Growth Complementation of hrpXo Mutants of Xanthomonas oryzae pv. oryzae by Virulent Strains in Rice Cultivars Resistant and Susceptible to the Parental Strain

Tsuge, Seiji; Furutani, Ayako; Fukunaka, Rie; Kubo, Yasuyuki; Horino, Osamu

doi:10.1007/pl00012987

Cited by 27 publications

(15 citation statements)

References 22 publications

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“…Reactions were visualized using p-nitrophenyl phosphate (1 mg/ml) for ELISA and nitroblue tetrazolium (337.5 g/ml) and 5-bromo-4-chloro-3-indolyl phosphate (175 g/ml) for the immunoblot assay. Strain 74⌬HrpXo is a hrpXo-deficient mutant described previously (25). a Total RNA (10 ng) extracted from each bacterial strain after incubation in XOM2 was used as a template.…”

Section: Fig 2 Effects Of Mutation In Trh On Expression Of Hrpgmentioning

confidence: 99%

Gene Involved in Transcriptional Activation of the hrp Regulatory Gene hrpG in Xanthomonas oryzae pv. oryzae

et al. 2006

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Section: Fig 2 Effects Of Mutation In Trh On Expression Of Hrpgmentioning

confidence: 99%

Gene Involved in Transcriptional Activation of the hrp Regulatory Gene hrpG in Xanthomonas oryzae pv. oryzae

et al. 2006

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“…Using the resulting plasmid, marker-exchange mutagenesis of X. oryzae pv. oryzae strain MAFF311018 was conducted, 31) generating 74ÁXoo2875, in which þ25 to þ1156 of Xoo1488 (1,719 bp of total length) was replaced with the kanamycin resistance gene.…”

Section: Methodsmentioning

confidence: 99%

Suppression of Rice Immunity by Xanthomonas oryzae Type III Effector Xoo2875

Yamaguchi

Nakamura

Ishikawa

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…oryzae strain T7174, T7174R, has the same pathogenicity as the parental strain and was used as the wild type in this study (9,36). Mutant 74⌬HrpXo is an hrpXo-deficient mutant derived from T7174R (32). Strains of X. oryzae pv.…”

Section: Methodsmentioning

confidence: 99%

“…The plasmids were introduced into X. oryzae pv. oryzae by electroporation as described previously (32). Isolation of an orf1 mutant and an orf2 mutant of X. oryzae pv.…”

Section: Reverse Transcription Pcr (Rt-pcr)mentioning

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Effects on Promoter Activity of Base Substitutions in the cis -Acting Regulatory Element of HrpXo Regulons in Xanthomonas oryzae pv. oryzae

et al. 2005

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In Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, HrpXo is known to be a transcriptional regulator for the hypersensitive response and pathogenicity (hrp) genes. Several HrpXo regulons are preceded by a consensus sequence (TTCGC-N 15 -TTCGC), called the plant-inducible promoter (PIP) box, which is required for expression of the gene that follows. Thus, the PIP box can be an effective marker for screening HrpXo regulons from the genome database. It is not known, however, whether mutations in the PIP box cause a complete loss of promoter activity. In this study, we introduced base substitutions at each of the consensus nucleotides in the PIP box of the hrpC operon in X. oryzae pv. oryzae, and the promoter activity was examined by using a ␤-glucuronidase (GUS) reporter gene. Although the GUS activity was generally reduced by base substitutions, several mutated PIP boxes conferred considerable promoter activity. In several cases, even imperfect PIP boxes with two base substitutions retained 20% of the promoter activity found in the nonsubstituted PIP box. We screened HrpXo regulon candidates with an imperfect PIP box obtained from the genome database of X. oryzae pv. oryzae and found that at least two genes preceded by an imperfect PIP box with two base substitutions were actually expressed in an HrpXo-dependent manner. These results indicate that a base substitution in the PIP box is quite permissible for HrpXo-dependent expression and suggest that X. oryzae pv. oryzae may possess more HrpXo regulons than expected.Many gram-negative, plant-pathogenic bacteria possess clustered hypersensitive response and pathogenicity (hrp) genes. These genes encode proteins involved in the type III secretion system that delivers effector proteins from the bacteria to plant cells. These proteins are required for pathogenesis in host plants and for triggering a hypersensitive response (HR) in nonhost plants (reviewed in references 1, 6, and 41).The expression of hrp genes is highly regulated. These genes are induced only in plants or certain nutrient-poor synthetic media and are not induced in nutrient-rich complex media (3,16,20,30,31,33,40). There are two types of hrp regulatory systems in plant-pathogenic bacteria (1, 14). In group I systems, which are found in Pseudomonas syringae, Erwinia sp., and Pantoea stewartii, a member of the ECF family of alternative sigma factors, called HrpL, functions as the regulator for other hrp genes (21,42,43). On the other hand, in group II systems, which are found in Xanthomonas sp. and Ralstonia solanacearum, either the AraC-type transcriptional activator HrpX (Xanthomonas sp.) or HrpB (R. solanacearum) regulates expression of other hrp genes (13,29,39).In xanthomonads, the hrp cluster comprises six hrp loci, hrpA to hrpF, which encode components of a type III secretion system, and all of these genes are required for full pathogenicity (5). Two regulatory genes that control expression of the hrp genes have been identified in the genus Xanthomonas.

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Growth Complementation of hrpXo Mutants of Xanthomonas oryzae pv. oryzae by Virulent Strains in Rice Cultivars Resistant and Susceptible to the Parental Strain

Cited by 27 publications

References 22 publications

Gene Involved in Transcriptional Activation of the hrp Regulatory Gene hrpG in Xanthomonas oryzae pv. oryzae

Gene Involved in Transcriptional Activation of the hrp Regulatory Gene hrpG in Xanthomonas oryzae pv. oryzae

Suppression of Rice Immunity by Xanthomonas oryzae Type III Effector Xoo2875

Effects on Promoter Activity of Base Substitutions in the cis -Acting Regulatory Element of HrpXo Regulons in Xanthomonas oryzae pv. oryzae

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