Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells

Murata, Takayuki; Takashima, Yasuhiro; Xuan, Xuen; Otsuka, Hidenori

doi:10.1016/s0168-1702(99)00023-4

Cited by 11 publications

(21 citation statements)

References 42 publications

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“…20 Myxoma virus, a rabbit poxvirus with a similar restricted tropism, functions as an oncolytic virus in human tumor cells bearing deficiencies in type I IFN and tumor necrosis factor-a (TNFa) pathways. 23,24 Before investigating the oncolytic capacity of BHV-1, we first confirmed the restriction of normal human cells against BHV-1 replication and CPE induction.…”

Section: Bhv-1 Replication Is Restricted In Normal Human Cellsmentioning

confidence: 99%

“…19 One of the interesting features of BHV-1 is its strict host range compared with other herpesviruses, including HSV-1. Of particular interest is the inability of BHV-1 to productively infect human cells, 20 supported by the lack of reports of either productive BHV-1 infection or even 21 In this study, we determined the ability of BHV-1 to replicate, cause cytopathic effects and affect cellular viability in a panel of normal, immortalized and transformed human cells from a variety of histological origins. We found that BHV-1 replicates and induces cytopathic effects in a wide subset of immortalized and transformed cells, thus decreasing cellular viability, but is significantly restricted in normal cells.…”

Section: Introductionmentioning

confidence: 97%

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Bovine herpesvirus type 1 as a novel oncolytic virus

2009

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Oncolytic virotherapy is a promising avenue of cancer gene therapy. Current vectors include human viruses that have been engineered to replicate in tumor cells or nonhuman viruses that are naturally oncotropic and preferentially replicate in tumor cells harboring defects in innate immune pathways such as the type 1 interferon (IFN) pathway. Bovine herpesvirus type 1 (BHV-1) is a species-specific herpesvirus closely related to the human herpes simplex virus type 1 (HSV-1). Although BHV-1 does not efficiently replicate in and affect cellular viability of normal human cells, it is capable of infecting and killing various immortalized and transformed human cell types. Surprisingly, BHV-1 infection of human cells fails to elicit IFN production at the mRNA or protein level and the ability of BHV-1 to kill immortalized and transformed human cells does not correlate with defects in IFN pathways. Furthermore, although some cross-reactivity between BHV-1 and HSV-1 exists, the majority of human antibody or serum samples tested failed to neutralize BHV-1 despite possessing HSV-1 neutralizing capacity. Thus, BHV-1 is a novel candidate oncolytic virus with a distinct mechanism of tumor targeting.

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Section: Bhv-1 Replication Is Restricted In Normal Human Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Bovine herpesvirus type 1 as a novel oncolytic virus

2009

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“…1E, G and I for MDBK, CER and CRIB cells, respectively. In fact, there are many advantages in using serum-free culture systems; first it has been suggested that cells cultured without serum/protein seems to be more favourable for viral infection than cultured by using the serum-supplemented medium; second bovine serum may contains viral inhibitors, such proteases, that decreases the virus stability; and third bovine serum may contains exogenous pathogens and also prions [7,13,14,15] .…”

Section: Resultsmentioning

confidence: 99%

Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Cardoso¹,

Ferrari²,

Luvizotto³

et al. 2007

American J. of Biochemistry and Biotechnology

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Abstract:The detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5µg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in a statistic continuous culture system. The results obtained by CRIB and MDBK cells adaptation showed steady growth after 10 th passages in OPTI-PRO medium, 20 th passages in VP-SFM medium and 30 th passages in RPMI 1640 medium, respectively. The OPTI-PRO and RPMI 1640 media were not able to support CER growth, even supplemented by IGF-1 and present apoptotic cells at 72h post-infection. The CER cells seeded after adaptation by 10 th passages in VP-SFM medium added by 5µg/20ml of IGF-1 growth factor revealed the best virus titres compared to MDBK and CRIB cell lines. It was concluded that CER, MDBK and CRIB cells infected by BoHV-5 serotype could be used for laboratory diagnosis propagated on much safer culture system of high biotechnology advances.

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“…Murata et al ( Murata T et al 1999 ) studied the effect of the MOI in the production of BoHV-1 in MDBK and found that the titer of the virus did not differ in view of the MOI used, but in HmLu cells, there is an important effect of the MOI. In 293 cells infected by adenovirus, the MOI had a progressive effect on viral production both in culture and in stationary MCs ( Kapil S, Basaraba RJ.…”

Section: Discussionmentioning

confidence: 99%

Parameters for MDBK cell growth on microcarriers and BoHV-1 virus production

Freitas¹,

Mozzer²,

Freitas³

et al. 2017

VR&R

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Bovine herpes virus 1 (BoHV-1) is an important veterinary agent , which causes infectious bovine rhinotracheitis. This disease affects the respiratory tract or genitals, causing weight loss, reduced milk production and abortion. Several vaccines against BoHV-1 have been developed. In this paper, we study the parameters for MDBK growth on microcarriers (Cytodex 1) and for BoHV-1 virus production. The cell culture attached to microcarriers is an efficient method to enlarge the surface of cell growth and for large-scale cell production. Our studies reveal that MDBK adhered to MCs in 30 minutes and that initial agitation of culture did not influence on the efficiency of adhesion or cell growth. In our experiments, we detected no relevant influence of agitation on initial cell adhesion of MDBK to MCs. The maximum cell yield was similar to all initial conditions of agitation studied. The maximum yield obtained in culture started with 15, 20 and 30 cells / MC, was respectively.8.7 x105, 9.3 x105 and 9.8 x105 cells / ml . The cellular distribution on the MCs at the beginning of the culture was more heterogeneous in higher initial densities. After three medium exchanges during MDBK cell culture, the increase in the final yield was 100% higher than that from culture performed without medium change (0.93 x 106 cells / mL). Replacing 50% of the culture medium with fresh medium after 24 hours of growth, the concentration of glucose (5 mM) and glutamine (1.8 mM) were almost completely restored. In these studies, BoHV-1 infections of MDBK were performed after 48, 72 and 86 hours with daily exchanges of 50% of the medium. The increase in viral titer was proportional to the number of viable cells present at the time of infection. The best result of BoHV-1 production was achieved when the infection was performed from 86 hours of cell culture, reaching about 3.7 x108 (TCID50/ml) after 24-48 hours of infection, being on average four times higher when compared to the culture in which the infection was performed with 48 hours of culture and approximately 2 times greater than the crop whose infection occurred after 72 hours of culture. The best yields are obtained when viral infections were performed on cultures with higher cell densities. The best result for the production of BoHV-1 occurred with 10 MOI, 48 hours after infection, which yield was 24 and 41% superior to the MOI 0.1 and 1, respectively.

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Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells

Cited by 11 publications

References 42 publications

Bovine herpesvirus type 1 as a novel oncolytic virus

Bovine herpesvirus type 1 as a novel oncolytic virus

Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Parameters for MDBK cell growth on microcarriers and BoHV-1 virus production

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