Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8

Lee, Nam Keun; Yeo, In-Cheol; Park, Joung Whan; Hahm, Young Tae

doi:10.1007/s12010-011-9246-7

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…31, 51, 52 Furthermore, B. anthracis GroEL was found to be phosphorylated in biofilm-forming cells, indicating the necessity of the active protein for biofilm formation. Under these conditions, GroEL might promote the repair of damaged proteins or facilitate the folding of other overexpressed proteins.…”

Section: Discussionmentioning

confidence: 97%

Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis

Arora

Sajid

Virmani

et al. 2017

npj Biofilms Microbiomes

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PrkC is a conserved Ser/Thr protein kinase encoded in Bacillus anthracis genome. PrkC is shown to be important for B. anthracis pathogenesis, but little is known about its other functions and phosphorylated substrates. Systemic analyses indicate the compelling role of PrkC in phosphorylating multiple substrates, including the essential chaperone GroEL. Through mass spectrometry, we identified that PrkC phosphorylates GroEL on six threonine residues that are distributed in three canonical regions. Phosphorylation facilitates the oligomerization of GroEL to the physiologically active tetradecameric state and increases its affinity toward the co-chaperone GroES. Deletion of prkC in B. anthracis abrogates its ability to form biofilm. Overexpression of native GroEL recovers the biofilm-forming ability of prkC deletion strain. Similar overexpression of GroEL phosphorylation site mutants (Thr to Ala) does not augment biofilm formation. Further analyses indicate the phosphorylation of GroEL in diverse bacterial species. Thus, our results suggest that PrkC regulates biofilm formation by modulating the GroEL activity in a phosphorylation-dependent manner. The study deciphers the molecular signaling events that are important for biofilm formation in B. anthracis.

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Section: Discussionmentioning

confidence: 97%

Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis

Arora

Sajid

Virmani

et al. 2017

npj Biofilms Microbiomes

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“…AMP IC-1 also has narrow antagonistic spectrum to the B. cereus group (data not shown). We previously showed that BSAP-254 affects the release of DNA and K + and induces the expression of stress protein GroEL in B. cereus [32]. Antimicrobial peptides such as gramicidin S and polymyxin B are known to kill bacterial cells by membrane perforation, degradation of cell walls, and finally micellization [33].…”

Section: Discussionmentioning

confidence: 99%

Interspecies Interaction of Signal Peptide PapR Secreted by Bacillus cereus and Its Effect on Production of Antimicrobial Peptide

Yeo

Lee

Cha

et al. 2011

Appl Biochem Biotechnol

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This study was carried out to investigate the interspecies interaction of PapR peptide secreted by Bacillus cereus on production of BSAP-254, an antimicrobial peptide produced by Bacillus subtilis SC-8 isolated from the Korean fermented soybean paste and exhibited narrow antagonistic activity against the B. cereus group, but not against other foodborne pathogens. PapR is a signal peptide that activates PlcR, which is a pleiotropic regulator controlling the expression of various virulence factors in B. cereus. When B. subtilis SC-8 was co-cultured with B. cereus, it completely inhibited the growth of B. cereus within 12 h, and the rate of BSAP-254 production was increased 34.2% at 12 h. Furthermore, 5 μM of synthetic PapR peptide added to the culture of B. subtilis SC-8 increased the rate of BSAP-254 production up to 59.7%. The growth of B. subtilis SC-8, however, was not significantly different with or without the addition of PapR. When B. cereus papR mutant was co-cultured with B. subtilis SC-8, the growth of the mutant was not inhibited and the rate of BSAP-254 production was decreased by 45%.

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“…Bacillus subtilis SC-8 is a gram-positive bacterium that has been isolated from fermented soy. The microorganism inhibits the growth of Bacillus cereus species as Bacillus anthracis, Bacillus mycoides, Bacillus pseudomycoides, Bacillus thuringiensis, and Bacillus weihenstephanensis [60,61]. Genes were also found on chromosome SC-8 of Bacillus subtilis (BSSC8_21740 to BSSC8_21920), these genes can code new antimicrobial peptides against B. cereus.…”

Section: Bacillus Subtilis Sc-8mentioning

confidence: 99%

Peptides and Microorganisms Isolated from Soybean Sources with Antimicrobial Activity

Mora‐Escobedo¹,

Robles-Ramírez²,

Román‐Gutiérrez³

et al. 2019

Soybean - Biomass, Yield and Productivity

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Soybean has a high biological value because it is a potential nutraceutical that benefits human health. Isolated peptides of soybean have been associated with activities such as anticancer, antioxidants, antiobesity, antithrombotics, hypocholesterolemic, antidiabetic, immunomodulatory and antimicrobial, and this last activity is also obtained from microorganisms isolated from soybean subjected to processes such as fermentation, which can act directly against pathogens that are resistant to antibiotics or participate in the synthesis of new peptides with antimicrobial activity, so they could be used as preservatives in food as an alternative in the prevention of diseases. Strains of Bacillus subtilis isolated from soybean are mainly those that have the ability to inhibit the growth and proliferation of pathogens; some fungi such as Rhizopus microspores and Aspergillus oryzae have also had an inhibitory effect. This chapter describes the potential of microorganisms and peptides obtained from different sources of soybean against pathogenic microorganisms responsible for foodborne diseases.

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Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8

Cited by 6 publications

References 25 publications

Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis

Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis

Interspecies Interaction of Signal Peptide PapR Secreted by Bacillus cereus and Its Effect on Production of Antimicrobial Peptide

Peptides and Microorganisms Isolated from Soybean Sources with Antimicrobial Activity

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