Growth inhibition of Escherichia coli by dichloromethane in cells expressing dichloromethane dehalogenase/glutathione S-transferase

Evans, Gareth J. S.; Ferguson, Gail P.; Booth, Ian R.; Vuilleumier, Stéphane

doi:10.1099/00221287-146-11-2967

Cited by 20 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1). First, the strain must accommodate the protons and chloride produced intracellularly as a by-product of DCM dehalogenation (22)(23)(24). Additionally, the S-chloromethylglutathione intermediate formed during the dehalogenation reaction is highly reactive and mutagenic (25)(26)(27).…”

mentioning

confidence: 99%

Phylogeny Poorly Predicts the Utility of a Challenging Horizontally Transferred Gene in Methylobacterium Strains

Vuilleumier¹,

Bringel²,

Marx³

2014

Journal of Bacteriology

View full text Add to dashboard Cite

cHorizontal gene transfer plays a crucial role in microbial evolution. While much is known about the mechanisms that determine whether physical DNA can be transferred into a new host, the factors determining the utility of the transferred genes are less clear. We have explored this issue using dichloromethane consumption in Methylobacterium strains. Methylobacterium extorquens DM4 expresses a dichloromethane dehalogenase (DcmA) that has been acquired through horizontal gene transfer and allows the strain to grow on dichloromethane as the sole carbon and energy source. We transferred the dcmA gene into six Methylobacterium strains that include both close and distant evolutionary relatives. The transconjugants varied in their ability to grow on dichloromethane, but their fitness on dichloromethane did not correlate with the phylogeny of the parental strains or with any single tested physiological factor. This work highlights an important limiting factor in horizontal gene transfer, namely, the capacity of the recipient strain to accommodate the stress and metabolic disruption resulting from the acquisition of a new enzyme or pathway. Understanding these limitations may help to rationalize historical examples of horizontal transfer and aid deliberate genetic transfers in biotechnology for metabolic engineering.

show abstract

mentioning

confidence: 99%

Phylogeny Poorly Predicts the Utility of a Challenging Horizontally Transferred Gene in Methylobacterium Strains

Vuilleumier¹,

Bringel²,

Marx³

2014

Journal of Bacteriology

View full text Add to dashboard Cite

show abstract

“…Production of chloride ions likely requires efficient chloride efflux against a growing concentration gradient [5], but it is not yet known whether the well-characterised bacterial chloride channel [18] participates in chloride excretion in aerobic methylotrophic bacteria. Here, the energy dependence of chloride excretion into the medium was explored as a function of the addition of different uncoupling agents and electron transport chain inhibitors in cell suspensions of Mb.…”

Section: Resultsmentioning

confidence: 99%

“…extorquens AM1 to grow with DCM, when provided with dcmA functionally expressed from a plasmid [12], suggest that other proteins and genes are likely to be involved in growth with DCM. To date, microbial adaptive mechanisms to DCM remain to be elucidated, although it is clear that DCM-consuming bacteria excrete the protons and chloride anions produced by dehalogenation into the extracellular medium [5,7].…”

Section: Introduction *mentioning

confidence: 99%

Chloride‐associated adaptive response in aerobic methylotrophic dichloromethane‐utilising bacteria

Torgonskaya

Доронина

Hourcade

et al. 2011

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

Aerobic methylotrophic bacteria able to grow with dichloromethane (DCM) as the sole carbon and energy source possess a specific glutathione S-transferase, DCM dehalogenase, which transforms DCM to formaldehyde, used for biomass and energy production, and hydrochloric acid, which is excreted. Evidence is presented for chloride-specific responses for three DCM-degrading bacteria, Methylobacterium extorquens DM4, Methylopila helvetica DM6 and Albibacter methylovorans DM10. Chloride release into the medium was inhibited by sodium azide and m -chlorophenylhydrazone, suggesting an energy-dependent process. In contrast, only nigericin affected chloride excretion in Mb. extorquens DM4 and Mp. helvetica DM6, while valinomycin had the same effect in A. methylovorans DM10 only. Chloride ions stimulated DCM-dependent induction of DCM dehalogenase expression for Mp. helvetica DM6 and A. methylovorans DM10, and shortened the time for onset of chloride release into the medium. Striking chloride-containing structures were observed by electron microscopy and X-ray microanalysis on the cell surface of Mp. helvetica DM6 and A. methylovorans DM10 during growth with DCM, and with methanol in medium supplemented with sodium chloride. Taken together, these data suggest the existence of both general and specific chloride-associated adaptations in aerobic DCM-degrading bacteria.

show abstract

“…In this study pH values also decrease along with degradation of DCM. Methylotrophic bacteria, which are typical DCM degrading bacteria, metabolize DCM to formaldehyde, formic acid and then carbon dioxide under aerobic conditions 10 . Formaldehyde decomposes chemically or biologically to give formic acid.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Dichloromethane in the Groundwater at Illegal Waste Dump Site

Yimiti

Nakazawa

Hareyama

et al. 2011

Resources Processing

View full text Add to dashboard Cite

About 800,000 tones of wastes have been piled up or buried illegally at prefectural boundary area between Aomori and Iwate prefectures. Over 200 drums that contained organic solvent were buried and the solvent leaked from the drums resulting into the pollution of soils and groundwater. Dichloromethane (DCM) is one of pollutants and its concentration in groundwater was over 100 ppm. We have studied whether indigenous bacteria in the groundwater in the polluted site degraded DCM under aerobic conditions. Gas-tight vials with 10 ml of groundwater samples and 5 ml of headspace were shaken at 25°C. After 15 days of shaking, DCM concentration in a sample decreased from 108 ppm to 25 ppm. The bacteria population was subcultured using DCM as substrate without other organic matters. The bacteria population degraded DCM, and chloride concentration increased, indicating that DCM was degraded biologically. The effects of pH and TCE were investigated on the biodegradation of DCM. The experimental results indicated that bioremediation by indigenous bacteria is promising way to remedy DCM contaminated soil and groundwater at the illegal waste dump site.

show abstract

Growth inhibition of Escherichia coli by dichloromethane in cells expressing dichloromethane dehalogenase/glutathione S-transferase

Cited by 20 publications

References 38 publications

Phylogeny Poorly Predicts the Utility of a Challenging Horizontally Transferred Gene in Methylobacterium Strains

Phylogeny Poorly Predicts the Utility of a Challenging Horizontally Transferred Gene in Methylobacterium Strains

Chloride‐associated adaptive response in aerobic methylotrophic dichloromethane‐utilising bacteria

Biodegradation of Dichloromethane in the Groundwater at Illegal Waste Dump Site

Contact Info

Product

Resources

About