Growth arrest and rapid capture of select pathogens following magnetic nanoparticle treatment

Niemirowicz, Katarzyna; Święcicka, Izabela; Wilczewska, Agnieszka Z.; Markiewicz, Karolina H.; Surel, Urszula; Kułakowska, Alina; Namiot, Z; Szynaka, B; Bucki, Robert; Car, Halina

doi:10.1016/j.colsurfb.2015.04.016

Cited by 32 publications

(26 citation statements)

References 42 publications

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“…25,30 Stöber methods were used to obtain core-shell magnetic nanostructures with terminal propylamine groups. 26,31 Immobilization of CHX onto the surface of nanoparticles was achieved by interaction between the primary amine group of nanoparticles and the chloride group from CHX. After anchoring, the precipitate was magnetically collected, washed with ethanol and phosphate-buffered saline (PBS) and dried at 50°C.…”

Section: Materials and Methods Synthesis Of Magnetic Nanoparticles Fumentioning

confidence: 99%

“…These nanoparticles are able to interact with planktonic bacteria as well as those embedded in a biofilm matrix. 26,27 The general mechanism of nanoparticle toxicity against pathogens involves binding to the cell wall and subsequent disruption of the membrane through either direct interaction and/or oxidation of macromolecules. 28 Interestingly, recent reports indicate that metal-oxide nanoparticles are nontoxic to mammalian cells due to their ability to phagocytose and degrade nanoparticles through lysosomal fusion.…”

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confidence: 99%

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Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Tokajuk

Niemirowicz

Deptuła

et al. 2017

IJN

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Nanotechnology offers new tools for developing therapies to prevent and treat oral infections, particularly biofilm-dependent disorders, such as dental plaques and endodontic and periodontal diseases. Chlorhexidine (CHX) is a well-characterized antiseptic agent used in dentistry with broad spectrum activity. However, its application is limited due to inactivation in body fluid and cytotoxicity toward human cells, particularly at high concentrations. To overcome these limitations, we synthesized nanosystems composed of aminosilane-coated magnetic nanoparticles functionalized with chlorhexidine (MNP@CHX). In the presence of human saliva, MNPs@CHX displayed significantly greater bactericidal and fungicidal activity against planktonic and biofilm-forming microorganisms than free CHX. In addition, CHX attached to MNPs has an increased ability to restrict the growth of mixed-species biofilms compared to free CHX. The observed depolarization of mitochondria in fungal cells treated with MNP@CHX suggests that induction of oxidative stress and oxidation of fungal structures may be a part of the mechanism responsible for pathogen killing. Nanoparticles functionalized by CHX did not affect host cell proliferation or their ability to release the proinflammatory cytokine, IL-8. The use of MNPs as a carrier of CHX has great potential for the development of antiseptic nanosystems.

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Section: Materials and Methods Synthesis Of Magnetic Nanoparticles Fumentioning

confidence: 99%

mentioning

confidence: 99%

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Tokajuk

Niemirowicz

Deptuła

et al. 2017

IJN

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“…52 The proposed mechanism of antibacterial action of core-shell MNP involves their contribution to interaction with components of pathogen membranes, such as protein F, and restriction of their growth. 17,18 We also observed that nanoparticles interact with bacterial and fungal membranes and are internalized into cells. 18 The proposed mechanism of synergistic action of core-shell nanoparticles with membrane-active agents is summarized in Figure 4.…”

Section: Discussionmentioning

confidence: 81%

“…17,18 We also observed that nanoparticles interact with bacterial and fungal membranes and are internalized into cells. 18 The proposed mechanism of synergistic action of core-shell nanoparticles with membrane-active agents is summarized in Figure 4.…”

Section: Discussionmentioning

confidence: 81%

“…[13][14][15][16] Our recent studies indicate that core-shell MNPs might act as antimicrobial agents via destruction of bacterial cell membrane and interaction with surface proteins. 17,18 Furthermore, it has been reported that nanoparticles penetrate biofilms, destroy spore-forming bacteria, and are active against MDR pathogens. 17,19,20 The enhancement of the efficiency of antimicrobial agents due to their covalent or electrostatic immobilization on the surface of the nanoparticles and cotreatment using nanomaterials containing combinations is a preferable approach in the treatment of bacterial and fungal infections.…”

Section: Introductionmentioning

confidence: 99%

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Core–shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins

Niemirowicz¹,

Piktel²,

Wilczewska³

et al. 2016

IJN

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Core–shell magnetic nanoparticles (MNPs) are promising candidates in the development of new treatment methods against infections, including those caused by antibiotic-resistant pathogens. In this study, the bactericidal activity of human antibacterial peptide cathelicidin LL-37, synthetic ceragenins CSA-13 and CSA-131, and classical antibiotics vancomycin and colistin, against methicillin-resistant Staphylococcus aureus Xen 30 and Pseudomonas aeruginosa Xen 5, was assessed alone and in combination with core–shell MNPs. Fractional inhibitory concentration index and fractional bactericidal concentration index were determined by microdilution methods. The potential of combined therapy using nanomaterials and selected antibiotics was confirmed using chemiluminescence measurements. Additionally, the ability of tested agents to prevent bacterial biofilm formation was evaluated using crystal violet staining. In most conditions, synergistic or additive effects were observed when combinations of core–shell MNPs with ceragenins or classical antibiotics were used. Our study revealed that a mixture of membrane-active agents such as LL-37 peptide or ceragenin CSA-13 with MNPs potentialized their antibacterial properties and might be considered as a method of delaying and overcoming bacterial drug resistance.

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Antibacterial Capabilities of Metallic Nanoparticles and Influencing Factors

Girma,

Mebratie,

Mekuye

et al. 2024

Nano Select

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The increase of antibiotic resistance in bacteria has become a major concern for successful diagnosis and treatment of infectious diseases. Over the past few decades, significant progress has been achieved on the development of nanotechnology‐based medicines for combating multidrug resistance in microorganisms. Among these, metallic nanoparticles (MNPs) hold great promise in addressing this challenge due to their broad‐spectrum and robust antimicrobial properties. This review illustrates the antibacterial activities of MNPs and further elucidates how different factors including synthesis method, size, shape, surface charge, pH, dose, type of capping or stabilizing agents of MNPs, and Gram‐type of the bacteria, impact their antibacterial activities, which are expected to promote the future development of more potent MNP‐based antibacterial agents.

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Growth arrest and rapid capture of select pathogens following magnetic nanoparticle treatment

Cited by 32 publications

References 42 publications

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Core–shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins

Antibacterial Capabilities of Metallic Nanoparticles and Influencing Factors

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Growth arrest and rapid capture of select pathogens following magnetic nanoparticle treatment

Cited by 32 publications

References 42 publications

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Core&ndash;shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins

Antibacterial Capabilities of Metallic Nanoparticles and Influencing Factors

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Core–shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins