2021
DOI: 10.1016/j.bbiosy.2021.100009
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Growth factor and macromolecular crowding supplementation in human tenocyte culture

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Cited by 17 publications
(15 citation statements)
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“…Recent studies have argued that MMC may enable the accelerated development of functional tissue engineered medicines [ [34] , [35] , [36] ]. Indeed, in both hTC [ [37] , [38] , [39] , [40] ] and hDF [ [41] , [42] , [43] ] cultures, MMC has been shown to enhance (up to 120-fold) and accelerate (from 2 to 6 days) ECM deposition and to maintain hTC phenotype alone or in combination with other in vitro microenvironment modulators. It is also worth noting that the therapeutic potential of MMC has been demonstrated in preclinical setting [ 44 ], paving the way for its clinical translation.…”
Section: Introductionmentioning
confidence: 99%
“…Recent studies have argued that MMC may enable the accelerated development of functional tissue engineered medicines [ [34] , [35] , [36] ]. Indeed, in both hTC [ [37] , [38] , [39] , [40] ] and hDF [ [41] , [42] , [43] ] cultures, MMC has been shown to enhance (up to 120-fold) and accelerate (from 2 to 6 days) ECM deposition and to maintain hTC phenotype alone or in combination with other in vitro microenvironment modulators. It is also worth noting that the therapeutic potential of MMC has been demonstrated in preclinical setting [ 44 ], paving the way for its clinical translation.…”
Section: Introductionmentioning
confidence: 99%
“…We attribute this to the synergistic effect of the contained growth factors with MMC. Indeed, MMC combined with growth factor supplementation resulted in amplified (over cells with growth factors alone and cells with MMC alone) collagen deposition, as the growth factors enhanced collagen synthesis and MMC enhanced collagen deposition ( Tsiapalis et al, 2021 ). Following the same reasoning, one would have expected the ACF with MMC to induce higher collagen deposition than the ACF alone, which was not the case, as judged by SDS-PAGE.…”
Section: Discussionmentioning
confidence: 99%
“…Similarly, the presence of tissue-specific ECM has been shown to facilitate cell phenotype maintenance in vitro ( Pei et al, 2011 ; Cheng et al, 2014 ; Gattazzo et al, 2014 ; Yang et al, 2018a ). In eukaryotic cell culture systems, macromolecular crowding (MMC), following the principles of excluded volume effect, enhances and accelerates tissue-specific ECM deposition ( Raghunath and Zeugolis, 2021 ; Tsiapalis and Zeugolis, 2021 ; Zeugolis, 2021 ), a phenomenon that has been well documented in both differentiated ( Lareu et al, 2007 ; Satyam et al, 2014 ; Kumar et al, 2015a ; Kumar et al, 2015b ; Satyam et al, 2016 ; Kumar et al, 2018 ; Gaspar et al, 2019 ; Shendi et al, 2019 ; Tsiapalis et al, 2021 ) and progenitor cell cultures ( Zeiger et al, 2012 ; Prewitz et al, 2015 ; Cigognini et al, 2016 ; Lee et al, 2016 ; Patrikoski et al, 2017 ; Graceffa and Zeugolis, 2019 ; De Pieri et al, 2020 ). However, to-date, only one study has assessed the influence of MMC in xeno-free and/or serum-free media formulations using human adipose-derived mesenchymal stromal cells ( Patrikoski et al, 2017 ).…”
Section: Introductionmentioning
confidence: 99%
“…This is much lower than that in vivo, and can cause cells to undergo epigenetic changes, leading to altered protein and gene pro les, phenotypic drift, loss of function and senescence. As a result, this can't provide an ideal cellular model for vascular diseases research such as hypertension (7,14). Adding inert polydisperse macromolecules to the culture medium can simulate the in vivo crowding environment (15).…”
Section: Introductionmentioning
confidence: 99%