Growth hormone-like substance in the rotifer Brachionus plicatilis

Gallardo, Wenresti G.; Hagiwara, Atsushi; Hara, Kenji; Soyano, Kiyoshi

doi:10.1111/j.1444-2906.2006.01218.x

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…In this regard, it is interesting to raise the antibody against the isolated protein in order to obtain other possible proteins having similar functions from CM. Gallardo et al (2006) isolated a growth hormonelike protein with a molecular weight of 25000 from the CE from the rotifer B. plicatilis NH3 strain. Furthermore, Snell et al (2006) isolated the steroidogenesis-inducing protein (SIP) with a molecular weight of 39000 from CM of the rotifer B. plicatilis RUS strain.…”

Section: Discussionmentioning

confidence: 99%

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

et al. 2011

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We confirmed the existence of growthpromoting substances in the conditioned media (CM) from the rotifer Brachionus plicatilis at an early exponential growth phase and isolated a novel protein with a growth-promoting activity from the crude extract (CE) of rotifer cells. CM was prepared from the culture media where rotifers had been cultured at an early exponential growth phase and filtered through a 0.22-lm filter membrane. The growth-promoting activity was determined using rotifers in CM for 5 days. As a result, the increase of rotifers added with CM was significantly higher than that of the control in artificial seawater (P \ 0.001). Moreover, the growthpromoting activity of CM was dose-dependent and inactivated by heat treatment at 80°C for 60 min. Meanwhile, CM filtered through a \10 kDa ultrafiltration membrane showed a low activity, whereas proteinase K treatment resulted in a complete inactivation. These results suggest that the rotifer secrets growth-promoting proteins into CM. CE also contained a protein with the activity and properties similar to those found in CM. Then, CE was subjected to purification of a growth-promoting protein for convenience using various types of chromatography after fractionation with 30-80% saturated ammonium sulfate. Subsequently, a protein with an approximate molecular weight of 25000 was isolated, and its N-terminal amino acid sequence was determined to be PAVVDFTAVWFGPLQMIKP. An orthologue was found in the EST database of B. plicatilis, the full sequence of which showed about 50% identity to the corresponding regions of thioredoxins from other organisms.

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Section: Discussionmentioning

confidence: 99%

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

et al. 2011

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“…Several studies about the effect of hormones on rotifers have been focused on the population and sexual reproduction parameters in an ecotoxicology paradigm. A few studies have suggested that vertebrate and invertebrate hormones, such as growth hormone, human chorionic gonadotropin, juvenile hormone and 17β-estradiol, affect asexual population growth and mictic reproduction in the rotifers Brachionus plicatilis and Brachionus calyciflorus (GALLARDO et al, 1997;PRESTON and SNELL, 2001;RADIX et al, 2002;GALLARDO et al, 2006). A receptor similar to the vertebrate progesterone receptor was discovered in the rotifer Brachionus manjavacas (SNELL and DESROSIERS, 2008;SMITH et al, 2010;STOUT et al, 2010).…”

Section: Introductionmentioning

confidence: 98%

Effects of Progesterone, Testosterone, and Estrogen on Sexual Reproduction of the RotiferBrachionus calyciflorus

Yang

Snell

2010

International Review of Hydrobiology

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It is known that some vertebrate reproductive hormones have effects on rotifer reproduction, but little is known about their effects in combination. This motivated us to examine the effects of waterborne progesterone (P), testosterone (T), and estradiol (E) on reproduction of the rotifer Brachionus calyciflorus, comparing exposures to single hormones and mixtures with a total concentration of 1000 μg/L. Asexual population growth (r) was high in all treatments, ranging from 1.28 to 1.43 d -1 (P-E), and from 0.99 to 1.22 d -1 (P-T). These results indicate that E, P, or T alone, or in combinations of P-E and P-T totaling 1000 μg/L, and the 3-way combination P-E-T at 1200 μg/L do not reduce the asexual reproduction of B. calyciflorus. However, significant effects were detected on sexual reproduction with exposure to these hormone concentrations. Exposure to P-T and P-E at 1000 μg/L decreased resting egg production significantly. Analysis of mating indicated that fertilization was 5.3 times higher in the control than in treatments where females were exposed to 500 μg/L each P-T. A similar result was observed when both females and males were exposed to 500 μg/L each of P-T. When males alone were treated with 500 μg/L each of P-T, there was no significant difference in male fertility compared to the control. These results suggest that B. calyciflorus uses oxidized sterols similar to P and T to regulate sexual reproduction and waterborne exposure of females to these compounds interferes with fertilization and resting egg production.

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“…Ominato et al identified an hGH-like protein in the pituitary of Petromyzon marinus in 2002 [8] . Gallardo et al found an hGH-like protein in rotifer using antibodies to the growth hormone of salmon, and demonstrated its function in promotion of rotifer reproduction [9] . Phares’s research group conducted a study on Spirometra mansonoides and found that the parasite secreted a protein that can bind to the hGH receptor [10] .…”

Section: Introductionmentioning

confidence: 99%

Purification and Functional Characterization of a Protein: Bombyx mori Human Growth Hormone Like Protein in Silkworm Pupa

Shu

Nie

et al. 2014

PLoS ONE

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Human growth hormone (hGH) is a peptide hormone secreted by eosinophils of the human anterior pituitary, and a regulatory factor for a variety of metabolic pathways. A 30-kD protein from the pupa stage of silkworm was detected by Western blotting and confirmed by immunoprecipitation based on its ability to bind to anti-hGH antibody. This protein, named BmhGH-like protein, was purified from fresh silkworm pupas through low-temperature homogenization, filtration, and centrifugation to remove large impurity particles. The supernatants were precipitated, resuspended, and passed through a molecular sieve. Further purification by affinity chromatography and two-dimensional electrophoresis resulted in pure protein for analysis by MS MALDI-TOF-MS analysis. An alignment with predicted proteins indicated that BmhGH-like protein consisted of two lipoproteins, which we named hGH-L1 and hGH-L2. These proteins belong to the β-trefoil superfamily, with β domains similar to the spatial structure of hGH. Assays with K562 cells demonstrated that these proteins could promote cell division in vitro. To further validate the growth-promoting effects, hGH-L2 was cloned from pupa cDNA to create recombinant silkworm baculovirus vBmNPV-hGH-L2, which was used to infect silkworm BmN cells at low titer. Flow cytometric analysis demonstrated that the protein shortened the G0/G1 phase of the cells, and enabled the cells to rapidly traverse the G1/S phase transition point to enter S phase and promote cell division. Discovery of hGH-like protein in silkworm will once again arouse people’s interest in the potential medicinal value of silkworm and establish the basis for the development of new hormone drugs.

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Growth hormone-like substance in the rotifer Brachionus plicatilis

Cited by 7 publications

References 27 publications

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

Effects of Progesterone, Testosterone, and Estrogen on Sexual Reproduction of the RotiferBrachionus calyciflorus

Purification and Functional Characterization of a Protein: Bombyx mori Human Growth Hormone Like Protein in Silkworm Pupa

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