Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)

Zhong, Chengrong; Song, Yanlong; Wang, Yaping; Li, Yongming; Liao, Lanjie; Xie, Shouqi; Zhang, Zhu; Hu, Wei

doi:10.1016/j.aquaculture.2012.04.019

Cited by 37 publications

(35 citation statements)

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“…The fast-growing generations of homozygous and hemizygous GH-transgenic common carp were obtained as described previously [6]. All procedures were conducted in accordance with the Guiding Principles for the Care and Use of Laboratory Animals and were approved by Institute of Hydrobiology, Chinese Academy of Sciences.…”

Section: Methodsmentioning

confidence: 99%

“…The templates were diluted to 10 8 , 10 7 , 10 6 , 10 5 , 10 4 , and 10 3 copies/lL. Real-time PCR was performed as described previously [6], and standard curves were constructed.…”

Section: Real-time Pcrmentioning

confidence: 99%

“…Since the first batch of transgenic fish were produced, there have been over 35 fish species that have been manipulated using transgenic techniques, including commercial aquaculture fishes such as common carp (Cyprinus carpio), grass carp (Ctenopharyngodon idellus), Nile tilapia (Oreochromis niloticus), channel catfish (Ietalurus punetaus), rainbow trout (Oncorhynchus mykiss), coho salmon (Oncorhynchus kisutch), Atlantic salmon (Salmo salar), and mud loach (Misgurnus mizolepis) [6], as well as model fishes such as zebrafish (Danio rerio) and medaka (Oryzias latipes). It is common that the introduced traits are unstable or weakly expressed in the transgenic groups, although some individuals do present enhanced characteristics [7].…”

Section: Introductionmentioning

confidence: 99%

“…So far, the GH gene has been transferred successfully into several salmons, several tilapias, channel catfish, rohu (Labeo rohita), mud loach, and common carp [6,[23][24][25][26][27][28][29][30]. However, studies of integration characteristics, such as exogenous copy number, integration sites and structure of expression elements, have been conducted only in coho salmon and Atlantic salmon [11,31,32].…”

Section: Introductionmentioning

confidence: 99%

“…1a), was microinjected into fertilized eggs of common carp. After years of test crossing and self-crossing, a GH-gene homozygous transgenic common carp line has been established [6]. This transgenic line expressed high-level GH in extensive tissues persistently and showed fastgrowth performance [33], which suggests successful exogenous gene transfer.…”

Section: Introductionmentioning

confidence: 99%

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The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

et al. 2015

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Section: Methodsmentioning

confidence: 99%

“…The templates were diluted to 10 8 , 10 7 , 10 6 , 10 5 , 10 4 , and 10 3 copies/lL. Real-time PCR was performed as described previously [6], and standard curves were constructed.…”

Section: Real-time Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

et al. 2015

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Ecological implications of genetically modified fishes in freshwater fisheries, with a focus on salmonids

Sundström

Devlin

2015

Freshwater Fisheries Ecology

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Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone Development in Common Carp

et al. 2013

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Natural selection favors submaximal rather than maximal growth. The importance of body size and growth rate in ecological interactions are widely recognized, and both of them are frequently used as surrogates for fitness (Biro et al., 2006). Empirical evidence for increased advantages (e.g., survival rate, fecundity, and competitive ability) in fast growing individuals and/or populations was well documented (e.g., Roff, '92; Stearns, '92;Urban, 2007). It is therefore difficult to explain the maintenance of genetic variation in growth rates (Mangel and Stamps, 2001). One explanation is that there is a trade-off related to the fast growth. This cost may pose a selective pressure that ABSTRACTLimited information is available on effects of growth hormone transgene and nutrition on growth and development of aquatic animals. Here, we present a study to test these effects with growthenhanced transgenic common carp under two nutritional conditions or feeding rations (i.e., 5% and 10% of fish body weight per day). Compared with the nontransgenic fish, the growth rates of the transgenic fish increased significantly in both feeding rations. The shape of the pharyngeal bone was similar among treatments, but the transgenic fish had relatively smaller and lighter pharyngeal bone compared with the nontransgenic fish. Calcium content of the pharyngeal bone of the transgenic fish was significantly lower than that of the nontransgenic fish. Feeding ration also affected growth rate but less of an effect on bone development. By manipulating intrinsic growth and controlling for both environment (e.g., feeding ration) and genetic background or genotype (e.g., transgenic or not), this study provides empirical evidence that the genotype has a stronger effect than the environment on pharyngeal bone development. The pharyngeal bone strength could be reduced by decreased calcium content and calcification in the transgenic carp. J. Exp. Zool.319A: 451-460, 2013.

show abstract

Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)

Cited by 37 publications

References 50 publications

The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

Ecological implications of genetically modified fishes in freshwater fisheries, with a focus on salmonids

Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone Development in Common Carp

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