Growth Characteristics of the Shay Chloroleukemia in Diffusion Chambers

Ferris, Philip; LoBue, Joseph; Gordon, Albert S.

doi:10.3181/00379727-133-34592

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…The present subline of Shay chloroleukaemia (see Rytomaa & Kiviniemi 1968, 1970, Vilpo 1972 has now been maintained for 3 years in inbred Wistar rats by transplanting 1-2 million cells subcutaneously or sometimes intraperitoneally.…”

Section: Chamber Cells Inoculatedmentioning

confidence: 96%

“…Similar observations were made with leukaemic 'Mast' cells of actinium-277-induced chloroma maintained in Sprague-Dawley rats when the cells were cultivated in diffusion chambers in the peritoneal cavity of rats (Parrish & Kleinfeld 1963). On the other hand, the morphological characteristics of Shay chloroleukaemia cells cultivated for 7 d in diffusion chambers in vivo were identical with those of the young tumour cells used to initiate the cultures (Ferris et al 1970). Similarly, no signs of distinct maturation or differentiation of Shay chloroleukaemia cells to polymorphonuclear cells appeared in 172 diffusion chamber cultures with culture periods varying from 1 to 56 d (Vilpo 1972).…”

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confidence: 93%

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Differentiation of Leukaemic Cells in Diffusion Chambers

1973

Scandinavian Journal of Haematology

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Morphological and cytochemical characteristics of cells obtained from Shay chloroleukaemia tumour growing subcutaneously in the rat and after 0.5–16 d of diffusion chamber culturing were assayed using seven different cytochemical procedures. Further, the cytochemical characteristics of the recent subline of Shay chloroma were compared with those reported in 1962 by other workers. The chloroleukaemia cells did undergo some maturation in diffusion chamber cultures, but full differentiation to polymorphonuclear cells was not seen. The most striking change in the cytochemical pattern of chloroleukaemia cells during the diffusion chamber culturing was a significant increase in alkaline phosphatase activity. There were some cytological differences in the recent chloroma tumour compared with that described 10 years ago. The observations made in the present work show that all properties of cells after a neoplastic transformation are not irreversible: many characteristics of malignant chloroleukaemia cells can alter when these cells are maintained in different host animals or in the diffusion chamber milieu.

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Section: Chamber Cells Inoculatedmentioning

confidence: 96%

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confidence: 93%

Differentiation of Leukaemic Cells in Diffusion Chambers

1973

Scandinavian Journal of Haematology

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Diffusion chamber colony‐forming unit (cfu‐d): A primitive stem cell

Niskanen¹,

Chatelain²,

Symann³

1989

The International Journal of Cell Cloning

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Assay of hematopoietic precursor cells in diffusion chambers (DCs) implanted intraperitoneally in experimental animals provides a powerful tool for studying stem cell kinetics in vivo. In this system, the effect of cell migration (which complicates whole animal studies) is eliminated because the membranes utilized in the construction of the chambers are impermeable for cells, while permitting free passage of molecules present in the humoral phase of the host. As judged by light microscopy, conditions in the DC cultures primarily favor macrophage and granulocyte growth. However, the use of in vitro and in vivo subculture to further analyze chamber contents has demonstrated that the system supports proliferation of early hematopoietic progenitors. Additionally, cells capable of rescuing lethally irradiated mice proliferate in DC cultures. Development of the plasma clot DC technique has revealed that most of the growth occurs in colonies which are derived from single cells (CFU-d). Characterization of these cells indicates that they are at least as primitive as other colony-forming cells and, also based on subculture studies, can differentiate along several hematopoietic lineages. In addition to normal CFU-d, both embryonal and leukemic cells can give rise to granulocytes, macrophages, megakaryocytes and erythroid cells in the DC cultures. Evaluation of the effects of humoral factors on hematopoietic cell proliferation and differentiation in the system has led to the identification of both stimulators and inhibitors that may be different from the well-characterized cytokines. Thus, the system seems to be useful for detecting molecules controlling the most primitive stages of hematopoiesis. We believe that the DC culture technique holds enormous potential in the study of stem cell proliferation and differentiation in vivo.

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Growth Characteristics of the Shay Chloroleukemia in Diffusion Chambers

Cited by 2 publications

References 4 publications

Differentiation of Leukaemic Cells in Diffusion Chambers

Differentiation of Leukaemic Cells in Diffusion Chambers

Diffusion chamber colony‐forming unit (cfu‐d): A primitive stem cell

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