Growth and Survival of Diploid and Triploid Hybrids of Masu Salmon Oncorhynchus masou.

Oshiro, Takashi; Deng, Ya-Guang; Higaki, Syunji; Takashima, Fumio

doi:10.2331/suisan.57.1851

Cited by 8 publications

(2 citation statements)

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“…For instance, the highest values reported for hatching success of hybrid maternal triploids range from 58% (brook v. brown trout, Galbreath & Thorgaard, 1994) to 83% [rainbow v. coho salmon Oncorhynchus kisutch (Walbaum), Quillet et al , 1988]. Lower values, however, were also reported, which ranged between 1% [masu salmon Oncorhynchus masou (Brevoort) v. rainbow trout, Oshiro et al , 1991] and 14% [chum salmon Oncorhynchus keta (Walbaum) v. brook trout, Gray et al , 1993]. As in the present study, these authors have also invariably used a narrow window to induce hybrid maternal triploidy.…”

Section: Discussionmentioning

confidence: 99%

Maternal and paternal hybrid triploids of tetras

David

Pandian

2006

Journal of Fish Biology

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Hybrid maternal triploids were generated by crossing grey-body and red or yellow-tailed Buenos Aires tetra Hemigrammus caudovittatus (BT) males with albino or black Gymnocorymbus ternetzi [widow tetra (WT)] females and retaining the second polar body by heat shock (HS) or cold shock (CS). Paternal triploids were also generated using 2Á5% polyethylene glycol (PEG) incubated BT semen to facilitate the entry of two sperm into an egg of WT. Optimum temperature for CS was identified as 6°C and the optimum age for thermal shocking the zygote as 3 min after fertilization. At hatching, survival was 25, 17 and 5% for the HS, CS and paternal triploids, respectively. At maturity, it was further reduced to >2% and the maturity inordinately delayed. With the presence of undifferentiated 'steriles' and almost total absence of females, the expected sex ratio was distorted. Triploid males produced spermatocytes, spermatids but not spermatozoa. Triploidy was confirmed by phenotypic markers, karyotyping, erythrocyte measurement and molecular markers. The fusiform body shape of WT was a dominant phenotypic trait over the typical piscine slender body shape of BT. Polymerase chain reaction products of the genomic DNA of the triploids amplified by OPF6 primer were 300, 450 and 1000 bp length, characteristic of BT, and 500 and 800 bp, typical of WT and thereby confirmed the biparental genomic contribution to the triploids. Analyses of genomic DNA of selected progenies using DMRT-1 marker showed that (1) like the diploid BT males, the hybrid triploid males were also true genetic males, and amplified 237 and 300 bp products but (2) the triploid steriles amplified all the three products of 100, 237 and 300 bp indicating that they were mosaics but the diploid steriles failed to amplify.

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Section: Discussionmentioning

confidence: 99%

Maternal and paternal hybrid triploids of tetras

David

Pandian

2006

Journal of Fish Biology

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“…The highest values reported for hatching success of hybrid triploids range from 58% (brook vs. brown trout, Galbreath & Thorgaard 1994) to 83% (rainbow vs. coho salmon, Quillet, Chevassus & Devausx 1988). Lower values, however, were also reported, which ranged between 1% (masu salmon Oncorhynchus keta vs. rainbow trout, Oshiro, Deng, Higachi & Takashima 1991) and 14% (chum salmon Oncorhynchus keta vs. brook trout, Gray, Evans & Thorgaard 1993). Gheyas, Mollah and Hussain (2001) observed lower fertilization and hatching percentage in cold shock treated triploids (91% and 37%) than those of diploid controls (95% and 46%).…”

Section: Fertilization and Hatching Ratesmentioning

confidence: 97%

Triploidization in rohu × mrigal hybrid and comparison of growth performance of triploid hybrid

Rahi

Shah

2011

Aquac Res

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The present study was aimed at the identification of treatment optima to induce triploidy in ‘Labeo rohita (rohu) × Cirrhinus cirrhosus (mrigal)’ hybrid using heat shock treatment. The eggs were exposed at four different temperature regimes viz., 38, 39, 40 and 41°C for 1–3 min, applied 3–5 min after fertilization. After 4 min of fertilization, heat shock treatments for 1 and 1.5 min durations were found the best inducing triploidy up to 100% and 96% respectively. Survival rates upto yolk sac absorption were found to be 73% and 71% in rohu and mrigal, 68% and 67% in the reciprocal diploid hybrids and 61% and 60% in the reciprocal triploid hybrids (RTH). Triploidy was confirmed by chromosome counting that revealed the diploid chromosome number of rohu and mrigal at 2n = 50 and in their triploid hybrid chromosome number was found to be 3n = 75. Growth rate of the RTH showed a significant difference (P < 0.05) from the single species and the diploid hybrids. Triploids also showed higher survival rate over the diploids.

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Chromosomal Genetics II: Polyploidy

Lutz¹

2001

Practical Genetics for Aquaculture

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Growth and Survival of Diploid and Triploid Hybrids of Masu Salmon Oncorhynchus masou.

Abstract: Diploid and triploid fish produced in crosses between female masu salmon (MS) Oncorhynchus masou and males of six species, namely, MS, rainbow trout (RT) O. mykiss, brown

Cited by 8 publications

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Maternal and paternal hybrid triploids of tetras

Maternal and paternal hybrid triploids of tetras

Triploidization in rohu × mrigal hybrid and comparison of growth performance of triploid hybrid

Chromosomal Genetics II: Polyploidy

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