Growth detection of Cutibacterium acnes from orthopaedic implant-associated infections in anaerobic bottles from BACTEC and BacT/ALERT blood culture systems and comparison with conventional culture media

Jeverica, Samo; Sayed, Faten El; Camernik, P.; Kocjančič, Boštjan; Sluga, Boštjan; Rottman, Martin; Papst, Lea

doi:10.1016/j.anaerobe.2019.102133

Cited by 22 publications

(18 citation statements)

References 28 publications

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“…The blood culture vial used was the Lytic Ana vial, proven for its effectiveness in this setting (6,7). Nearly 40 cases of infection and contamination with C. acnes from our center were included, making it one of the largest clinical series published to date (10-12).…”

Section: Discussionmentioning

confidence: 99%

“…This is the first study to examine the relationship between C. acnes genotype and the time to detection in blood culture vials seeded with intraoperative samples from orthopedic surgery. The blood culture vial used was the Lytic Ana vial, proven for its effectiveness in this setting (6, 7). Nearly 40 cases of infection and contamination with C. acnes from our center were included, making it one of the largest clinical series published to date (10–12).…”

Section: Discussionmentioning

confidence: 99%

“…Our data address solely the Lytic Ana vial and other studies are necessary to determine whether they can be extrapolated to other blood culture media routinely used for the enrichment of bone and joint samples. We have previously reported evidence that each blood culture medium formulation has specific growth characteristics for C. acnes (7). The prolonged incubation of liquid media for the enhanced recovery of C. acnes has been widely recommended, regardless of its diagnostic value.…”

Section: Discussionmentioning

confidence: 99%

“…"Orthopedic" strains inoculated in vitro into anaerobic BCBs were recently found to be associated with a broad range of TTD values (7). This prompted us to study whether such variability may be related to the genotype and to evaluate its clinical impact.…”

Section: Introductionmentioning

confidence: 99%

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Cutibacterium acnesclonal complexes display various growth rates in blood-culture bottles used for diagnosing orthopedic device-related infections

Sayed

Camernik

Roux

et al. 2021

Preprint

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Blood-culture bottles (BCBs) are widely used to improve the diagnosis of orthopedic device-related infections. Data is scarce on the growth of Cutibacterium acnes and its genotypes in BCBs under real-life clinical conditions.We studied 39 cases of revision arthroplasty for which at least one intraoperative sample yielded a pure C. acnes culture from anaerobic BCBs (BD Bactec Lytic/10 Anaerobic/F [Lytic Ana]) and/or solid media. Genotyping of C. acnes isolates from the 39 cases allowed: i) the identification of 49 non-redundant isolates belonging to four clonal complexes (CCs): CC18, CC28, CC36, and CC53 and ii) the determination of infectant and contaminant strains. Under real-life clinical conditions, Lytic Ana alone was more often positive for contaminants than infectant strains (18/36 [50%] versus 2/13 [15.4%]; p = 0.047). The time to detection (TTD) values in Lytic Ana were shorter for CC53 than other CCs (mean [SD] TTD: 77 [15] versus 165 [71] hours; p = 0.02). CC53 was confirmed to grow faster than other CCs by studying an enlarged panel of 70 genotyped C. acnes strains inoculated in vitro into Lytic Ana vials (mean [SD] TTD: 73 [13] versus 122 [50] hours; p < 0.001).The use of Lytic Ana BCBs in orthopedics increases the recovery rate of C. acnes but leads to the isolation of proportionally more contaminants than true infectant strains. TTD values are much shorter for CC53 strains, irrespective of their being infectant or contaminant. TTD does not solely reflect the bacterial load of samples but also clonal complex-related traits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cutibacterium acnesclonal complexes display various growth rates in blood-culture bottles used for diagnosing orthopedic device-related infections

Sayed

Camernik

Roux

et al. 2021

Preprint

Self Cite

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show abstract

“…mainly originate from the gastrointestinal tract, GPAC, pigmented Prevotella spp., Porphyromonas spp., and Fusobacterium spp. arise from the upper airways, from pulmonary sources or the female genital tract, while Cutibacterium acnes mainly originated from the skin and foreign bodies [ 1 , 2 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Relevance of anaerobic bacteremia in adult patients: A never-ending story?

Gajdács

Urbán

2020

European Journal of Microbiology and Immunology

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Abstract Obligate anaerobic bacteria are considered important constituents of the microbiota of humans; in addition, they are also important etiological agents in some focal or invasive infections and bacteremia with a high level of mortality. Conflicting data have accumulated over the last decades regarding the extent in which these pathogens play an intrinsic role in bloodstream infections. Clinical characteristics of anaerobic bloodstream infections do not differ from bacteremia caused by other pathogens, but due to their longer generation time and rigorous growth requirements, it usually takes longer to establish the etiological diagnosis. The introduction of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has represented a technological revolution in microbiological diagnostics, which has allowed for the fast, accurate and reliable identification of anaerobic bacteria at a low sample cost. The purpose of this review article is to summarize the currently available literature data on the prevalence of anaerobic bacteremia in adults for physicians and clinical microbiologists and to shed some light on the complexity of this topic nowadays.

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Approaches to the Identification of Anaerobic Bacteria

Boyanova,

Medeiros

2023

ClinMicroNow

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This chapter focuses on important requirements of anaerobic microbiology and methods and techniques used in the diagnostic workflow. Anaerobic bacteria (anaerobes) can cause infections in all tissues and organs of the human body. The chapter summarizes some important recommendations for specimen collection and handling for diagnosing anaerobic infections. Moreover, since antibiotic resistance in anaerobes and the proportion of patients with immunosuppressive diseases or immunosuppressive therapy are constantly increasing, anaerobic microbiology is becoming increasingly important and must not be neglected in any hospital center or country. The disk diffusion method may facilitate susceptibility testing of anaerobes. However, all microbiology laboratories should, at the minimum, be able to culture and perform basic identification of anaerobes and refer uncommon isolates to a reference laboratory. Both clinicians and laboratory staff should be familiar with the requirements for collection and transport of clinical samples for anaerobic microbiology.

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Growth detection of Cutibacterium acnes from orthopaedic implant-associated infections in anaerobic bottles from BACTEC and BacT/ALERT blood culture systems and comparison with conventional culture media

Cited by 22 publications

References 28 publications

Cutibacterium acnesclonal complexes display various growth rates in blood-culture bottles used for diagnosing orthopedic device-related infections

Cutibacterium acnesclonal complexes display various growth rates in blood-culture bottles used for diagnosing orthopedic device-related infections

Relevance of anaerobic bacteremia in adult patients: A never-ending story?

Approaches to the Identification of Anaerobic Bacteria

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