Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells <i>in vitro</i>

Abstract

Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.

Hyaluronic Acid Hydrogel Integrated with Mesenchymal Stem Cell‐Secretome to Treat Endometrial Injury in a Rat Model of Asherman's Syndrome

“…Numerous studies have indicated that stem cells exert their therapeutic benefits mainly through paracrine signaling . Secretome has shown immense potential to treat injured tissues, including injured endometrium 7a,9b,12a,15,29. Thus, MSC‐Sec could be an ideal candidate to treat endometrial injury.…”

“…To test the effect of different treatments for the injured uterus, an injured rat uterus model was induced by using electrocoagulation ( Figure A) . Electrocoagulation injures the endometrium of the uterus and reduces angiogenesis therein, simulating the pathological changes that lead to AS . Figure B shows the hematoxylin and eosin (H&E) and trichrome stained micrographs of the uteri before and after injury.…”

The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice

“…Several studies have reported that MSCs can be differentiated into granulosa cells and reduce apoptosis of granulosa cells so that MSCs can repair ovarian function for women after chemotherapy. MSCs can also provide a variety of cytokines and chemokines, including epidermal growth factor (EGF), insulin-like growth factor binding protein (IGFBP), and insulin-like growth factor-1 (IGF), for target cells through paracrine processes (Islam, et al 2016;Zhang, et al 2016). Several studies have suggested that MSCs stimulate the expression of anti-inflammatory cytokines, such as IL10 and tumor necrosis factor (TNF)-α, which improve the function of damaged fallopian tubes by decreasing the level of inflammatory factors (Li, et al 2017;Liao, et al 2019).…”

miR‐182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro

“…What's more, HOXA10 was regulated by many factors in EECs, including sex hormones (Taylor, Arici, Olive, & Igarashi, ; Wu et al., ; Zhong, Wang, & Liu, ), conceptus secretions (Blitek, Morawska, Kiewisz, & Ziecik, ) under normal physiological conditions. More importantly, variety of factors participated in the regulation of EECs under normal physiological conditions, including E2 (Shan, Li, Yang, & Li, ), P4 (Kyo et al., ), integrins (Park et al., ), periostin (Zheng et al., ), non‐esterified fatty acids (Chankeaw et al., ), growth factor (Islam, Yamagami, Yoshii, & Yamauchi, ), brain‐derived neurotrophic factor (Lim, Bae, Bazer, & Song, ). Moreover, HOXA10 was regulated by multiple miRNAs, such as miR‐135 in endometriosis (Petracco et al., ), miR‐494 in oral cancer (Libóriokimura, Jung, & Chan, ), miR‐218 in hepatocellular carcinoma cell (Zhong‐Di Xiao et al., ).…”