Growth Enhancement of Probiotic Pediococcus acidilactici by Extractive Fermentation of Lactic Acid Exploiting Anion-Exchange Resin

Abstract

Fermentation employing lactic acid bacteria (LAB) often suffers end-product inhibition which reduces the cell growth rate and the production of metabolite. The utility of adsorbent resins for in situ lactic acid removal to enhance the cultivation performance of probiotic, Pediococcus acidilactici was studied. Weak base anion-exchange resin, Amberlite IRA 67 gave the highest maximum uptake capacity of lactic acid based on Langmuir adsorption isotherm (0.996 g lactic acid/g wet resin) compared to the other tested anion-exchange resins (Amberlite IRA 410, Amberlite IRA 400, Duolite A7 and Bowex MSA). The application of Amberlite IRA 67 improved the growth of P. acidilactici about 67 times compared to the control fermentation without resin addition. Nevertheless, the in situ addition of dispersed resin in the culture created shear stress by resins collision and caused direct shear force to the cells. The growth of P. acidilactici in the integrated bioreactor-internal column system containing anion-exchange resin was further improved by 1.4 times over that obtained in the bioreactor containing dispersed resin. The improvement of the P. acidilactici growth indicated that extractive fermentation using solid phase is an effective approach for reducing by-product inhibition and increasing product titer.

In situ solid-liquid extraction enhances recovery of taxadiene from engineered S. cerevisiae cell factories

“…Such degradation was likely enhanced by culture agitation as it has been noted in previous works using similar type of resins [35]. Furthermore, as the resin beads are around 100-fold larger than the yeast cells, their presence within the culture likely contributed to shear stress, which may have hindered cell viability at the higher bead concentrations (>6 % (w/v)).…”

Application of PLGA-ion exchange resin microcapsules of sulfasalazine ‎for embolization therapy

“…The results showed a significant increase in encapsulation effi ciency and percent of yield by increasing the volume of aqueous phase that may extract the solvent instantly which lead to fast precipitation of the coating polymer (PLGA) in large volume of continuous phase, 28 as shown in Fig. 3.…”

Fermentative Lactic Acid Production From Lignocellulosic Feedstocks: From Source to Purified Product

“…Integration of fermentation and separation steps in situ extractive fermentation gives the possibility of increasing productivity and decreasing energy consumption. Different methods are proposed for lactic acid removal-ion exchange (Othman et al, 2018), membrane bioreactors (Gössi et al, 2020), liquid-liquid extraction (Lan et al, 2019). Although process integration is well documented in the case of lactic acid production from sugars (M. Othman et al, 2017), there are no published results for second-generation biomass.…”