This investigation aimed to find out the effect of chemical nitrogen fertilizer levels (15, 30,45 and 60 kg N/fad.), barley seeding rate (40,60 and 80 kg/fad.) and nanofertilizer application on yield and its components of barley. The spiltspilt plot design as a form of the randomized complete block design with three replications was followed.Application of nano-fertilizer appeared to produce high values in No. of both spike lets and grains per spike as well as the heavy grains/spike, 1000-grain weight and, increased grain yield/fad. Seeding rate of 80 kg/fad. appeared to produce the highest value in each of number of spikes/m 2 and grain yield/fad. Meanwhile, the seeding rate of 40 kg/fad. recorded the highest value in each of No. of both spike lets and grains per spike, weight of both grains/spike and 1000-grain . Nitrogen fertilization was very effective on all yield characteristics, so any increase in N levels was followed by a significant increase in each of No of spike lets/spike, grains/spike and spikes/m 2 , also increased weight of both grains/spike and 1000-grain weight and grain yield per fa.Conclusively, these results finally recommend sowing barley with dense seeding rate of 80 kg/fad. and application of 60 kg N/ fad. In addition to application of nano-fertilizer to maximize grain yield /fad. under the experimental site and other likely environmental conditions.