Growth and survival of Bifidobacterium breve and Bifidobacterium longum in various sugar systems with fructooligosaccharide supplementation

Abstract

This study aims to investigate the effect of fructooligosaccharide (FOS) (0.5, 1, 2, 3, and 4%) supplementation on the growth and survival of Bifidobacterium breve and Bifidobacterium longum in glucose, fructose, lactose, and sucrose (2, 3, and 4%) systems with 24-h growth and 10-day survival assays at 37 °C. FOS supplementation showed a higher growth-promoting effect on B. longum than B. breve in various sugar systems. The highest percentage of increase in growth index, 78.5%, was observed with 4% sucrose supplemented with 0.5% FOS in B. longum. In comparison, the highest percentage increase in growth index, 5.6 and 6.6%, was observed in the presence of 2% glucose and 4% lactose supplemented with 0.5% FOS in B. breve. In survival assay, FOS supplementation (0.5–4%) in a 2% lactose system showed the highest positive effect on the cell viability of B. longum on day-10. As for B. breve, FOS supplementation (1 and 2%) in the 2% sucrose system showed the highest positive effect on the cell viability, followed by FOS supplementation (0.5, 3, and 4%) in 2% sucrose and FOS supplementation (3 and 4%) in 2% lactose on day-10. This study demonstrated that the efficacy of FOS supplementation was depended on its concentration, sugar system and its concentration, and Bifidobacterium strain.

Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization

“…In this study, we found that 9.51% of COG, are involved in metabolizing and transporting carbohydrates. In addition to B. longum, LBUX23 could be grown on different carbon sources, including glucose (data not shown), sucrose, lactose, and lactulose ( Figure 2 ), and likely, this ability to metabolize carbohydrates is via bifid-shunt, as has been reported in B. longum NCC2705 [ 18 , 43 ]. Additionally, B. longum LBUX23 was grown in fructooligosaccharides (FOS), which are non-digestible carbohydrates, that can be found in various plant-based sources such as onions, asparagus, artichokes, garlic, wheat, bananas, tomatoes, and honey [ 44 ].…”

β-l-Arabinofurano-cyclitol Aziridines Are Covalent Broad-Spectrum Inhibitors and Activity-Based Probes for Retaining β-l-Arabinofuranosidases

“…The intestinal tract represents the predominant colonization site for B. thetaiotaomicron and Bifidobacterium , which are reported to optimally grow in vitro at pH 6.5–7.0. 28 , 29 Recently, in growing B. longum cultures, a two-unit pH reduction from the typical growth conditions (pH 6.5–7.0) for Bifidobacterium was reported upon supplementation of fructooligosaccharide (FOS) in various sugar systems, as a function of FOS concentration and bacterial strain. 29 This growth habit of B. longum suggests that the optimal pH for HypBA1 enzymatic activity may be achieved by the addition of FOS or equivalent nutrients in bacterial growth medium.…”

“… 28 , 29 Recently, in growing B. longum cultures, a two-unit pH reduction from the typical growth conditions (pH 6.5–7.0) for Bifidobacterium was reported upon supplementation of fructooligosaccharide (FOS) in various sugar systems, as a function of FOS concentration and bacterial strain. 29 This growth habit of B. longum suggests that the optimal pH for HypBA1 enzymatic activity may be achieved by the addition of FOS or equivalent nutrients in bacterial growth medium. Irrespective of this hypothesis, the enhanced rate of inhibition of HypBA1 over Bt GH146 in the study presented here ( Table 1 ) may also be due to the greater degree of protonation (and so activation) of the aziridine, already outside the enzyme active site, under the significantly more acidic conditions at which HypBA1 (pH optimum of 4.5) activity is measured than that of Bt GH146 (pH optimum of 7.5).…”

Efficacy of Inulin Supplementation on the Growth and Survivability of Bifidobacterium longum and Bifidobacterium breve in Model Sugar Systems

“…B. breve growth was partially inhibited when fructooligosaccharide concentration was increased (2%−4%), indicating that the bifidobacterial growth is influenced by the degree of polymerization and concentration of oligosaccharides. 20 Rossi et al 17 showed that only a few bifidobacteria are capable of using inulin due to the absence of an extracellular degradation pathway. β-Fructofuranosidase purified from B. infantis ATCC 15697 catalyzes the degradation of inulin by cleaving (2−1) bonds, releasing fructose moieties in the growth media, providing the bacterium with an additional carbon source.…”

“…Previously, dose-dependent effects were observed in B. longum and B. breve when grown for 24 h at 37 °C in 0.5%–4% fructooligosaccharides (DP between 3 and 8). B. breve growth was partially inhibited when fructooligosaccharide concentration was increased (2%–4%), indicating that the bifidobacterial growth is influenced by the degree of polymerization and concentration of oligosaccharides . Rossi et al showed that only a few bifidobacteria are capable of using inulin due to the absence of an extracellular degradation pathway.…”