Growth factors: importance in wound healing and maintenance of transparency of the cornea

Imanishi, Jirô; Kamiyama, Kenji; Iguchi, Ikuo; Kita, Masakazu; Sotozono, Chie; Kinoshita, Shigeru

doi:10.1016/s1350-9462(99)00007-5

Cited by 466 publications

(362 citation statements)

References 39 publications

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“…TGFb is expressed in corneal tissue. [89][90][91][92][93][94][95][96][97] An epithelial defect in the cornea must be rapidly resurfaced to avoid microbial infection and further damage to the underlying stroma. Healing of an epithelial defect is achieved by migration of epithelial cells, followed by an enhancement of cell proliferation for re-establishment of the epithelium stratification.…”

Section: Roles Of Tgfb Siganling In Corneal Wound Healingmentioning

confidence: 99%

TGFβ pathobiology in the eye

Saika

2006

Laboratory Investigation

245

185

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Section: Roles Of Tgfb Siganling In Corneal Wound Healingmentioning

confidence: 99%

TGFβ pathobiology in the eye

Saika

2006

Laboratory Investigation

245

185

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“…They express aldehyde dehydrogenase and keratocan, 1,2 exhibit a dendritic=stellate morphology, interconnect using multiple long cellular processes, 2,3 and help maintain the extracellular matrix (ECM) by synthesizing its components. [4][5][6][7][8] After injury to the corneal stroma, keratocytes transform via a wound healing response into the more active phenotype of fibroblasts, 9 and from there sometimes into myofibroblasts. 10,11 These repair cells migrate to the wound area and secrete a repair ECM (collagen and proteoglycans) 2 that is often fibrotic, fails to restore normal function, and may cause scarring and contraction of the cornea.…”

Section: Introductionmentioning

confidence: 99%

Effect of Serum and Insulin Modulation on the Organization and Morphology of Matrix Synthesized by Bovine Corneal Stromal Cells

Bueno

Saeidi

Melotti

et al. 2009

Tissue Engineering Part A

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The in vitro production of highly organized collagen fibrils by corneal keratocytes in a three-dimensional scaffold-free culture system presents a unique opportunity for the direct observation of organized matrix formation. The objective of this investigation was to develop such a culture system in a glass substrate (for optical accessibility) and to directly examine the effect of reducing serum and=or increasing insulin on the stratification and secretion of aligned matrix by fourth-to fifth-passage bovine corneal stromal keratocytes. Medium concentrations of 0%, 1%, or 10% fetal bovine serum and 0% or 1% insulin-transferrin-selenium were investigated. Highresolution differential interference contrast microscopy, quick-freeze=deep-etch, and conventional transmission electron microscopy were used to monitor the evolution, morphology, and ultrastructure of the cell-matrix constructs. In a medium containing 1% each of serum and insulin-transferrin-selenium, stromal cells stratified and secreted abundant and locally aligned matrix, generating the thickest cell-matrix constructs (allowing handling with forceps). The results of this study have the potential to significantly advance the field of developmental functional engineering of load-bearing tissues by (i) elucidating cues that modulate in vitro cell secretion of organized matrix and (ii) establishing an optically accessible cell culture system for investigating the mechanism of cell secretion of aligned collagen fibrils.

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“…[13][14][15][16] In the eye, all three human isoforms (TGF-b1, TGF-b2 and TGF-b3) have been found although TGF-b2 appears predominant. 17,18 TGF-b has been implicated in the pathogenesis of several ocular scarring diseases such as corneal scarring, 19 proliferative vitreoretinopathy, [20][21][22] cataract and posterior capsular scarring. [23][24][25] It is also involved in the woundhealing response following ocular surgery.…”

Section: Introductionmentioning

confidence: 99%

Novel antisense oligonucleotides targeting TGF-β inhibit in vivo scarring and improve surgical outcome

Cordeiro¹,

Mead²,

Ali

et al. 2003

Gene Ther

158

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The scarring response is an important factor in many diseases throughout the body. In addition, it is a major problem in influencing results of surgery. In the eye, for example, post-operative scarring can determine the outcome of surgery. This is particularly the case in the blinding disease glaucoma, where several anti-scarring regimens are currently used to improve glaucoma surgery results, but are of limited use clinically because of severe complications. We have recently identified transforming growth factor-b (TGF-b) as a target for post-operative anti-scarring therapy in glaucoma, and now report the first study of novel secondgeneration antisense phosphorothioate oligonucleotides against TGF-b in vivo. Single applications of a TGF-b OGN at the time of surgery in two different animal models closely related to the surgical procedure performed in glaucoma patients, significantly reduced post-operative scarring (Po0.05) and improved surgical outcome. Our findings suggest that TGF-b antisense oligonucleotides have potential as a new therapy for reducing post-surgical scarring. Its long-lasting effects after only a single administration at the time of surgery make it particularly attractive clinically. Furthermore, although we have shown this agent to be useful in the eye, it could have widespread applications anywhere in the body where the wound-healing response requires modulation.

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Growth factors: importance in wound healing and maintenance of transparency of the cornea

Cited by 466 publications

References 39 publications

TGFβ pathobiology in the eye

TGFβ pathobiology in the eye

Effect of Serum and Insulin Modulation on the Organization and Morphology of Matrix Synthesized by Bovine Corneal Stromal Cells

Novel antisense oligonucleotides targeting TGF-β inhibit in vivo scarring and improve surgical outcome

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