Growth Conditions for Plant Virus–Host Studies

Abstract

Plant virus replication and spread require specific interactions between the obligate organism, the virus, and its host, the plant. Both virus and plant factors, whether they are involved in virus accumulation or in defense against virus accumulation, require specific environmental conditions to maximize their activity. In order to understand how virus and plant factors function, it is therefore necessary to understand and control the environment that influences their activity. Temperature, light, moisture, air movement, soil status, and plant nutrition are the major environmental factors that influence a virus' accumulation in its host. This unit discusses each of these environmental factors, documenting their influence on virus accumulation in the plant, and discusses methods to best control and monitor their variation. Controlling the environment will limit experimental variation and lead to more meaningful and reproducible experimental results. Curr. Protoc. Microbiol. 14:16A.1.1‐16A.1.16. © 2009 by John Wiley & Sons, Inc.

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“…This showed that the symptoms that appear on plants infected by specific isolates can be visually different. Plant and environmental conditions also influence disease symptoms due to pathogen infestation (Liu et al, 2009). Both isolates caused changes in the leaves of melon and bitter melon plants, namely the blanching of the lamina and the formation of striped patterns on the plant leaves.…”

Impact of Concurrent Drought Stress and Pathogen Infection on Plants

“…Drought has also been shown to negatively affect virus translocation in plants (Liu et al 2009). For example, drought inhibits the systemic spread of tomato spotted wilt virus in tomato (Cordoba et al 1991).…”

Lower temperature influences Cauliflower mosaic virus systemic infection

“…Plant viral infections are influenced by a variety of environmental factors (Kassanis 1957;Liu et al 2009). However, many questions remain regarding the relationship between temperature, virus propagation, and host-responses (Honjo et al 2020).…”