Growth enhancing effect of LBL-assembled magnetic nanoparticles on primary bone marrow cells

Li, Xuan; Zhang, Jie; Tang, Shijia; Sun, Jianfei; Lou, Zhichao; Yang, Yan; Wang, Peng; Li, Yan; Gu, Ning

doi:10.1007/s40843-016-5104-9

Cited by 22 publications

(25 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assembly of γ-Fe 2 O 3 nanoparticles reduced the collective free energy of nanoparticles while augmenting the magnetic energy. This hypothesis can be partly supported by our conclusions in previous reports that assemblies of magnetic nanoparticles can mediate the magnetic effect on cells [13,25]. Here, we measured the magnetosensing protein by q-PCR, which was recently reported to be capable of responding to magnetic interaction (Fig.…”

Section: $57and/(6 Science China Materialssupporting

confidence: 83%

See 1 more Smart Citation

Uptake of magnetic nanoparticles for adipose-derived stem cells with multiple passage numbers

et al. 2017

Self Cite

View full text Add to dashboard Cite

With the increasingly promising role of nanomaterials in tissue engineering and regenerative medicine, the interaction between stem cells and nanoparticles has become a critical focus. The entry of nanoparticles into cells has become a primary issue for effectively regulating the subsequent safety and performance of nanomaterials in vivo. Although the influence of nanomaterials on endocytosis has been extensively studied, reports on the influence of stem cells are rare. Moreover, the effect of nanomaterials on stem cells is also dependent upon the action mode. Unfortunately, the interaction between stem cells and assembled nanoparticles is often neglected. In this paper, we explore for the first time the uptake of γ-Fe 2 O 3 nanoparticles by adipose-derived stem cells with different passage numbers. The results demonstrate that cellular viability decreases and cell senescence level increases with the extension of the passage number. We found the surface appearance of cellular membranes to become increasingly rough and uneven with increasing passage numbers. The iron content in the dissociative nanoparticles was also significantly reduced with increases in the passage number. However, we observed multiple-passaged stem cells cultured on assembled nanoparticles to have similarly low iron content levels. The mechanism may lie in the magnetic effect of γ-Fe 2 O 3 nanoparticles resulting from the field-directed assembly. The results of this work will facilitate the understanding and translation of nanomaterials in the clinical application of stem cells.

show abstract

Section: $57and/(6 Science China Materialssupporting

confidence: 83%

“…Recently, magnetic nanoparticles have shown an increasingly promising role in the construction of novel scaffolds that can impose local magnetic, thermal, and mechanic stimuli [12][13][14]. As such, tissue growth can be accelerated and some small molecules or cytokines can be released in a controlled way [15].…”

Section: Introductionmentioning

confidence: 99%

Uptake of magnetic nanoparticles for adipose-derived stem cells with multiple passage numbers

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, gene expression of an exogenous magnetoreceptor, an iron-sulfur cluster assembly protein 1 (ISCA1), was examined to confirm the results. An up-regulated expression of ISCA1 would be found if magnetism had significant effects [44]. However, there were no significant differences in its expression among CPC control, cIONP-CPC and DcIONP-CPC (p > .05, Figure S7(e)).…”

Section: Effects Of Magnetism In Enhancing Cellular Osteogenic Differmentioning

confidence: 92%

Injectable calcium phosphate scaffold with iron oxide nanoparticles to enhance osteogenesis via dental pulp stem cells

Xia

Chen

Zhang

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

View full text Add to dashboard Cite

Literature search revealed no systematic report on iron oxide nanoparticle-incorporating calcium phosphate cement scaffolds (IONP-CPC). The objectives of this study were to: (1) use γFeO nanoparticles (γIONPs) and αFeO nanoparticles (αIONPs) to develop novel IONP-CPC scaffolds, and (2) investigate human dental pulp stem cells (hDPSCs) seeding on IONP-CPC for bone tissue engineering for the first time. IONP-CPC scaffolds were fabricated. Physiochemical properties of IONP-CPC scaffolds were characterized. hDPSC seeding on scaffolds, cell proliferation, osteogenic differentiation and bone matrix mineral synthesis by cells were measured. Our data demonstrated that the osteogenic differentiation of hDPSCs was markedly enhanced via IONP incorporation into CPC. Substantial increases (about three folds) in ALP activity and osteogenic gene expressions were achieved over those without IONPs. Bone matrix mineral synthesis by the cells was increased by two- to three folds over that without IONPs. The enhanced cellular osteogenesis was attributed to: (1) the surface nanotopography of IONP-CPC scaffold, and (2) the cell internalization of IONPs released from IONP-CPC scaffold. Our results demonstrate that the novel CPC functionalized with IONPs is promising to promote osteoinduction and bone regeneration. In conclusion, it is highly promising to incorporate γIONPs and αIONPs into CPC scaffold for bone tissue engineering, yielding substantially better stem cell attachment, spreading and osteogenic differentiation, and much greater bone mineral synthesis by the seeded cells. Therefore, novel CPC scaffolds containing γIONPs and αIONPs are promising for dental, craniofacial and orthopaedic applications to substantially enhance bone regeneration.

show abstract

“…We have fabricated the macroscopic film of IONPs by layer‐by‐layer (LBL) assembly on PLA scaffold, and studied the effect of the assembled IONPs film on BMCs . The results showed that the LBL‐assembled film could promote the growth and osteogenic differentiation of cells, especially combined with the external magnetic field.…”

Section: Iopns‐based Composite Bone Scaffoldsmentioning

confidence: 99%

Adaptive Materials Based on Iron Oxide Nanoparticles for Bone Regeneration

et al. 2018

ChemPhysChem

Self Cite

View full text Add to dashboard Cite

The paper provides a brief overview of the use of iron oxide nanoparticles (IONPs) in the areas of bone regenerative medicine. Reconstruction of bone defects caused by trauma, non-union, and bone tumor excision, still faces many challenges despite the intense investigations and advancement in bone-tissue engineering and bone regeneration over the past decades. IONPs have promising prospects in this field due to their controlled responsive characteristics in specific external magnetic fields and have been of great interest during the last few years. This Minireview aims to summarize the relevant progress and describes the following five aspects: (i) The general introduction of IONPs, with a focus on the magnetic properties as the base of application; (ii) using IONPs as tools to study and control stem cells for better treatment efficacy in stem-cell-based bone defect repair; (iii) the use of IONPs and their complexes in the delivery of therapeutic agents, including chemical drug molecules, growth factors, and genetic materials, to promote osteogenesis-related cell function and differentiation, healthy bone tissue growth, and functional reconstruction; (iv) magneto-mechanical actuation in the regulation of cells distribution, mechano-transduction membrane receptors activation, and mechanosensitive signaling pathways regulation, and (v) fabrication, characteristics, and in vitro and in vivo osteogenic effects of magnetic composite bone scaffolds. Ongoing prospects are also discussed.

show abstract

Growth enhancing effect of LBL-assembled magnetic nanoparticles on primary bone marrow cells

Cited by 22 publications

References 32 publications

Uptake of magnetic nanoparticles for adipose-derived stem cells with multiple passage numbers

Uptake of magnetic nanoparticles for adipose-derived stem cells with multiple passage numbers

Injectable calcium phosphate scaffold with iron oxide nanoparticles to enhance osteogenesis via dental pulp stem cells

Adaptive Materials Based on Iron Oxide Nanoparticles for Bone Regeneration

Contact Info

Product

Resources

About