Growth cones of regenerating adult sciatic sensory axons release axonally transported proteins

Remgård, Pär; Edbladh, M.; Ekström, Per; Edström, Anders

doi:10.1016/0006-8993(92)90462-i

Cited by 5 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, is axonal protein synthesis essential for any axonal function? There are conflicting reports for axonal growth: inhibition of axonal translation has been reported to inhibit axonal regeneration in rodent nerve (Remgård et al, 1992;Edbladh et al, 1994;Gaete et al, 1998) but to have no effect on the growth rate of cultured rat sympathetic neurons (Eng et al, 1999). However, recent reports show that the response to guidance cues of growth Many axons carry out the synthesis of macromolecules independent of their cell bodies but the nature, organization and magnitude of axonal protein synthesis remain unclear.…”

mentioning

confidence: 99%

Organization and translation of mRNA in sympathetic axons

Lee

Hollenbeck

2003

Journal of Cell Science

View full text Add to dashboard Cite

Many axons carry out the synthesis of macromolecules independent of their cell bodies but the nature, organization and magnitude of axonal protein synthesis remain unclear. We have examined these features in axons of chick sympathetic neurons in cell culture. In situ hybridization showed that poly(A) mRNA is abundant and non-uniformly distributed in nearly all axons. The specific transcripts for β-actin and actin-depolymerizing factor (ADF) were also present and non-uniformly distributed in axons, with an approximately hundredfold higher concentration in growth cones, branch points and axonal varicosities than in the axon shaft. Immunoprecipitation using specific antibodies indicates that β-actin, ADF and neurofilament protein (NF) are translated in axons independently of cell bodies. Quantification of the distribution of β-actin and ADF mRNAs showed that their ability to enter the axon was likely to be a property of the neuron as a whole rather than of individual axons. To compare the distribution of axonally translated protein to that of mRNA, we performed 35S metabolic labeling with axons separated from their cell bodies. Axonally synthesized proteins were distributed throughout the axons and their synthesis was inhibited by cycloheximide but not by chloramphenicol. Proteins translated mainly or exclusively in axons or cell bodies were both detected by metabolic labeling. Axons separated from their cell bodies synthesized up to 5% as much protein in a 3-hour period as did intact neurons. Because axons in our culture conditions contain ∼50% of the non-nuclear volume of the neurons, we estimate that axoplasm of sympathetic neurons has a protein synthetic capacity per unit volume equal to 10% that of cell body cytoplasm.

show abstract

mentioning

confidence: 99%

Organization and translation of mRNA in sympathetic axons

Lee

Hollenbeck

2003

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…5 watchmaker's forceps. After 4 days of regeneration in vivo, the sciatic nerves together with dorsal root ganglia 9 and 10 were dissected out and incubated in a multicompartment chamber, which allowed the ganglia and outgrowth region to be kept well separated from each other (Remgird et al, 1992). In brief, rings were cut from polystyrene test tubes, and the nerve was fitted through a small notch made at the bottom of each ring.…”

Section: Nerve Preparationsmentioning

confidence: 99%

Regenerating Peripheral Axons Transport and Release Low‐Molecular‐Mass Materials In Vitro

Remgård

Ekström

Edström

1994

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

The release of radiolabeled material from regenerating frog sciatic nerves was studied using a multicompartment chamber, in which the ganglia and the outgrowth region, respectively, were separated from the rest of the nerve. The nerves were incubated with radioactive amino acids in the ganglionic compartment, and the material transported to and released at the outgrowth region was collected and analyzed. Approximately 10% of the transported radioactivity was released over a 24-h incubation period. Of the released materials, 84% had a molecular mass of t1,OOO daltons [the low-molecular-mass (LM)

show abstract

Release of slow axonally transported proteins from the rat vagus nerve in vitro

Remgård

Kanje

1994

Brain Research

View full text Add to dashboard Cite

Growth cones of regenerating adult sciatic sensory axons release axonally transported proteins

Cited by 5 publications

References 17 publications

Organization and translation of mRNA in sympathetic axons

Organization and translation of mRNA in sympathetic axons

Regenerating Peripheral Axons Transport and Release Low‐Molecular‐Mass Materials In Vitro

Release of slow axonally transported proteins from the rat vagus nerve in vitro

Contact Info

Product

Resources

About