is a competitive inhibitor of chitin synthase and has been reported to have cytotoxic activity, thereby highlighting its potentially cytotoxic effect on host cells [64][65][66]. Verruculogen and fumitremorgin b are known to affect the central nervous system in vivo and in vitro; verruculogen alters the electrophysiological properties of human nasal epithelial cells, suggesting this metabolite may participate in fungal colonization of the airways [67,68]. Fumagillin affects the ability of neutrophils to produce superoxide anions by inhibiting the function of NADPH oxidase and prevents degranulation by interfering with the structural rearrangements required for these processes to occur [69].…”

Section: Discussionmentioning

confidence: 99%

Exposure to the Pseudomonas aeruginosa secretome alters the proteome and secondary metabolite production of Aspergillus fumigatus

et al. 2022

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The fungal pathogen Aspergillus fumigatus is frequently cultured from the sputum of cystic fibrosis (CF) patients along with the bacterium Pseudomonas aeruginosa. A. fumigatus secretes a range of secondary metabolites, and one of these, gliotoxin, has inhibitory effects on the host immune response. The effect of P. aeruginosa culture filtrate (CuF) on fungal growth and gliotoxin production was investigated. Exposure of A. fumigatus hyphae to P. aeruginosa cells induced increased production of gliotoxin and a decrease in fungal growth. In contrast, exposure of A. fumigatus hyphae to P. aeruginosa CuF led to increased growth and decreased gliotoxin production. Quantitative proteomic analysis was used to characterize the proteomic response of A. fumigatus upon exposure to P. aeruginosa CuF. Changes in the profile of proteins involved in secondary metabolite biosynthesis (e.g. gliotoxin, fumagillin, pseurotin A), and changes to the abundance of proteins involved in oxidative stress (e.g. formate dehydrogenase) and detoxification (e.g. thioredoxin reductase) were observed, indicating that the bacterial secretome had a profound effect on the fungal proteome. Alterations in the abundance of proteins involved in detoxification and oxidative stress highlight the ability of A. fumigatus to differentially regulate protein synthesis in response to environmental stresses imposed by competitors such as P. aeruginosa . Such responses may ultimately have serious detrimental effects on the host.

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Section: Discussionmentioning

confidence: 99%

Exposure to the Pseudomonas aeruginosa secretome alters the proteome and secondary metabolite production of Aspergillus fumigatus

et al. 2022

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“…Janso et al [ 98 ] explored the effect of media with 5, 10, 15 or 20% NaCl on the growth of Penicillium dravuni with the metabolites dityosphaeric acids A and B and carviolin produced in fermentation with 50% artificial seawater. Tepšič et al [ 99 ] isolated Aspergillus fumigatus strains from salty soil, with salt concentration from 0.5–1 M NaCl and examined their potential to produce secondary metabolites. The production of secondary metabolites was much reduced and none of the mycotoxins investigated (verruculogen, fumitremorgins, fumagillin) were detected in media with water activity below 0.878 [ 99 ].…”

Section: Salinity Effect On Production Of Bioactive Compounds and Oth...mentioning

confidence: 99%

“…Tepšič et al [ 99 ] isolated Aspergillus fumigatus strains from salty soil, with salt concentration from 0.5–1 M NaCl and examined their potential to produce secondary metabolites. The production of secondary metabolites was much reduced and none of the mycotoxins investigated (verruculogen, fumitremorgins, fumagillin) were detected in media with water activity below 0.878 [ 99 ]. Overy et al [ 100 ] also investigated the effect of salinity stress on the expression of secondary metabolite production in the terrestrial fungus Aspergillus aculeatus .…”

Section: Salinity Effect On Production Of Bioactive Compounds and Oth...mentioning

confidence: 99%

How Do Fungi Survive in the Sea and Respond to Climate Change?

