Growth assay of individual pollen tubes arrayed by microchannel device

Horade, Mitsuhiro; Yanagisawa, Naoki; Mizuta, Yoko; Higashiyama, Tetsuya; Arata, Hideyuki

doi:10.1016/j.mee.2014.01.017

Cited by 13 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The viability of the tubes is not affected as in vitro growth rates with a high variability of 100–500 nm/sec have been reported in the literature [39,44]. Torenia fournieri pollen tubes grown in microchannels have been reported to show a 2.5 times enhanced growth rate compared to normal liquid medium assay (n = 16), and it was speculated that the microchannels mimic an in vivo growth environment for the pollen tubes [15]. In the TipChip [13], it was observed that changing the microchannel-height to tube-diameter ratio from 4.7 to 9.4 increased the growth rate by up to 50% (n = 3) for Camellia japonica , but no control data on conventional in vitro growth rates was presented.…”

Section: Resultsmentioning

confidence: 98%

“…Higashiyama and coworkers have used Torenia fournieri to study pollen tube guidance and pollen tube-female tissue interaction and A . thaliana ovules for long-term live imaging [15,16] using specialized LoCs. All but one [15] of the above mentioned systems for pollen tubes studies lack the tight vertical confinement of the tip-growing cell in a single focal plane, which is crucial for long-term optical imaging and monitoring.…”

Section: Introductionmentioning

confidence: 99%

“…thaliana ovules for long-term live imaging [15,16] using specialized LoCs. All but one [15] of the above mentioned systems for pollen tubes studies lack the tight vertical confinement of the tip-growing cell in a single focal plane, which is crucial for long-term optical imaging and monitoring. The devices have a uniform height to accommodate the large size of the grain in comparison to the pollen tube, while Horade and colleagues cleverly avoided the need for a multi-height device by introducing a hand-pollinated style directly into the LoC [15].…”

Section: Introductionmentioning

confidence: 99%

“…All but one [15] of the above mentioned systems for pollen tubes studies lack the tight vertical confinement of the tip-growing cell in a single focal plane, which is crucial for long-term optical imaging and monitoring. The devices have a uniform height to accommodate the large size of the grain in comparison to the pollen tube, while Horade and colleagues cleverly avoided the need for a multi-height device by introducing a hand-pollinated style directly into the LoC [15]. The throughput of most existing LoC-based assays is restricted, however, as only a limited number of pollen tubes could be incorporated, guided, and observed on the chip at a time.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Massively Parallelized Pollen Tube Guidance and Mechanical Measurements on a Lab-on-a-Chip Platform

et al. 2016

View full text Add to dashboard Cite

Pollen tubes are used as a model in the study of plant morphogenesis, cellular differentiation, cell wall biochemistry, biomechanics, and intra- and intercellular signaling. For a “systems-understanding” of the bio-chemo-mechanics of tip-polarized growth in pollen tubes, the need for a versatile, experimental assay platform for quantitative data collection and analysis is critical. We introduce a Lab-on-a-Chip (LoC) concept for high-throughput pollen germination and pollen tube guidance for parallelized optical and mechanical measurements. The LoC localizes a large number of growing pollen tubes on a single plane of focus with unidirectional tip-growth, enabling high-resolution quantitative microscopy. This species-independent LoC platform can be integrated with micro-/nano-indentation systems, such as the cellular force microscope (CFM) or the atomic force microscope (AFM), allowing for rapid measurements of cell wall stiffness of growing tubes. As a demonstrative example, we show the growth and directional guidance of hundreds of lily (Lilium longiflorum) and Arabidopsis (Arabidopsis thaliana) pollen tubes on a single LoC microscopy slide. Combining the LoC with the CFM, we characterized the cell wall stiffness of lily pollen tubes. Using the stiffness statistics and finite-element-method (FEM)-based approaches, we computed an effective range of the linear elastic moduli of the cell wall spanning the variability space of physiological parameters including internal turgor, cell wall thickness, and tube diameter. We propose the LoC device as a versatile and high-throughput phenomics platform for plant reproductive and development biology using the pollen tube as a model.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Massively Parallelized Pollen Tube Guidance and Mechanical Measurements on a Lab-on-a-Chip Platform

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Such technology can be used to develop a microenvironment mimicking that within the pistil during in vivo PT growth. Given these advantages, PT growth rate measurement has become possible under conditions that closely simulate the in planta environment (10). A microsystem-based assay has been successfully adopted to develop a microfluidic network to examine the cellular penetrative forces (11) and the device was further modified for experimentation and phenotyping of PTs (12).…”

mentioning

confidence: 99%

Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway

Sato

Sugimoto

Higashiyama

et al. 2015

Journal of Bioscience and Bioengineering

Self Cite

View full text Add to dashboard Cite

show abstract

An Introduction to Male Germline Development

Vogler

Grossniklaus

Hedhly

2017

Methods in Molecular Biology

View full text Add to dashboard Cite

In this introductory chapter, we describe male germline development in plants taking Arabidopsis thaliana as a reference species. We first describe the transition from sporophytic to germline development, then microsporogenesis including meiosis, followed by male gametophyte development prior to pollination, and finally the progamic phase culminating in double fertilization, which leads to the formation of the embryo and the endosperm. For detailed information on some of these processes or on the molecular underpinning of certain fate transitions, we refer the reader to recent reviews. An important but often neglected aspect of male gametophyte development is the formation of the unique pollen cell wall. In contrast to that of other plant cells, the pollen cell wall is composed of two principal layers, the intine and exine. While the intine, the inner pecto-cellulosic cell wall layer, is biochemically and structurally similar to a "classical" plant cell wall, the exine is a unique composite with sporopollenin as its main component. Biosynthesis of the cell wall is remarkably similar between the spores of mosses and ferns, and pollen of seed plants, although slight differences exist, even between closely related species (reviewed in Wallace et al., AoB Plants 2011:plr027, 2011). In the latter sections of this chapter, we will present a brief overview of cell wall development in Arabidopsis pollen, where this aspect has been intensively studied.

show abstract

Growth assay of individual pollen tubes arrayed by microchannel device

Cited by 13 publications

References 20 publications

Massively Parallelized Pollen Tube Guidance and Mechanical Measurements on a Lab-on-a-Chip Platform

Massively Parallelized Pollen Tube Guidance and Mechanical Measurements on a Lab-on-a-Chip Platform

Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway

An Introduction to Male Germline Development

Contact Info

Product

Resources

About