Growth and yield of Pleurotus ostreatus using sugarcane bagasse as an alternative substrate in Malaysia

Abstract

This paper looks at the feasibility of using sugarcane bagasse (SGB) for the cultivation of Pleurotus ostreatus (oyster mushrooms) as an alternative for rubber tree sawdust (RS). Three different compositions of oil palm trunk and rubber tree sawdust were studied with each composition having three replicates. A substrate containing only rubber tree sawdust acted as the control variable. The substrates were supplemented with 5% rice bran and 1.5% calcium carbonate. There are four steps involved in the cultivation of P. ostreatus which were bagging, sterilization, spawning and harvesting the substrates. The mycelia growth, spawn run, pin head formation, yield and biological efficiency were observed. The 100% composition of RS gave the best yield in terms of the mean height of stipe, which was 7.75cm. The mean fresh weight of sawdust was the highest with 50.4g with the number of fruiting bodies around 6. The least yield was recorded for 50% composition of substrates with a mean height of stipe 9.12cm. Among the substrates used, the RS showed the highest biological efficiency with 56.3% followed by 45.1% and 44.8% RS having mixed compositions of 25% and 50% SGB substrates, respectively. Hence, it is proven that in terms of quantity and quality, the unmixed substrates produced better edible mushrooms. However, the results obtained by SGB also showed a great potential as an alternative substrate for the cultivation of P. ostreatus when mixed with RS.

Analysis of Biochemical and Genetic Variability of Pleurotus ostreatus Based on the β-Glucans and CDDP Markers

“…The fertility parameter of individual strains may be significant, as there is evidence that the biological efficiency of individual strains may vary [ 62 , 63 , 64 ]. Pleurotus ostreatus species is quite unique because they are able to grow on different agrowaste materials [ 65 ], such as rice straw [ 66 ], oil palm fronds [ 67 ], empty fruit bunch and palm-pressed fiber [ 68 ], sugarcane bagasse [ 69 ], corn cob [ 70 ] wheat straw, coffee waste, and leaf litter of forest trees [ 71 ]. If the strain is characterized by a high content of β-glucans but is low in crop, it is not an important source of biologically valuable substances for the producer.…”

Utilization of Agricultural Waste for the Cultivation of Pleurotus Mushrooms in Thailand

“…5,21,22 Various studies suggested that sugarcane bagasse is a good source for the cultivation of P. ostreastus. 23,24 Their studies showed that biomass from sugarcane either used alone, or in combination with other substrates, such as rubber tree sawdust and wheat straw, can increase the yield of oyster mushrooms. In this study, P. ostreatus PO3 also grew well on sugarcane bagasse; however, P. pulmonarius PP6 hardly grew on the same substrate.…”

Substrate Optimization for Shiitake (Lentinula edodes (Berk.) Pegler) Mushroom Production in Ethiopia

“…Typically, an 80% hardwood sawdust and 20% additive mixture is used as the standard substrate formula for shiitake cultivation [ 23 ]. However, this study used a starch-based substrate (sugarcane bagasse) as the main substrate as used by [ 24 , 25 ] and supplemented it with nutrient additives such as cow dung, horse manure, chicken manure, or sugarcane filter cake, which we assumed would enhance mushroom yields due to their high nutrient content [ 26 ]. Mushrooms can grow and provide higher yields when using a substrate of optimal C:N [ 27 ].…”