GROWTH CHARACTERISTICS OF
            <i>MYCOBACTERIUM AVIUM</i>
            AND GROUP III NONPHOTOCHROMOGENIC MYCOBACTERIA IN HELA CELLS

Brosbe, Edwin A.; Sugihara, Paul T.; Smith, C. R.

doi:10.1128/jb.84.6.1282-1286.1962

Cited by 5 publications

(1 citation statement)

References 7 publications

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“…The results obtained for growth of M. avium in the culture medium described in this report may not represent the activity of the pathogen in vivo. M. avium growing intracellularly was filamentous both in cell culture (6) and also in infected animals (23,30). However, antibiotics were used in the cell culture experiments (6), and most infections in laboratory animals were produced with overwhelming numbers of mycobacteria (23,31).…”

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confidence: 98%

Effect of Palmitic Acid Utilization on Cell Division in Mycobacterium avium

McCarthy

1974

Infect Immun

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Mycobacterium avium, a facultative pathogen for humans, undergoes a life cycle in which selected small cells elongate and then fragment to form coccobacilli. M. avium cells of uniform size were selected by membrane filtration and tested for growth and division in the presence or absence of palmitic acid. Growth was measured by increased cellular protein, and cell division was determined by increased colony-forming units on agar or, electronically, by increased numbers of particles. Both growth and division rates of M. avium were found to be dependent upon the initial concentration of palmitic acid presented to the cells. The division constant varied from 0.05 to 0.13 when the concentration of palmitic acid ranged from 0 to 175 nmol/ml of medium. With ["4C ]palmitic acid as a tracer, it was found that rapid cell division began upon cessation of fatty acid uptake. During division, new lipid materials were released which contained "4C derived from ["4C]palmitic acid. Limited cell division and no fragmentation occurred in fatty acid-starved cultures. During fatty acid starvation, the transparent colony form, considered a pathogen, underwent a transition to the colony form considered a nonpathogen. The possible relationships between the organism's dependence on fatty acid and its ability to infect humans are discussed. 363 on August 5, 2020 by guest http://iai.asm.org/ Downloaded from 364 MCCARTHY on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Downloaded Frommentioning

confidence: 98%

Effect of Palmitic Acid Utilization on Cell Division in Mycobacterium avium

McCarthy

1974

Infect Immun

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Neueste Forschungsergebnisse über das Mycobacterium tuberculosis

Bassermann

1965

Beitr. Klin. Tuberk.

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Virulence and drug susceptibility of Mycobacterium celatum

Fattorini

Baldassarri

et al. 2000

Microbiology

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The virulence and drug susceptibility of a clinical isolate of Mycobacterium celatum which showed smooth transparent (ST) and smooth opaque (SO) colonies were studied. While ST cells multiplied intracellularly and maintained their coccobacillary form in a human macrophage model of infection, SO cells formed long filaments and completely destroyed the phagocytes. In BALB/c mice, the ST variant, but not the SO variant, grew efficiently in the spleen, liver and lung. The ST variant was usually more resistant in vitro than the SO variant to drugs, with MIC values for clarithromycin (CLA), azithromycin (AZI), ciprofloxacin, sparfloxacin, amikacin, clofazimine, ethambutol and isoniazid being higher than those of the SO variant. In beige mice infected with the more highly virulent variant ST, CLA and AZI were the most active drugs in terms of viable count reduction in organs and mutant selection. Together, these observations indicate that the ST variant of M. celatum is a virulent form that can be efficiently inhibited in vivo by CLA and AZI.

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GROWTH CHARACTERISTICS OF MYCOBACTERIUM AVIUM AND GROUP III NONPHOTOCHROMOGENIC MYCOBACTERIA IN HELA CELLS

Abstract: BROSBE, EDWIN A. (Veterans Administration Hospital, Long Beach, Calif.), PAUL T. SUGIIHARA, AND C. RICHARD SMITH. Growth characteristics of Mycobacterium avium and group III nonphotochromogenic mycobacteria in HeLa cells.

Cited by 5 publications

References 7 publications

Effect of Palmitic Acid Utilization on Cell Division in Mycobacterium avium

Effect of Palmitic Acid Utilization on Cell Division in Mycobacterium avium

Neueste Forschungsergebnisse über das Mycobacterium tuberculosis

Virulence and drug susceptibility of Mycobacterium celatum

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