Growth‐ and stress‐related defects associated with wall hypoacetylation are strigolactone‐dependent

Ramírez, Vicente; Xiong, Guangyan; Mashiguchi, Kiyoshi; Yamaguchi, Shinjiro; Pauly, Markus

doi:10.1002/pld3.62

Cited by 23 publications

(20 citation statements)

References 63 publications

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“…Double mutants were generated by crossing and identified by genotyping of individuals in the F2 generation. Genotyping of T‐DNA lines was performed as described in Ramírez et al., (see for primer sequences). To genotype max2‐1 mutation, the amplicon generated by PCR using max2‐1F/max2‐1 R primer combination was digested with ApoI restriction enzyme resulting in a 147 bp single fragment in wildtype plants and two fragments (76 and71 bp) in mutant plants.…”

Section: Methodsmentioning

confidence: 99%

“…Xylem morphology was evaluated as described in Ramírez et al. (). Equivalent segments of plant stems from the various genotypes were used to prepare hand‐cut sections.…”

Section: Methodsmentioning

confidence: 99%

“…Total destarched alcohol‐insoluble residue was prepared and cellulose content, matrix polysaccharide composition, and total wall acetate were determined according to Foster, Martin, and Pauly () as described in Ramírez et al. ().…”

Section: Methodsmentioning

confidence: 99%

“…How KAK, a putative ubiquitin E3 ligase involved in endoreduplication and control of DNA content in trichomes, regulates xylem development remains elusive (Bensussan et al., ; Downes, Stupar, Gingerich, & Vierstra, ; El Refy et al., ). In another case, mutations in MAX4 similarly rescue tbl29 collapsed xylem, dwarfism, and increased freezing tolerance (Ramírez, Xiong, Mashiguchi, Yamaguchi, & Pauly, ). MAX4 encodes a key enzyme in SL biosynthesis (Sorefan et al., ), suggesting that the tbl29 ‐triggered irx syndrome is SL‐dependent.…”

Section: Introductionmentioning

confidence: 98%

“…This requirement was further confirmed by the chemical complementation assays using a synthetic SL (i.e., GR‐24). tbl29 max4 double mutant plants growing in the presence of GR‐24 exhibit collapsed xylem and dwarfed stature, indicating that complementing the SL deficiency with exogenous applications of SL circumvent the max4 effect (Ramírez et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis

Ramírez

Pauly

2019

Plant Direct

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Defects in the biosynthesis and/or deposition of secondary plant cell wall polymers result in the collapse of xylem vessels causing a dwarfed plant stature and an altered plant architecture termed irregular xylem ( irx ) syndrome. For example, reduced xylan O ‐acetylation causes strong developmental defects and increased freezing tolerance. Recently, we demonstrated that the irx syndrome in the trichome birefringence‐like 29/eskimo1 ( tbl29/esk1 ) mutant is dependent on MORE AXILLARY GROWTH 4 ( MAX 4), a key enzyme in the biosynthesis of the phytohormone strigolactone ( SL ). In this report, we show that other xylan‐ and cellulose‐deficient secondary wall mutants exhibit increased freezing tolerance correlated with the irx syndrome. In addition, these phenotypes are also dependent on MAX 4, suggesting a more general interaction between secondary wall defects and SL biosynthesis. In contrast, MAX 4 does not play a role in developmental defects triggered by primary wall deficiencies, suggesting that the interaction is restricted to vascular tissue. Through a reverse genetics approach, the requirement of different components of the SL pathway impacting the irx syndrome in tbl29 was evaluated. Our results show that the tbl29 ‐associated irx phenotypes are dependent on the MAX 3 and MAX 4 enzymes, involved in the early steps of SL biosynthesis. In contrast, this signaling is independent on downstream enzymes in the biosynthesis and perception of SL such as MAX 1 and MAX 2.

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Section: Methodsmentioning

confidence: 99%

“…Xylem morphology was evaluated as described in Ramírez et al. (). Equivalent segments of plant stems from the various genotypes were used to prepare hand‐cut sections.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis

Ramírez

Pauly

2019

Plant Direct

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Effects of ensemble‐forecasted key environmental factors on the distribution, active constituents, and transcription regulation in Ligusticum chuanxiong Hort

Zhang,

Zhou,

Han

et al. 2024

J Sci Food Agric

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BACKGROUNDLigusticum chuanxiong Hort., with over 2000 years of medicinal use and cultivation history, is extensively used in clinical settings for treating heart disease, headache, dysmenorrhea, and amenorrhea. Constructing the geographic distribution pattern of L. chuanxiong and identifying the environmental factors limiting its range, as well as clarifying the effects of key environmental factors on the content of major active constituents and transcription regulation, could provide a scientific foundation for the conservation and effective management of this valuable medicinal resource.RESULTSThe results reveal that the predominant environmental factors influencing the distribution were the minimum temperature of the coldest month (Bio6) and solar radiation (Srad), with cumulative account for 87.46% of the importance. Correlation analysis further reveals significant negative correlations between Bio6 and the content of major active constituents in L. chuanxiong, with Srad exhibiting a negative correlation with these constituents. The gene differential expression analysis indicated that the expression levels of some genes associated with growth and active constituent biosynthesis pathways, such as RPT2_13888, UVR8_16871, CLPB3_3155, and 4CLL5_116, varied significantly among locations influenced by differing key environmental factors. Consequently, alterations in the environment were found to influence the gene expression levels within these pathways, resulting in variations in the content of active constituents.CONCLUSIONSThese findings contribute to an enhanced understanding of how environmental factors impact the distribution and quality of medicinal plants and offer a theoretical reference for the introduction, cultivation, quality improvement, resource utilization and management of L. chuanxiong. © 2024 Society of Chemical Industry.

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Strigolactones: A Novel Carotenoid-Derived Phytohormone – Biosynthesis, Transporters, Signalling, and Mechanisms in Abiotic Stress

Hossain

Raza

Maitra

et al. 2021

Plant Growth Regulators

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Growth‐ and stress‐related defects associated with wall hypoacetylation are strigolactone‐dependent

Cited by 23 publications

References 63 publications

Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis

Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis

Effects of ensemble‐forecasted key environmental factors on the distribution, active constituents, and transcription regulation in Ligusticum chuanxiong Hort

Strigolactones: A Novel Carotenoid-Derived Phytohormone – Biosynthesis, Transporters, Signalling, and Mechanisms in Abiotic Stress

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