Growth fraction in centrocytic and follicular center cell lymphomas: Assessment in paraffin sections with a proliferating cell nuclear antigen antibody and morphometric correlates

Swerdlow, Steven H.; Westermann, Cindy D.; Pelstring, Richard J.; Saboorian, M. Hossein; Williams, Michael E.

doi:10.1016/0046-8177(93)90167-f

Cited by 11 publications

(3 citation statements)

References 36 publications

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“…Our semi-quantitative method of determining PI by estimating Ki-67-positive cells is a practical method applicable to daily practice. [18][19][20]25,30,31 In our FL cases with concurrent DLBCL, the PI in the DLBCL areas was even higher than in FL grade 3 (Table 1), suggesting biologic differences between FL grade 3 and transformed FL. This method avoids the pitfall of image analysis in which the larger cells (centroblasts), by occupying more area, give proportionally more signals than the smaller cells (centrocytes).…”

Section: Discussionmentioning

confidence: 99%

Low Histologic Grade Follicular Lymphoma With High Proliferation Index

Wang¹,

Wang²,

Hochberg³

et al. 2005

The American Journal of Surgical Pathology

104

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Histologic grading has been used as a guide for clinical management in follicular lymphoma (FL). Proliferation index (PI) of FL generally correlates with tumor grade; however, in cases of discordance, it is not clear whether histologic grade or PI correlates with clinical aggressiveness. To objectively evaluate these cases, we determined PI by Ki-67 immunostaining in 142 cases of FL (48 grade 1, 71 grade 2, and 23 grade 3). A total of 24 cases FL with low histologic grade but high PI (LG-HPI) were identified, a frequency of 18%. On histologic examination, LG-HPI FL often exhibited blastoid features. Patients with LG-HPI FL had inferior disease-specific survival but a higher 5-year disease-free rate than low-grade FL with concordantly low PI (LG-LPI). However, transformation to diffuse large B-cell lymphoma was uncommon in LG-HPI cases (1 of 19; 5%) as compared with LG-LPI cases (27 of 74; 36%). In conclusion, LG-HPI FL appears to be a subgroup of FL with clinical behavior more akin to grade 3 FL. We propose that these LG-HPI FL cases should be classified separately from cases of low histologic grade FL with concordantly low PI.

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Section: Discussionmentioning

confidence: 99%

Low Histologic Grade Follicular Lymphoma With High Proliferation Index

Wang¹,

Wang²,

Hochberg³

et al. 2005

The American Journal of Surgical Pathology

104

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show abstract

“…Other methods include determination of mitotic index (6) and immunohistocheniical staining for proliferation associated markers, e.g. Ki-67 (7) or PCNA (8)(9)(10)(11). One advantage with the immunohistochemical approach is that antigen of interest can be selectively evaluated in tumour cell population.…”

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confidence: 99%

Flow Cytometric Evaluation of Proliferating Cell Nuclear Antigen Expression in Human Hematopoietic Malignancies

et al. 1997

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“…The proliferative tumour fraction was assessed by manual count of mitoses and by immunostaining with an antibody directed against proliferative cell nuclear antigen (PCNA). Follicular and diffuse areas of tumour growth were measured separately, with an average taken of the count of three fields at 1000× magnification and normalized to the number of CD20+ tumour cells 23. Mitotic figures (MFs) were counted in ten high‐power fields (HPFs) under 400× magnification.…”

Section: Methodsmentioning

confidence: 99%