Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria

Abstract

The growth-inhibiting activities of the essential oils extracted from Platycladus orientalis leaves and stems were examined against intestinal bacteria. The leaf oil exhibited a strong response against Clostridium difficile and C. perfringens at 5.0 mg/disc, but the stem oil showed no inhibitory activity against intestinal bacteria. The oils from P. orientalis leaves and stems were analyzed by GCMass spectroscopy. The main constituents of the essential oil in P. orientalis leaves: α-pinene (18.5%), cedrene (13.6%), α-cedrol (11.3%), β-caryophyllene (10.2%), 3-carene (8.2%), and α-terpinyl acetate (5.1%), and in the stems: α-pinene (17.7%), 3-carene (16.3%), β-caryophyllene (13.4%), β-phellandrene (9.5%), β-selinene (6.5%), and α-cedrol (6.1%). The cedrene isomers showed significant (33.1-34.7 mm) inhibitory effect against C. difficile and C. perfringens and moderate inhibitory effect against Escherichia coli at 2.0 mg/disc. The oil of P. orientalis leaves, in which cedrene isomers are the major component, could be used as a selective inhibitor against harmful bacteria.

Physicochemical Characterization of a Polysaccharide Fraction from Platycladus orientalis (L.) Franco and Its Macrophage Immunomodulatory and Anti-Hepatitis B Virus Activities

“…3 Previous studies demonstrated that P. orientalis has a high medicinal value due to its antimicrobial, anti-inflammatory, antihyperlipidemic, and neuroprotective biological activities. 4−7 Studies into P. orientalis have been mostly focused on its flavonoid components for antioxidant activity 8 and antihyperuricemic effect; 9 essential oils for antimicrobial activity; 4 and diterpenes for antifibrotic activity 10 and anti-inflammatory activity. 11 Besides, polysaccharides are considered as one of the major bioactive compounds of P. orientalis; however, little information about their structure and biological activities is available.…”

“…The leaves and seeds of Platycladus orientalis (L.) Franco are used as functional foods with a long history of treating for inflammation, gout, asthma, etc. , It is also safe to be used as functional food materials approved by China Food and Drug Administration (CFDA) . Previous studies demonstrated that P. orientalis has a high medicinal value due to its antimicrobial, anti-inflammatory, antihyperlipidemic, and neuroprotective biological activities. − …”

Analysis of Floral Scent Component of Three Iris Species at Different Stages

“…O-isopropylbenzene is primarily employed in creating imitation products and fixed fragrances [38]. β-Caryophyllene is mainly used for preparing fine imitation products and fragrance fixatives [39].…”

Actinomycetes as Producers of Biologically Active Terpenoids: Current Trends and Patents

“…KO-3988 [234], S. griseus CB00830 [235], and Streptomyces sp. SN194 [152] synthesized novel oxaloterpins A-E (347)(348)(349)(350)(351). Two new Cl-containing diterpenoids chloroxaloterpins A (352) and B (353) containing unique groups [(2-chlorophenyl)amino]carbonyl and 2-[(2-chlorophenyl)amino]-2-oxo-acetyl, respectively, were identified among the metabolites of Streptomyces sp.…”

“…KO-3988 [234], S. griseus CB00830 [235], and Streptomyces sp. SN194 [152] synthesized novel oxaloterpins A-E (347)(348)(349)(350)(351). Two new Cl-containing diterpenoids chloroxaloterpins A (352) [232], catalyzed the formation of the N,S-containing labdane diterpenoid cyslabdan A (341) and its 18-hydroxy-(cyslabdan B, 342) and 1 -methoxy-(cyslabdan C, 343) derivatives [233].…”