Growth inhibition and possible mechanism of oleamide against the toxin-producing cyanobacterium Microcystis aeruginosa NIES-843

Shao, Jihai; He, Yaxian; Fan, L.T.; Zhang, Huiling; Luo, Si; Gu, Ji‐Dong

doi:10.1007/s10646-015-1582-x

Cited by 18 publications

(8 citation statements)

References 32 publications

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“…There was no typical J, I, and P phases in the treatment of pH 4.0, meaning that PS II was severely damaged when the pH fell to 4.0. The PI abs is a general index reflecting the performance of PSII [ 29 ]. As indicated in Figure 3 , PI abs was severely inhibited by the acidification of paddy floodwater.…”

Section: Discussionmentioning

confidence: 99%

Physiological Responses of a Diazotrophic Cyanobacterium to Acidification of Paddy Floodwater: N2 Fixation, Photosynthesis, and Oxidative–Antioxidative Characteristics

Yan

Xiao

Li³

et al. 2022

IJERPH

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Long-term of excessive fertilization using nitrogen (N) chemical fertilizer caused the acidification of paddy soils. Presently, the impacts of soil acidification on physiological characteristics of diazotrophic cyanobacteria remain unknown. In order to elucidate this issue, the effects of paddy floodwater acidification on activities of respiration, photosynthetic oxygen evolution, and N2 fixation of a paddy diazotrophic cyanobacterium Aliinostoc sp. YYLX235 were investigated in this study. In addition, the origination and quenching of intracellular reactive oxygen species (ROS) were analyzed. The acidification of paddy floodwater decreased intracellular pH and interfered in energy flux from light-harvesting chlorophyll antenna to the reaction center of photosystem II (PS II). Activities of respiration, photosynthetic oxygen evolution, and N2 fixation were decreased by the acidification of paddy floodwater. Accompanied with an increase in ROS, the level of antioxidative system increased. Superoxide dismutase (SOD) and catalase (CAT) were the main enzymatic ROS scavengers in the cells of YYLX235; reduced glutathione (GSH) was the main non-enzymatic antioxidant. Antioxidants and oxidants in the cells of YYLX235 lost balance when the pH of paddy floodwater fell to 5.0 and 4.0, and lipid oxidative damage happened. The results presented in this study suggest that the acidification of paddy soil severely interfered in the photosynthesis of diazotrophic cyanobacteria and induced the production of ROS, which in turn resulted in oxidative damage on diazotrophic cyanobacteria and a decrease in cell vitality.

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Section: Discussionmentioning

confidence: 99%

Physiological Responses of a Diazotrophic Cyanobacterium to Acidification of Paddy Floodwater: N2 Fixation, Photosynthesis, and Oxidative–Antioxidative Characteristics

Yan

Xiao

Li³

et al. 2022

IJERPH

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“…As a prokaryote, M. aeruginosa only contains a thylakoid cell membrane (Shao et al 2016),which contains a variety of enzymes required for physiological and biochemical reactions (Lee et al 2018). The lack of a cell wall and the delicate cell membranes renders the outermost layer of the cell vulnerable to allelochemicals released by Spirogyra, and it is likely that the inhibition effect on the alga was due to a loss of cell membrane integrity that led to a disruption of enzymatic pathways and metabolic activity in the algae.…”

Section: Discussionmentioning

confidence: 99%

Combined allelopathic effects of Spirogyra (Zygnematales: Zygnemataceae) and Ceratophyllum demersum (Ceratophyllales: Ceratophyllaceae) on the growth of Microcystis aeruginosa (Chroocolales: Microcystaceae)

Hui

Huang

et al. 2019

Biologia

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Allelopathic inhibition activity of Spirogyra and Ceratophyllum demersum (C. demersum) on Microcystis aeruginosa were compared using a series of experiments. Culture solution of Spirogyra was found to inhibit growth of M. aeruginosa resulting in the destruction of chlorophyll-a and increased MDA content of the algae. The inhibitory effect was increased with greater concentrations of macrophyte culture solution and with continuous addition of the macrophytes. Filter size also had an effect in the inhibition of M. aeruginosa growth. Poor algal growth may have resulted from metabolic secretions from Spirogyra and associated epiphytic bacteria. Inhibition of algal growth and growth of the macrophytes and tolerance of Spirogyra to eutrophic conditions were further improved by synergistic effects of co-existing Spirogyra and C. demersum. Maximum inhibition effects and macrophyte biomass were recorded when the ratio of Spirogyra to C. demersum was 6:4.

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“…The qPCR reaction was prepared with final volumes of 10 μL containing 5 μL of Master Mix (SYBR Green, CWBIO, China), 0.2 μL of each primer (10 μmol L −1 ), and 0.4 μL cDNA and 4 μL of sterile water. The qPCR conditions were set as follows: 95 °C for 10 min; 40 cycles at 95 °C for 15 s, 59 °C for 30 s, and 72 °C for 30 s. The relative transcription level of the mcyB gene was calculated using 2 −ΔΔCt where ΔΔCt = (Ct target gene − Ct 16S rrn ) stress − (Ct target gene − Ct 16Srrn ) control [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Inhibition Effect of Ionic Liquid [Hmim]Cl on Microcystis Growth and Toxin Production

Liu

Zhang

Sultan

et al. 2022

IJERPH

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Ionic liquids (ILs) are known as “green solvents” and widely used in industrial applications. However, little research has been conducted on cyanobacteria This study was conducted to investigate the toxicity of ionic liquids ([Hmim]Cl) on Microcystis aeruginosa PCC7806. The EC50 (72 h) of [Hmim]Cl on the growth of Microcystis aeruginosa PCC 7806 was 10.624 ± 0.221 mg L−1. The possible mechanism of toxicity of [Hmim]Cl against M. aeruginosa PCC 7806 was evaluated by measuring cell growth, photosynthetic pigment contents, chlorophyll fluorescence transients, cell ultrastructure, and transcription of the microcystin-producing gene (mcyB). The concentrations of chlorophyll a and carotenoids were significantly reduced in treated M. aeruginosa cultures. The results of chlorophyll fluorescence transients showed that [Hmim]Cl could destruct the electron-accepting side of the photosystem II of M. aeruginosa PCC 7806. Transmission electron microscopy demonstrated cell damage including changes in the structure of the cell wall and cell membrane, thylakoid destruction, and nucleoid disassembly. The transcription of the mcyB gene was also inhibited under [Hmim]Cl stress. In summary, this study provides new insights into the toxicity of [Hmim]Cl on cyanobactreia.

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Growth inhibition and possible mechanism of oleamide against the toxin-producing cyanobacterium Microcystis aeruginosa NIES-843

Cited by 18 publications

References 32 publications

Physiological Responses of a Diazotrophic Cyanobacterium to Acidification of Paddy Floodwater: N2 Fixation, Photosynthesis, and Oxidative–Antioxidative Characteristics

Physiological Responses of a Diazotrophic Cyanobacterium to Acidification of Paddy Floodwater: N2 Fixation, Photosynthesis, and Oxidative–Antioxidative Characteristics

Combined allelopathic effects of Spirogyra (Zygnematales: Zygnemataceae) and Ceratophyllum demersum (Ceratophyllales: Ceratophyllaceae) on the growth of Microcystis aeruginosa (Chroocolales: Microcystaceae)

Inhibition Effect of Ionic Liquid [Hmim]Cl on Microcystis Growth and Toxin Production

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