Growth, branching and enzyme production by filamentous fungi in submerged culture

Trinci, Anthony P. J.; Bocking, Sharon P.; Swift, Richard J.; Withers, Julie M.; Robson, G. D.; Wiebe, Marilyn G.

doi:10.1017/cbo9780511549694.006

Cited by 5 publications

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“…Data are also available to suggest their production is shear-sensitive, independently of O 2 levels (Jayus et al, 2005b). No evidence was obtained to support the popular view that exocellular levels of these enzymes in Acremonium IMI 383068 might be related to the number of hyphal tips and branching frequencies of the producing fungus (Jayus et al, 2002(Jayus et al, , 2005a, the possible sites for their secretion Trinci et al, 1999). Similar data for other fungal b-glucanases are sparse and limited to screening influences of different carbon and nitrogen sources on enzyme yields, often in shake flasks where critical culture parameters are not controllable, and culture variables may interact (Tweddell et al, 1994;Pitson et al, 1997b;Vasquez-Garciduenas et al, 1998).…”

Section: Fermentation Studies and Enzyme Yields?mentioning

confidence: 94%

Biochemistry and molecular biology of exocellular fungal β-(1,3)- and β-(1,6)-glucanases

et al. 2007

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Many fungi produce exocellular beta-glucan-degrading enzymes, the beta-glucanases including the noncellulolytic beta-(1,3)- and beta-(1,6)-glucanases, degrading beta-(1,3)- and beta-(1,6)-glucans. An ability to purify several exocellular beta-glucanases attacking the same linkage type from a single fungus is common, although unlike the beta-1,3-glucanases, production of multiple beta-1,6-glucanases is quite rare in fungi. Reasons for this multiplicity remain unclear and the multiple forms may not be genetically different but arise by posttranslational glycosylation or proteolytic degradation of the single enzyme. How their synthesis is regulated, and whether each form is regulated differentially also needs clarifying. Their industrial potential will only be realized when the genes encoding them are cloned and expressed in large quantities. This review considers what is known in molecular terms about their multiplicity of occurrence, regulation of synthesis and phylogenetic diversity. It discusses how this information assists in understanding their functions in the fungi producing them. It deals largely with exocellular beta-glucanases which here refers to those recoverable after the cells are removed, since those associated with fungal cell walls have been reviewed recently by Adams (2004). It also updates the earlier review by Pitson et al. (1993).

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