Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment

Liebesny, Paul; Byun, Sung Su; Hung, Han-Hwa; Pancoast, James R.; Mroszczyk, Keri; Young, Whitney; Lee, Richard T.; Frisbie, David D.; Kisiday, John D.; Grodzinsky, Alan J.

doi:10.1089/ten.tea.2015.0524

Cited by 24 publications

(24 citation statements)

References 92 publications

(124 reference statements)

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“…Similarly, in an in vitro rabbit model this combination also showed superior chemotactic effects on MSCs compared with either growth factors alone . The authors believe this is the first example of an in vivo application of these growth factors in a cartilage repair model, which supports our in vitro work …”

Section: Discussionsupporting

confidence: 82%

“…Our group has reported that HB‐IGF‐1 is strongly retained in articular cartilage post‐intra‐articular injection and leads to sustained proteoglycan biosynthesis in cartilage explants . Interestingly, an in vitro kinetics study showed that 84% of the HB‐IGF‐1 that was premixed into a KLD hydrogel was released within the first four days of culture; however, this HB‐IGF‐1 seems to be bound by surrounding cartilage annuli instead of being released in the media. Further studies are necessary to elucidate the lack of effect in the present study.…”

Section: Discussionmentioning

confidence: 67%

“…Self‐assembling peptide hydrogel scaffold has shown promise in augmenting microfracture . The self‐assembling capacity allows this product to be injected arthroscopically, and its high water content allows the cells to migrate and deposit extracellular matrix.…”

mentioning

confidence: 99%

“…Recently, platelet‐derived growth factor (PDGF) and transforming growth factor‐B1 (TGF‐B1) have been shown to increase MSC migration into the self‐assembling KLD hydrogel. In this same study, heparin‐binding insulin‐like growth factor‐1 (HB‐IGF‐1) stimulated extracellular matrix (ECM) deposition within the scaffold and in surrounding cartilage . We sought to assess the improved healing with these growth factors through delivery with self‐assembling KLD hydrogel.…”

mentioning

confidence: 99%

“…Self-assembling peptide hydrogel scaffold has shown promise in augmenting microfracture. 4 The self-assembling capacity allows this product to be injected arthroscopically, and its high water content allows the cells to migrate and deposit extracellular matrix. Self-assembling KLD hydrogel has proven to be biocompatible and stimulate cartilage repair by improving defect filling and increasing in tissue properties.…”

mentioning

confidence: 99%

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Trypsin Pre‐Treatment Combined With Growth Factor Functionalized Self‐Assembling Peptide Hydrogel Improves Cartilage Repair in Rabbit Model

Zanotto

Liebesny

Barrett

et al. 2019

Journal Orthopaedic Research

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The objective of this study was to improve cartilage repair and integration using self‐assembling KLD hydrogel functionalized with platelet‐derived growth factor‐BB and heparin‐binding insulin‐like growth factor‐1 with associated enzymatic trypsin pre‐treatment of the native cartilage. Bilateral osteochondral defects were created at the central portion of the femoral trochlear groove of 48 skeletally mature, white New Zealand rabbits. One limb received a randomly assigned treatment and the contralateral limb served as the control. Treated defects were exposed to trypsin for 2 min and filled with self‐assembling KLD hydrogel only, or associated to growth factors. All control limbs received KLD hydrogel alone or received only trypsin but not hydrogel. Ninety days post‐defect creation, the rabbits were euthanized and magnetic resonance imaging, radiography, macroscopic evaluation, histology, and immunohistochemistry of the joint and repaired tissue were performed. Mixed model analyses of variance were utilized to assess the outcome parameters and individual comparisons were performed using Least Square Means procedure and differences with p‐value < 0.05 were considered significant. Trypsin enzymatic pre‐treatment improved cellular morphology, cluster formation and subchondral bone reconstitution. Platelet‐derived growth factor‐BB improved subchondral bone healing and basal integration. Heparin‐binding insulin‐like growth factor‐1 associated with platelet‐derived growth factor improved tissue and cell morphology. The authors conclude that self‐assembling KLD hydrogel functionalized with platelet‐derived growth factor and heparin‐binding insulin‐like growth factor‐1 with associated enzymatic pre‐treatment of the native cartilage with trypsin resulted in an improvement on the cartilage repair process. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2307–2315, 2019

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 67%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Trypsin Pre‐Treatment Combined With Growth Factor Functionalized Self‐Assembling Peptide Hydrogel Improves Cartilage Repair in Rabbit Model

Zanotto

Liebesny

Barrett

et al. 2019

Journal Orthopaedic Research

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Advanced Biotechnologies Toward Engineering a Cell Home for Stem Cell Accommodation

Tian

et al. 2017

Adv Materials Technologies

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Biomaterials that exhibit bioinspired behavior enable a range of novel biomedical applications. Cell‐friendly biomaterials incorporating crucial physicochemical cues can instruct and dictate cell behavior both in vitro and in vivo. Despite substantial advances, engineering a complex cell regulatory system that incorporates mechanical and chemical signals targeting specific intracellular events has remained far from a reality. Recently, biological studies have begun to decipher the dynamic interplay between stem cells and their microenvironments. Moreover, the myriad recently developed advanced material biotechnologies, such as 3D bioprinting, protein engineering, release technology, high‐throughput screening, and microfluidics, have enabled the engineering of sophisticated biomaterial systems that increasingly closely mimic the spatial and temporal characteristics of the native cell milieu. Thus, biomaterials engineered in the laboratory not only provide a fertile environment for fostering stem cells but also exhibit properties that elicit favorable crosstalk between the structural components, the cells and the host tissue in a precise physiological manner, enabling balanced matrix generation and degradation for successful tissue regeneration to be achieved. This progress report discusses these advanced biotechnologies for the design, fabrication, and functionalization of biomaterials, highlighting their merits over traditional methodologies for mimicking the structural and mechanical properties of their natural in vivo counterparts.

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Chemotherapeutic Delivery from a Self-Assembling Peptide Nanofiber Hydrogel for the Management of Glioblastoma

et al. 2018

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Growth Factor-Mediated Migration of Bone Marrow Progenitor Cells for Accelerated Scaffold Recruitment

Cited by 24 publications

References 92 publications

Trypsin Pre‐Treatment Combined With Growth Factor Functionalized Self‐Assembling Peptide Hydrogel Improves Cartilage Repair in Rabbit Model

Trypsin Pre‐Treatment Combined With Growth Factor Functionalized Self‐Assembling Peptide Hydrogel Improves Cartilage Repair in Rabbit Model

Advanced Biotechnologies Toward Engineering a Cell Home for Stem Cell Accommodation

Chemotherapeutic Delivery from a Self-Assembling Peptide Nanofiber Hydrogel for the Management of Glioblastoma

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