Growth capability of epidemic influenza viruses in Japan since the 2009 H1N1 pandemic

Tsuneki-Tokunaga, Akeno; Kanai, Koomi; Itagaki, Asao; Tsuchie, Hideaki; Okada, Takayoshi; Kasagi, Masaaki; Tanaka, Kiyoshi; Aoki, Miho; Hinay, Alfredo A.; Kageyama, Seiji

doi:10.1007/s00705-021-04976-5

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…Total RNA was extracted using a QIAamp Viral RNA Mini Kit (QIAGEN, Tokyo, Japan). Eight RNA segments of in uenza A virus were simultaneously reverse transcribed and ampli ed using a SuperScript™III One Step RT-Polymerase Chain Reaction (PCR) System (Life Technologies, Tokyo, Japan) with the MBTuni-12/MBTuni-13 primer pair [5,20]. The rst-round PCR products were subjected to a second PCR to amplify NS1 using A-NS-M13 primers (sense: 5-TGTAAAACGACGGCCAGTAGCAAAAGCAGGGTGACAAAGACA-3; antisense: 5-CAGGAAACAGCTATGACCAGTAGAAACAAGGGTGTTTTTTAT-3).…”

Section: Ns1 Sequencingmentioning

confidence: 99%

“…The clinical severity of in uenza virus infection can range from asymptomatic to severe symptomatic illness, and it has been shown that a higher viral load is associated with a more severe case of the disease and a high probability of virus transmission and spread [3,4]. Moreover, one of the probable factors affecting viral load is the intrinsic viral growth capability of the virus [5].…”

Section: Introductionmentioning

confidence: 99%

“…Our previous studies demonstrated emerging pandemic viruses consisted of only highly replicative strains, but more-slowly replicating strains were observed soon in the following seasons [5,6]. We hypothesized that the difference in growth capability could be due to the immune response of the human host, and understanding how viral load and host immune responses are related is critical for identifying disease-speci c markers that may help predict disease prognosis.…”

Section: Introductionmentioning

confidence: 99%

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Suppression of Interferon Stimulated Genes Enhance Viral Replication of Seasonal Influenza A Virus through the JAK/STAT pathway

Kakee

Kageyama

et al. 2022

Preprint

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Background In a previous study, we described the diverse growth capabilities of circulating seasonal influenza A virus (IAV) with low to high viral copies in vitro. In this study, we analyzed the cause of differences in growth capability by evaluating Interferon-β and antiviral interferon-stimulated genes (ISGs) expression. Methods A549 cells (3.0x105 cells) were inoculated with circulating seasonal IAV strains for 6 and 24 h. In cells inoculated for 6 h, IAV production was assessed by IAV-RNA copies in culture supernatant and cell pellets for gene expression evaluation. At 24 hours post-infection, cells were collected for IFN-β and ISG-15 protein expression. JAK/STAT pathway inhibitor, pyridone 6 (P6) was used to evaluate the influence of ISGs on the growth capability of seasonal IAV. Results A549 cells inoculated with seasonal IAV strains with high growth capability expressed less IFN-β and ISGs, whereas low growth capability strains expressed more IFN-β and ISGs. The use of a P6, a JAK/STAT inhibitor, suppressed the expression of ISGs and enhanced the viral copies of seasonal IAV strains with low growth capability. This result suggests that growth capability is regulated by ISG-mediated IFN-β production. Conclusion In this study, we provide a new insight into the mechanism of circulating seasonal IAV replication. Our study showed that the difference in the growth capability of circulating seasonal IAV strains is due to the regulation of antiviral ISGs. Inhibition of the JAK/STAT pathway permits seasonal IAV strains with low growth capability to enhance their viral copies by suppressing antiviral ISGs. Our results may contribute in developing new effective therapeutic strategies and reduce the risk of developing severe influenza disease.

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Section: Ns1 Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Suppression of Interferon Stimulated Genes Enhance Viral Replication of Seasonal Influenza A Virus through the JAK/STAT pathway

Kakee

Kageyama

et al. 2022

Preprint

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Mixed polysaccharides derived from shiitake mushroom, Poriacocos, Ginger, and Tangerine peel prevent the H1N1 virus infections in mice

Yang

Zhu

et al. 2021

Bioscience, Biotechnology, and Biochemistry

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The pandemic influenza A (H1N1) virus spread globally and posed one of the most serious global public health challenges. The traditional Chinese medicine is served as a complementary treatment strategy with vaccine immunization. Here, we demonstrated the mixed polysaccharides (MPs) derived from shiitake mushroom, poriacocos, ginger and tyangerine peel prevent the H1N1 virus infections in mice. MPs pretreatment attenuated H1N1 virus-induced weight loss, clinical symptoms and death. The lymphocytes detection results showed the CD3+, CD19+ and CD25+ cell proportions were up-regulated in thymus under MPs pretreatment. Besides, MPs pretreatment reduced the inflammatory cell infiltration and increased the cell proportions of CD19+, CD25+ and CD278+ in lung. However, MPs treatment have no effective therapeutic effect after H1N1 virus challenge. The current study suggested that pretreatment with MPs could attenuate H1N1 virus-induced lung injury and up-regulate humoral and cellular immune responses in non- immunized mice.

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Pro-Inflammatory Cytokines and Interferon-Stimulated Gene Responses Induced by Seasonal Influenza A Virus with Varying Growth Capabilities in Human Lung Epithelial Cell Lines

Hinay

Kakee²,

Kageyama³

et al. 2022

Vaccines

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In a previous study, we described the diverse growth capabilities of circulating seasonal influenza A viruses (IAVs) with low to high viral copy numbers in vitro. In this study, we analyzed the cause of differences in growth capability by evaluating pro-inflammatory cytokines (TNF-α, IL-6, IFN-β) and antiviral interferon-stimulated genes (ISG-15, IFIM1, and TRIM22). A549 cells (3.0 × 105 cells) were inoculated with circulating seasonal IAV strains and incubated for 6 and 24 h. In cells inoculated for 6 h, IAV production was assessed using IAV-RNA copies in the culture supernatant and cell pellets to evaluate gene expression. At 24 h post-infection, cells were collected for IFN-β and ISG-15 protein expression. A549 cells inoculated with seasonal IAV strains with a high growth capability expressed lower levels of IFN-β and ISGs than strains with low growth capabilities. Moreover, suppression of the JAK/STAT pathway enhanced the viral copies of seasonal IAV strains with a low growth capability. Our results suggest that the expression of ISG-15, IFIM1, and TRIM22 in seasonal IAV-inoculated A549 cells could influence the regulation of viral replication, indicating the existence of strains with high and low growth capability. Our results may contribute to the development of new and effective therapeutic strategies to reduce the risk of severe influenza infections.

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Growth capability of epidemic influenza viruses in Japan since the 2009 H1N1 pandemic

Cited by 3 publications

References 23 publications

Suppression of Interferon Stimulated Genes Enhance Viral Replication of Seasonal Influenza A Virus through the JAK/STAT pathway

Suppression of Interferon Stimulated Genes Enhance Viral Replication of Seasonal Influenza A Virus through the JAK/STAT pathway

Mixed polysaccharides derived from shiitake mushroom, Poriacocos, Ginger, and Tangerine peel prevent the H1N1 virus infections in mice

Pro-Inflammatory Cytokines and Interferon-Stimulated Gene Responses Induced by Seasonal Influenza A Virus with Varying Growth Capabilities in Human Lung Epithelial Cell Lines

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