Growth and shape transformations of giant phospholipid vesicles upon interaction with an aqueous oleic acid suspension

Peterlin, Primož; Arrigler, Vesna; Kogej, Ksenija; Svetina, S.; Walde, Peter

doi:10.1016/j.chemphyslip.2009.03.005

Cited by 87 publications

(93 citation statements)

References 58 publications

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“…However, experiments with phospholipid GUVs revealed that oleic acid addition produce vesicles attached to the parent GUV by thin membrane bridges (Peterlin et al, 2009). These findings indicate that in phospholipid systems the non-local forces producing membrane budding are not sufficient to break the final membrane connection.…”

Section: Fission and Geometric Constraintsmentioning

confidence: 96%

Geometry of membrane fission

Frolov

Escalada

Akimov

et al. 2015

Chemistry and Physics of Lipids

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Section: Fission and Geometric Constraintsmentioning

confidence: 96%

Geometry of membrane fission

Frolov

Escalada

Akimov

et al. 2015

Chemistry and Physics of Lipids

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“…Division events were not observed in control experiments lacking copper. Based on previous studies, we speculate that the observed vesicle morphological changes can be explained by in situ phospholipid synthesis, which drives an increase in the vesicle surface area-to-volume ratio (29,(38)(39)(40)(41)(42). To compensate for the increased membrane material and relieve curvature stress, the vesicles either adopt nonspherical shapes or spontaneously divide.…”

Section: Significancementioning

confidence: 99%

Self-reproducing catalyst drives repeated phospholipid synthesis and membrane growth

Hardy

Yang

Selimkhanov

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

153

150

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Cell membranes are dynamic structures found in all living organisms. There have been numerous constructs that model phospholipid membranes. However, unlike natural membranes, these biomimetic systems cannot sustain growth owing to an inability to replenish phospholipid-synthesizing catalysts. Here we report on the design and synthesis of artificial membranes embedded with synthetic, self-reproducing catalysts capable of perpetuating phospholipid bilayer formation. Replacing the complex biochemical pathways used in nature with an autocatalyst that also drives lipid synthesis leads to the continual formation of triazole phospholipids and membrane-bound oligotriazole catalysts from simpler starting materials. In addition to continual phospholipid synthesis and vesicle growth, the synthetic membranes are capable of remodeling their physical composition in response to changes in the environment by preferentially incorporating specific precursors. These results demonstrate that complex membranes capable of indefinite self-synthesis can emerge when supplied with simpler chemical building blocks.membranes | autocatalysis | self-assembly | lipids

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“…Transfer and embedment of OA into the cellular membrane would lead to its destabilization and, for large doses, their complete disruption. Using existing frameworks for understanding OA effect on membranes26 , we propose an explanation for how the protein-component of PFA complexes can be detected in the cytosol and many other places in the cell without invoking active…”

mentioning

confidence: 99%

α-Lactalbumin:Oleic Acid Complex Spontaneously Delivers Oleic Acid to Artificial and Erythrocyte Membranes

Wen

Strømland

Halskau

2015

Journal of Molecular Biology

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Human α-lactalbumin made lethal to tumor cells (HAMLET) is a tumoricidal complex consisting of human α-lactalbumin and multiple oleic acids (OAs). OA has been shown to play a key role in the activity of HAMLET and its related complexes, generally known as protein-fatty acid (PFA) complexes. In contrast to what is known about the fate of the protein component of such complexes, information about what happens to OA during their action is still lacking. We monitored the membrane, OA and protein components of bovine α-lactalbumin complexed with OA (BLAOA; a HAMLET-like substance) and how they associate with each other. Using ultracentrifugation, we found that the OA and lipid components follow each other closely. We then firmly identify a transfer of OA from BLAOA to both artificial and erythrocyte membranes, indicating that natural cells respond similarly to BLAOA treatment as artificial membranes. Uncomplexed OA is unable to similarly affect membranes at the conditions tested, even at elevated concentrations. Thus, BLAOA can spontaneously transfer OA to a lipid membrane. After the interaction with the membrane, the protein is likely to have lost most or all of its OA. We suggest a mechanism for passive import of mainly uncomplexed protein into cells, using existing models for OA's effect on membranes. Our results are consistent with a membrane destabilization mediated predominantly by OA insertion being a significant contribution to PFA cytotoxicity.

show abstract

Growth and shape transformations of giant phospholipid vesicles upon interaction with an aqueous oleic acid suspension

Cited by 87 publications

References 58 publications

Geometry of membrane fission

Geometry of membrane fission

Self-reproducing catalyst drives repeated phospholipid synthesis and membrane growth

α-Lactalbumin:Oleic Acid Complex Spontaneously Delivers Oleic Acid to Artificial and Erythrocyte Membranes

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