Growth, gonadal development, and fish fillet quality in diploid and triploid turbot, Scophthalmus maximus, originated from the Black Sea

Aydın, İlhan; Terzi, Yahya; Polat, Hamza; Küçük, Ercan; Öztürk, Rafet Çağrı

doi:10.1016/j.aquaculture.2022.738558

Cited by 5 publications

(1 citation statement)

References 27 publications

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“…Most of triploid fish have a growth advantage over diploid fish (Okomoda et al 2020 ; Tiwary et al 2004 ). However, the growth advantage of triploids is not always apparent (Aydın et al 2022 ; do Nascimento et al 2017 ; Mol et al 1994 ), and some studies have found that triploid fish have greater mortality than diploid fish (Cotter et al 2000 ; Koenig et al 2011 ). Meanwhile, there are also numerous studies of gonadal development in triploid fish.…”

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confidence: 99%

Production of sterile mono-sex triploid yellow drum (Nibea albiflora): genotypic females and sex-reversed phenotypic males with emphasis on utilization as surrogate broodstock

Yang,

Lu,

Chen

et al. 2023

Fish Physiol Biochem

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Production of sterile mono-sex fish is of great significance for sustainable aquaculture as well as germ cell transplantation. In this study, we aimed to produce mono-sex triploid yellow drum, including genotypic females (XXX female) and sex-reversed phenotypic males (XXX male). Firstly, the mono-female triploids were produced through cold-shock treatment on eggs fertilized with sperm from neo-males. Then, the mono-male triploids were produced by the sex reversal of mono-female triploids with oral administration of letrozole (LZ). We comparatively investigated the growth and gonadal development in the mono-sex triploids. The results showed that the triploids displayed similar growth performance to their diploids throughout their first year, but had impaired gonadosomatic index and gametogenesis. No mature gametes were produced in the triploids during their first spawning season. Meanwhile, we analyzed the process of gametogenesis in the both sex of triploids. Ultrastructure of gametogenesis showed that the germ cells arrested at abnormal metaphase 1 in females, while males had irregular meiotic divisions, variable-sized spermatid and degenerated cells. The expression levels of meiosis-related genes (i.e., sycp3 and rec8) confirmed the abnormal meiosis in the triploids. Furthermore, the gonadal development was also determined by the expression patterns of vasa, dmrt1 and cyp19a1a. Abnormal expression of vasa mRNA and protein were detected in triploids. High cyp19a1a expression levels suggested the sex steroid hormones production might be at least partially functional in triploid females. In addition, high dmrt1 expression levels confirmed the masculinization and testicular development of sex-reversed triploid males by LZ. Our findings provide an efficient protocol to produce sterile mono-sex triploid yellow drum and provide new insights into the mechanism of gonadal sterility of triploid fish.

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