Jones

Ramakrishna

Sabaratnam

et al. 2022

JoF

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With the over 2000 marine fungi and fungal-like organisms documented so far, some have adapted fully to life in the sea, while some have the ability to tolerate environmental conditions in the marine milieu. These organisms have evolved various mechanisms for growth in the marine environment, especially against salinity gradients. This review highlights the response of marine fungi, fungal-like organisms and terrestrial fungi (for comparison) towards salinity variations in terms of their growth, spore germination, sporulation, physiology, and genetic adaptability. Marine, freshwater and terrestrial fungi and fungal-like organisms vary greatly in their response to salinity. Generally, terrestrial and freshwater fungi grow, germinate and sporulate better at lower salinities, while marine fungi do so over a wide range of salinities. Zoosporic fungal-like organisms are more sensitive to salinity than true fungi, especially Ascomycota and Basidiomycota. Labyrinthulomycota and marine Oomycota are more salinity tolerant than saprolegniaceous organisms in terms of growth and reproduction. Wide adaptability to saline conditions in marine or marine-related habitats requires mechanisms for maintaining accumulation of ions in the vacuoles, the exclusion of high levels of sodium chloride, the maintenance of turgor in the mycelium, optimal growth at alkaline pH, a broad temperature growth range from polar to tropical waters, and growth at depths and often under anoxic conditions, and these properties may allow marine fungi to positively respond to the challenges that climate change will bring. Other related topics will also be discussed in this article, such as the effect of salinity on secondary metabolite production by marine fungi, their evolution in the sea, and marine endophytes.

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“…In addition, the mentioned previous studies may be linked to the observations of the current study, which indicate low expression levels of HSP70 at temperatures below normal mammalian and human body temperature. Furthermore, previous observations may indicate that A. fumigatus, a frequently isolated filamentous fungus from extreme environments (49), may be adapted to ambient temperatures rather than physiological temperatures and host body conditions (43). This means that the environmental conditions in A. fumigatus induce a normal signal while host niches induce a stressed or abnormal signal to regulate HSPs.…”

Section: Culture Temperature (˚C) -----------------------------------mentioning

confidence: 99%

A comparative study of the timecourse of the expression of the thermo-inducible HSP70 gene in clinical and environmental isolates of Aspergillus fumigatus

Sharafi

Khosravi

Vahedi

et al. 2016

Molecular Medicine Reports

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The internal environment within animals or humans provides different conditions to invading saprophytic fungal pathogens, requiring the differential regulation of genes in comparison to environmental conditions. Understanding the mechanisms by which pathogens regulate genes within the host may be key in determining pathogen behavior within the host and may additionally facilitate further investigation into novel therapeutic agents. The heat shock protein (HSP)70 gene and its associated proteins have been frequently reported to be among the most highly expressed and dominant proteins present within various locations at physiological temperatures. The present study examined relative gene expression levels of the HSP70 gene in Aspergillus fumigatus isolates from both clinical and environmental origins, at a range of temperature points (20, 30, 37 and 42˚C) over five days, using reverse transcription‑quantitative polymerase chain reaction, comparing with a standard A. fumigatus strain incubated at 25˚C. The results indicated a differential gene expression pattern for the environmental and clinical isolates. During the five days, the HSP70 expression levels in the clinical samples were higher than in the environmental samples. However, the difference in the expression levels between the two groups at 42˚C was reduced. The mean HSP70 expression level over the five incubation days demonstrated a gradual and continual increasing trend by temperature elevation in both groups at 30, 37 and 42˚C, however, at 20˚C both groups demonstrated reduced expression. The temperature shift from 20 to 42˚C resulted in HSP70 induction and up to a 10‑ and 8.6‑fold change in HSP70 expression levels on the fifth day of incubation in the clinical and environmental groups, respectively. In conclusion, incubation at 37 and 42˚C resulted in the highest expression levels in both experimental groups, with these temperature points important for the induction of HSP70 expression in A. fumigatus.

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Growth and mycotoxin production by Aspergillus fumigatus strains isolated from a saltern

Cited by 33 publications

References 5 publications

Exposure to the Pseudomonas aeruginosa secretome alters the proteome and secondary metabolite production of Aspergillus fumigatus

Exposure to the Pseudomonas aeruginosa secretome alters the proteome and secondary metabolite production of Aspergillus fumigatus

How Do Fungi Survive in the Sea and Respond to Climate Change?

A comparative study of the timecourse of the expression of the thermo-inducible HSP70 gene in clinical and environmental isolates of Aspergillus fumigatus

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