Growth and survival of rohu, Labeo rohita (Hamilton) spawn fed diets supplemented with fish intestinal microflora

Abstract

A 21-day feeding experiment was conducted to assess the effects of diets supplemented with fish intestinal bacteria producing extracellular enzyme on growth and survival of rohu, Labeo rohita (Hamilton) spawn (average weight 0.3 mg). Intestinal bacteria were isolated from healthy rohu fingerlings s averag � w � ight 3.51 _ ±0.31 _ g). Six isocaloric (4.24 kcal·g-) and 1somtrogenous \approximately 35% crude protein) diets (Dl-D6) were prepared containing 32% fish meal, 34% mustard oil cake, 30% rice bran, 2o/o cod liver oil, and 2% vitamin premix. Experimental diets (D2-D6) were supplemented with the isolated bacterial strain Bacillus circu lans Lr 1

Study on Bacillus Isolated from Intestine of Persian Sturgeon (<i>Acipenser persicus</i>) Comparing with Commercial Probiotics

“…Similar to these results, Ghosh et al in 2004 confirmed that bacillus cerculance isolated from intestine of fish species, Labeo rohita, at the level equal to 2  10 8 CFU feed, Changed the weight of fry from 3.45 to 4.61g [21]. According to these results, Ghosh et al in 2002 showed that bacillus isolated from intestine of labeo rohita fish has more effect on increased weight of larvae of this fish than commercial bacillus [12,13].…”

“…In Microbial management, native species isolated from aquatic digestive tract, have played important roles in aquaculture purposes. Similar research were done by Ghosh et al in 2002 with Bacillus circulans isolated from intestine of fish species, Labeo rohita, in diets supplemented with them [12,13]. Also, bacteria Lactobacillus fructivorans isolated from fish species, Sparus auratus, as well as Lactobacillus plantarum (L. plantarum) isolated from human feces, led to increased growth and survival of this fish, after supplementation with Artemia franciscana nauplii [14].…”

“…After unifying rations, they were dried with 40C temperature for 5 hours (10% moisture) and with regard to fish larval requirements were sieved (0.1mm tiny sieve) and were given to them. In control treatment, fish larvae of Persian sturgeon fed ration with no probiotic bacteria [12,13].…”

“…From above dilutions, a volume equal to 100 micro liters were taken and were transported to pellets containing culture medium of Bacillus cereus agar and triptic soy agar (TCA) and were distributed in it. The above pellets were incubated in incubator for 24 hours in temperature of 30C and forming colonies again were purely cultured in culture (TCA) medium [5,12,13].…”

“…Bacterial rates' increasing was determined by each ration equal to one single logarithmic unit. Similarly, also in Lactobacillus group, the experimental rations were prepared with these bacterial concentrations from their powder products and were mixed with other rations and their homogenation suspension was prepared [12,13,16].…”