A new gram-positive bacterium was isolated from activated sludge acclimated with sugar-containing synthetic wastewater. This organism, designated strain Y-104T (T = type strain), was a coccus-shaped, aerobic chemoorganotroph that had a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor. This strain accumulates large amounts of polysaccharide in its cells. Strain Y-104T has the following chemotaxonomic characteristics: it contains menaquinone MK-8(H4), its DNA G+C content is 67.5 mol%, and it contains meso-diaminopimelic acid. No previously described high-G+ C-content gram-positive coccus contains both MK-8(H4) as a major quinone and meso-diaminopimelic acid in its cell wall. A phylogenetic analysis based on 16s rRNA sequences showed that strain Y-104T represents a line of descent distinct from those of previously described species of high-G+C-content gram-positive bacteria and that members of the genus Frankiu are the nearest neighbors. Therefore, we concluded that our isolate should be assigned to a new genus and species, for which we propose the name Microsphueru multipurtita. The type strain is strain Y-104.Activated sludge is a rich source of aerobic chemoorganotrophic bacteria which belong to various taxonomic and phylogenetic groups, and some of these organisms may not have been described yet. The community structure of activated sludge systems varies remarkably in response to the chemical nature of the wastewater and the operational conditions, and bacteria capable of rapid uptake and accumulation of substrates from the wastewater may predominate under substrate-limiting conditions, particularly when substrate addition is intermittent (for example, in batch treatment processes). The fact that activated sludges contain large amounts of reserve materials, such as glycogen, polyhydroxyalkanoates, and polyphosphate, under certain conditions suggests that there are bacterial species that have the ability to accumulate these reserve materials. Our recent study demonstrated that a new polyphosphateaccumulating gram-positive bacterium (22), for which we proposed the name Microlunatus phosphovonis (21), is present in activated sludge showing enhanced phosphate removal.The bacterial composition and capacity for polysaccharide accumulation of activated sludge treating carbohydrate-containing wastewater have been major subjects of study (27)(28)(29)(30)34). Early reports in the literature showed that large coccusshaped gram-positive bacteria constituted significant proportions of the bacterial flora of activated sludge loaded with carbohydrate-rich wastewater (28, 30), although no detailed taxonomic study of these gram-positive cocci was performed thereafter. Activated sludge cultured under batch operational conditions with intermittent addition of substrate containing glucose showed rapid substrate uptake and accumulated substrate as polysaccharide which was later consumed slowly for the synthesis of DNA, RNA, and protein (20). Accordingly, to elucidate the mechanism of selection o...