Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells

Orosz, David; Resnick, Martin I.; Wehland, Jürgen; Douglas, Janice G.; Edwards, John; Jacobberger, James W.; Hopfer, Ulrich; Woost, Philip G.; Kolb, Robert; Finesilver, Margaret; Jin, Wenwu; Frisa, Phyllis; Choo, Cheow-Keong; Yau, Chung-Fai; Chan, Kwok–Wah

doi:10.1290/0308062

Cited by 14 publications

(18 citation statements)

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“…26,27 Inverse genomic PCR 29 analysis of DNA from this clone confirmed the insertion of the gene-trapping retroviral vector in intron 1 of the Arl3 gene on chromosome 19, downstream of the initiation codon in exon 1 ( Figure 1A). OST 263303 cells were used to generate mice heterozygous for the Arl3 mutation using standard methods, 28 and genotypes were confirmed by multiplex PCR on genomic DNA ( Figure 1B).…”

Section: Mouse Es Cellsmentioning

confidence: 66%

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ADP-Ribosylation Factor-Like 3 Is Involved in Kidney and Photoreceptor Development

Schrick

Vogel

Abuin

et al. 2006

The American Journal of Pathology

140

114

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ADP-ribosylation factor-like 3 (Arl3) is a member of a small subfamily of G-proteins involved in membraneassociated vesicular and intracellular trafficking processes. Genetic studies in Leishmania have shown that the Arl3 homolog is essential for flagellum biogenesis. Mutations in a related human family member, Arl6, result in Bardet-Biedl syndrome in humans, which is characterized by genital, renal, and retinal abnormalities, obesity, and learning deficits. As part of our largescale phenotypic screen, mice deficient for the Arl3 gene were generated and analyzed. Arl3 (؊/؊) mice were born at a sub-Mendelian ratio, were small and sickly, and had markedly swollen abdomens. These mutants failed to thrive, and all died by 3 weeks of age. The (؊/؊) mice exhibited abnormal development of renal, hepatic, and pancreatic epithelial tubule structures, which is characteristic of the renal-hepatic-pancreatic dysplasia found in autosomal recessive polycystic kidney disease. Absence of Arl3 was associated with abnormal epithelial cell proliferation and cyst formation. Moreover, mice lacking Arl3 exhibited photoreceptor degeneration as early as postnatal day 14. These results are the first to implicate Arl3 in a ciliary disease affecting the kidney, biliary tract, pancreas, and retina.

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Section: Mouse Es Cellsmentioning

confidence: 66%

“…28 The precise genomic insertion site of the retroviral gene-trapping vector in the Arl3 gene was determined by inverse genomic polymerase chain reaction (PCR). 29 …”

Section: Generation Of Arl3 Mutant Es Cells and Micementioning

confidence: 99%

ADP-Ribosylation Factor-Like 3 Is Involved in Kidney and Photoreceptor Development

Schrick

Vogel

Abuin

et al. 2006

The American Journal of Pathology

140

114

View full text Add to dashboard Cite

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“…The particular feature of the temperature-sensitive tsA58 mutant has been used to analyze the changes in phenotype and gene expression in renal tubule cells in growing state (33°C) and after growth arrest (39.5°C; see below). Using this strategy, three human cell lines have been derived from primary cultures of the PCT S1 microdissected segments and cocultured with irradiated 3T3 fibroblasts that had been infected with a retroviral construct encoding the wild-type SV40 Tag or the mutant SV40tsA58 (ts) [87]. Human PCT cells have also been isolated from the urine of patients with cystinosis and exposed to an immortalizing recombinant retrovirus construct carrying the temperature-sensitive SV40TtsA58 and U19 mutations conferring temperature sensitivity and enhanced transfection efficiency, respectively, in order to establish suitable cell system for in vitro analyses of tubule epithelial cells and lysosomal abnormalities in cystinosis [103].…”

Section: Immortalized Human Proximal and Collecting Duct Cellsmentioning

confidence: 99%

Cell models for studying renal physiology

Bens

Vandewalle

2008

Pflugers Arch - Eur J Physiol

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The development over the past 20 years of a variety of cultured renal tubule cell lines derived from different parts of the renal tubule has provided invaluable powerful cell systems for in vitro analyses of the various tubule segment-specific biochemical functions and ion transport processes. Immortalized cell lines have been established using different hybrid gene constructs, most of them carrying the immortalizing simian virus 40 large T antigen (Tag) gene. The development of transgenic mice carrying unregulated Tag, and of others in which the expression of Tag remains controlled, has made it possible to establish permanent cell lines derived from microdissected or immunoselected renal proximal, distal, and collecting duct tubules. This review summarizes the different strategies of cellular immortalization used and the most frequently used human, rabbit, rat, and mouse tubule cell lines. This review provides an overview of the use of immortalized mouse tubule cell lines for in vitro analyses of various tubule cell-specific functions and the regulation of ion transporters and membranous channels. The advantages of using primary cultures of isolated tubules dissected from physiopathological models of transgenic mice are also discussed.

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“…SV40 large T-antigen has been used to immortalize other cell types such as human proximal tubule epithelial cells. These cell lines retain their differentiation capacity as seen by their ability to form electrically resistant monolayers with apical microvilli, tight junctional complexes and expression of numerous markers for proximal tubule cells [75]. In contrast, preadipocytes show aberrant differentiation after immortalization with SV40 large T-antigen [76].…”

Section: Ectopic Immortalization Protocolsmentioning

confidence: 99%

Immortalization protocols used in cell culture models of human breast morphogenesis

Gudjonsson

Villadsen²,

Rønnov‐Jessen³

et al. 2004

CMLS, Cell. Mol. Life Sci.

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Defining the key players in normal breast differentiation is instrumental to understanding how morphogenesis becomes defective during breast cancer progression. During the past 2 decades much effort has been devoted to the development of technologies for purification and expansion of primary human breast cells in culture and optimizing a relevant microenvironment, which may help to define the niche that regulates breast differentiation and morphogenesis. In contrast to the general property of cancer, normal human cells have a finite lifespan. After a defined number of population doublings, normal cells enter an irreversible proliferation-arrested state referred to as replicative senescence. To overcome this obstacle for continuous long-term studies, replicative senescence can be bypassed by treatment of cells with chemical agents such as benzopyrene, by radiation or by transfection with viral oncogenes or the gene for human telomerase (human telomerase reverse transcriptase, hTERT). A drawback of some of these protocols is a concurrent introduction of chromosomal changes, which sometimes leads to a transformed phenotype and selection of a subpopulation, which may not be representative of the tissue of origin. In recent years, we have sought to establish immortalized primary breast cells, which retain crucial characteristics of their original in situ tissue pattern. This review discusses various approaches to immortalization of breast-derived epithelial and stromal cells and the application of such cell lines for studies on human breast morphogenesis.

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Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells

Cited by 14 publications

References 0 publications

ADP-Ribosylation Factor-Like 3 Is Involved in Kidney and Photoreceptor Development

ADP-Ribosylation Factor-Like 3 Is Involved in Kidney and Photoreceptor Development

Cell models for studying renal physiology

Immortalization protocols used in cell culture models of human breast morphogenesis

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