Growth Characteristics of Two Rhinovirus Strains in WI-26 and Monkey Kidney Cells

Abstract

Viruses with 1059 and HGP serotype and with human and monkey host range characteristics, respectively, were employed. Adsorption kinetics of the 1059 and HGP strains to WI-26 cells, and HGP to Green African monkey kidney cells (MKC), were similar. Fifty per cent of the virus was adsorbed to ceU monolayers within 10 min; adsorption was essentially complete by 2 hr. The 1059 strain failed to adsorb to MKC, at least to an appreciable extent. Lack of receptors for adsorption of 1059 accounts for the inability of this cell to support multiplication of the virus. It is probable that MKC are refractory to infection with other H strains of rhinovirus for the same reason. Single-step multiplication cycles have been described for the HGP strain in WI-26 and MKC cultures and for the 1059 strain in WI-26 cells. In both cells, HGP exhibited a latent period of 7 hr. Increase of intracellular and cell-associated virus appeared somewhat prior to that of extracellular virus. Maximal titers were attained by 9 to 10 hr. In contrast, initial increase of 1059 in WI-26 cells occurred after 10 hr. Titer rose to peak level 15 hr after infection. Yield of 1059 in WI-26 cells was also fivefold lower than that of HGP in either cell system.

Rhinovirus Load Is High despite Preserved Interferon-β Response in Cystic Fibrosis Bronchial Epithelial Cells

“…In the short-term HRV inoculation model, we exposed cells to HRV16 at MOI of 0.1 for 2 hours at 37°C. This amount of time is sufficient for full absorption of low-titre HRV [ 19 – 21 ]. After that, cell supernatants were aspirated, and cells were washed twice with virus-free culture medium to remove any remaining extracellular virus.…”

Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA

“…In addition, we examined whether the dsRNA-HTRF assay could detect a single cycle of viral replication. Single-cycle growth of HRV is considered to take about 7 h [8]. H1-HeLa cells were infected with HRV-B14 at MOI of 10 to achieve single-cycle growth, and viral dsRNA was detected at 6 h post-infection using the dsRNA-HTRF assay ( Supplementary Fig.…”

The Common Cold

“…The na~opharyngeal secretions collected should be inoculated into several types of tissue cultures simultaneously to maximize yield (42,53,79,90,97,128). Each type of respiratory virus replicates best in certain tissue cultures (Table 7.3).…”